Impact of Time Interval between PGF2α Administration and Semen or Blood Plasma Collection on Spermatozoa Quality, Testosterone Level, cAMP, and Plasma ATP Level in Rats
Impact of Time Interval between PGF2α Administration and Semen or Blood Plasma Collection on Spermatozoa Quality, Testosterone Level, cAMP, and Plasma ATP Level in Rats
Husnurrizal Husnurrizal1,2, Hafizuddin Hafizuddin2*, Sri Wahyuni3, Cut Nila Thasmi2, Tongku Nizwan Siregar2,4
ABSTRACT
The effect of prostaglandin F2α (PGF2α) on spermatozoa quality improvement still shows inconsistent results. In addition, the mechanism leading to increased spermatozoa concentration after administration of PGF2 has not been clearly explained. Therefore, this research aimed to assess the impact of the time interval between PGF2α administration and semen collection on the enhancement of spermatozoa quality, testosterone, cyclic adenosine monophosphate (cAMP), and adenosine triphosphate (ATP) levels. A total of 15 rats (Rattus norvegicus) categorized into five treatment groups (n=3) were used in this study. In the control group (P0), the semen and blood samples were collected 30 minutes after a 0.5 ml NaCL injection. In groups P1, P2, P3, and P4, semen and blood samples were collected 30, 60, 90, and 120 minutes, respectively, after intraperitoneal injection of 2.5 mg PGF2α/kg BW. Upon treatment completion, all the rats were euthanized with Zoletil at 40 mg/kg BW. Microscopic examination of spermatozoa quality, including motility, concentration, viability (survival), and abnormalities, was conducted using spermatozoa from cauda epididymis. Simultaneously, testosterone, cAMP, and ATP were also assessed using blood plasma from blood samples. Administration of PGF2α significantly affected spermatozoa concentration (p<0.05) at the 90th minute (P3), increased motility (P<0.05) overall treatment times, enhanced viability (P<0.05) at the 30th and 60th minutes (P1 and P2), and reduced abnormalities (P<0.05) in all treatment groups, but it did not affect testosterone levels (P>0.05). The peak of cAMP concentration occurred at P1, while that of ATP occurred at P4 (P<0.05). The time interval between PGF2α administration and sample collection affected spermatozoa concentration, viability, abnormalities, ATP, and cAMP, whereas spermatozoa motility and testosterone concentration remained unaffected.
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