Research Article

 

Mechanisms of Hepato-Renal Protective Activity of Ocimum basilicum Leaf Extract against Paracetamol Toxicity in Rat Model

 

Ahmed M. Soliman1*, Hanan A. Rizk3, Mostafa A. Shalaby1, Ashraf A. Elkomy2

1Department of pharmacology, Faculty of Veterinary Medicine, Cairo University, Egypt; 2Department of pharmacology, Faculty of Veterinary Medicine, Benha University, Egypt; 3Department of pharmacology and toxicology, National Organization for Drug Control and Research (NODCAR), Egypt.

 

Received | January 03, 2020; Accepted | March 12, 2020; Published | March 20, 2020

*Correspondence | Ahmed M. Soliman, Department of Pharmacology, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt; Email: galalpharma@hotmail.com; galalpharma@cu.edu.eg

Citation | Soliman AM, Rizk MA, Shalaby MA, Elkomy AA (2020). Mechanisms of hepato-renal protective activity of Ocimum basilicum leaf extract against paracetamol toxicity in rat model. Adv. Anim. Vet. Sci. 8(4): 385-391.

DOI | http://dx.doi.org/10.17582/journal.aavs/2020/8.4.385.391

ISSN (Online) | 2307-8316; ISSN (Print) | 2309-3331

Copyright © 2020 Soliman et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

 

INTRODUCTION

 

The use of medicinal plants in folk medicine is an age-long practice in various parts of the globe for both preventive and curative purposes. Todays, it is estimated that about 80% of the world population relies on botanical preparations as medicine to meet their healthcare needs (Ogbera et al., 2010).

 

Liver is the largest organ in the body and plays a significant role in protecting various biological function and help in detoxification and excretion of various endogenous and exogenous compounds (Mohamed et al., 2010). The liver is often the target organ for many chemically induced injuries. Many oxidative reactions produce reactive metabolites that can induce oxidative stress and consequently liver damage or injury. The types of injury to the liver depend on the type of toxic agent, the severity of intoxication, and the type of exposure, whether acute or chronic toxicity (Comfort et al., 2013).

 

Kidneys has an important role in removing wastes like creatinine and urea, regulating the balance of electrolytes and controlling the body’s fluid balance (Walter, 2004). For the kidneys to carry out their normal functions they have to be in good condition both functionally and structurally (Thomas, 2005).

 

Paracetamol (PCM); is a widely used analgesic medication in many countries. An overdose of paracetamol is a frequent reason for liver and renal toxicity and possible death (Zhao et al., 1998). PRM is metabolized in the liver by cytochrome P450 to N-acetyl-p-benzoquinone imine (NAPQI). NAPQI reacts with glutathione (GSH), therefore overdoses of paracetamol may result in a depletion of hepatocellular GSH (Hinson et al., 2004). GSH exhaustion caused NAPQI to binds with cellular proteins leading to mitochondrial dysfunction, oxidative stress, lipid peroxidation, DNA fragmentation, massive hepatocytes necrosis, liver damage and death (Larson, 2007).

 

Ocimum basilicum (Basil) is an annual herb of the Lamiaceae family, which is widely cultivated in different regions of the world. O. basilicum was found to have numerous pharmacological activities. Basil leaves extracts have potent antioxidant, anti-aging, anticancer, antiviral, and antimicrobial properties (Akujobi et al., 2004; Manosroi et al., 2006; Almeida et al., 2007). It also possesses good antioxidant as well as potential anti-stress in experimental animals (Sethi et al., 2004). Leaves and flowering parts of O. basilicum are traditionally used as antispasmodic, aromatic, carminative, digestive, galactogogue, stomachic and tonic agents (Adiguzell et al., 2005). O. basilicum is known to have numerous pharmacological activities. Basil leave extracts have potent antioxidant, anti-aging, anticancer, antiviral, and antimicrobial properties (Dasguptazz et al., 2004; Bozin et al., 2006; Noor et al., 2019; Eftekhar et al., 2019). The volatile oils of O. basilicum are estragol, linalool, eugenol, methyl chavicol and small quantities of methyl cinnamate, cineole, apigenin, luteolin, orientin, vicenin, and other terpenes (Samudralwar and Garg, 1996). Consumption of O. basilicum or basil oil has been associated with a reduction of total cholesterol, low-density lipoprotein and triglycerides (Hicham et al., 2009)

 

Hence, the goal of the present research work was to examine if the hepato-renal toxicity induced by paracetamol can be ameliorated by the use of O. basilicum extract or otherwise.

 

MATERIALS AND METHODS

 

Medicinal plant

The fresh leaves of O. basilicum, were were collected from a garden of Faculty of Agriculture, Cairo University, Egypt. Leaves were rinsed with clean water to remove any foreign matter, air dried and grinded into fine powder.

 

Preparation of ethanolic extract

Organic solvent extraction of the O. basilicum leaves was carried out using 95% ethanol which is considered as very effective solvent in extracting the active ingredients of the plant according to method described by Effraim et al. (2000). This was done using Soxhlet apparatus, fifty g of plant leaves powder were soaked in 500 ml of 95% ethanol inside the flask. The extraction was carried out for 24 hours by heating temperature that kept the solvent at 50-60 °C until a clear and colorless solvent appeared in the extracting unit. After that, the extract was dried by using an electric oven at temperature 40-45 °C until semi-solid extract was obtained. The dried extract was placed in an incubator at 38-40 °C for complete dryness of the sample. The final extract was kept frozen at –20 °C until use.

 

Experimental rats

Male Wistar rats (185-210 g) were obtained from the Animal House, Faculty of Veterinary Medicine, Cairo University, Egypt. They were fed on a standard rat pellet and tap water was provided ad libitum. Rats were kept in plastic cages at room temperature 22-25°C. Experimental rats were acclimatized to the environment for two week prior to experimental use. This experiment was carried out according to the guidelines of the Institutional Animal Care and Use Committee, Cairo University, Approval Protocol No.: Cairo University-II-F-59-15 dated December/2018. (Protocol number 2211201809 / 2018).

 

Experimental protocol

Twenty four male Wistar rats were distributed into four groups of six rats each. Group I rats received 2 ml of distilled water for 30 days and served as a vehicle control. Rats in the group II served as paracetamol intoxicated control and were administered a single oral dose of paracetamol (500 mg/kg) (Zhang et al., 2013), 1 h after last dose of distilled water administration. Groups III and IV received ethanolic extract of O. basilicum in dose of 200 and 400 mg/kg, respectively, once daily for 30 consecutive days followed by a single oral administration of paracetamol (500 mg/kg), 1 h after the last O. basilicum extract administration.

 

Blood sampling

After 24 h of paracetamol administration, rats were anesthetized by intraperioneal dose (50mg/kg) of pentobarbital sodium (Nesdonal®). Blood samples (2 ml) from each rat was collected by puncturing tail vein with fine needle in sterilized dry centrifuge tube and left to clot for 30 min at room temperature. The clear sera obtained after centrifugation (3000 rpm for 15 min) were stored at 4 ºC till used and used for further biochemical estimation which was performed by using ready-made kits from Diamond Diagnostics Company (Egypt). Rats were then euthanized by large dose (60 mg/kg) of pentobarbital sodium and livers and kidneys were dissected out for histopathological examination.

 

Statistical analysis

Data were expressed as (mean ± SE) and analyzed using SPSS (Statistical Package for the Social Sciences, version 16.0, Illinois, Chicago, USA) and differences between the means were examined by Duncan’s multiple range test.

 

 

RESULTS

 

Oral administration of paracetamol at a dose of 500 mg/kg caused a significant increase in parameters of liver function (ALT, AST, bilirubin, total cholesterol, triglycerides, LDL-c) and a significant decrease in albumin and HDL-c in comparison with control values. These biochemical parameters are recorded in Table 1. Oral administration of paracetamol at a dose of 500 mg/kg caused a significant increase in kidney function parameters (creatinine, urea, Glucose, sodium and potassium) and significant decrease in total protein in comparison with control values, these biochemical parameters are recorded in Table 2. In O. basilicum extract treated groups these biochemical parameters were returned towards the normal values. Liver and renal oxidative stress marker and antioxidant parameters in control and different treated groups are mentioned in Tables 3 and 4, respectively.

 

 

DISCUSSION

 

Paracetamol induced hepatic injury is considered as one of the most commonly used model for hepatoprotectivity drug screening through estimation of serum cytoplasmic enzymes; aspartate aminotransferase (AST), alanine aminotransferase (ALT), serum albumin (SA), total bilirubin (TB), blood urea (BU) and serum creatinine (SC) activities is a useful as quantitative markers of the extent, type of hepatocellular and renal affection by paracetamol (Dash Deepak et al., 2007). In the current study, the significant elevation of these biochemical parameters in the rats treated with paracetamol, indicate the deterioration of the hepatic functions due to the toxic effects of the drug and consequently, they have been attributed to the damage of structural integrity of the liver, because these enzymes released into the circulation after autolytic breakdown or cellular necrosis (Zhang et al., 2009).

 

Paracetamol treated group of rats showed marked increase in serum creatinine and urea values as compared to the values of serum creatinine and urea in rats of healthy control group. Increased levels of serum creatinine and urea have been considered as index of assessing nephrotoxicity (Anwar et al., 1999; Ali et al., 2001). The elevated values in

 

Table 1: Effect of oral administration of ethanolic extract of Ocimum basilicum (200 and 400 mg/kg b.wt. on liver parameters in paracetamol induced hepato-renal toxicity in rats (n=6).

 

Parameters	Control	Paracetamol	Ocimum basilicum extract (200 mg/kg bwt) + paracetamol	Ocimum basilicum extract (400 mg/kg bwt) + paracetamol
AST (U/L)	113.6±3.56b	151.33±4.06a	133.3±10.69ab	128.63±1.55b
ALT (U/L)	25.60±0.58b	54.12±4.61a	38.99±2.43ab	34.34±2.71b
T. bilirubin (mg/dl)	0.47±0.03c	0.87±0.03a	0.67±0.07b	0.60±0.06bc
Albumin (g/dl)	5.15±0.28a	3.55±0.14b	4.15±0.27b	4.31±0.18b
Cholesterol (mg/dl)	66.33±5.04b	94.33±6.33a	82.67±3.17ab	71.33±4.33b
Triglycerides (mg/dl)	63.33±3.52b	103±2.88a	76.01±6.50b	75.33±5.84b
HDL-Chol (mg/dl)	46.67±0.67a	29.67±2.84b	39.33±0.67a	40.33±4.18a
LDL-Chol (mg/dl)	11±0.27b	44.07±2.769a	28.13±2.37ab	15.93±1.23b

 

a, b, c Mean values having different letters in the same row differ significantly P<0.05).

 

Table 2: Effect of oral administration of ethanolic extract of Ocimum basilicum (200 and 400 mg/kg b.wt. on kidney parameters in paracetamol induced hepato-renal toxicity in rats (n=6).

 

Parameters	Control	Paracetamol	Ocimum basilicum extract (200 mg/kg bwt) + paracetamol	Ocimum basilicum extract (400 mg/kg bwt)+ paracetamol
Creatinine (mg/dl)	1.13±0.09c	2.89±0.11a	1.85±0.04b	1.93±0.07b
Urea (mg/dl)	57.24±4.14b	70.81±5.41a	40.57±2.01c	26.41±0.55d
T. protein (g/dl)	20.25±1.77a	15.69±1.62b	12.81±0.92b	7.78±0.28c
Glucose (mg/dl)	49.75±2.76b	108.28±9.32a	65.93±2.76b	63.72±3.24b

 

a, b, c, d Mean values having different letters in the same row differ significantly (P<0.05).

 

Table 3: Antioxidant activity of Ocimum basilicum (200 and 400 mg/kg b.wt) in liver tissues (n=6).

 

Parameters	Control	Paracetamol	Ocimum basilicum extract (200 mg/kg bwt) + paracetamol	Ocimum basilicum extract (400 mg/kg bwt) + paracetamol
CAT (U/g.tissue)	54.76±4.58a	12.60±0.66c	26.89±1.45b	22.13±0.71b
SOD (U/g.tissue)	47.24±2.17a	19.46±1.72c	27.88±2.54bc	33.40±2.54b
MDA (U/g.tissue)	35.27±2.73c	94.24±4.81a	67.88±3.36b	42.58±3.25c

 

a, b, c Mean values having different letters in the same row differ significantly (P<0.05).

 

Table 4: Antioxidant activity of Ocimum basilicum (200 and 400 mg/kg b.wt) in kidney tissues (n=6).

 

Parameters	Control	Paracetamol	Ocimum basilicum extract (200 mg/kg bwt)+ paracetamol	Ocimum basilicum extract (400 mg/kg bwt) + paracetamol
CAT (U/g.tissue)	73.80±6.29a	23.80±1.30d	54.76±4.75b	40.47±2.38c
SOD (U/g.tissue)	122.15±8.38a	73.62±2.89c	100.95±2.88ab	99.61±4.35ab
MDA (U/g.tissue)	18.22±1.37c	76.20±6.98a	66.25±6.32a	29.90±1.80b

 

a, b, c Mean values having different letters in the same row differ significantly (P<0.05).

 

experimental rats indicate the severity of kidney damage by the Paracetamol. Necrosis of liver and kidney cells observed in the present study might be responsible for elevation of these biomarker enzymes. Values of serum creatinine and urea in groups of rats treated with O. basilicum extract showed significant improvement and serum creatinine and urea values. Previous studies reported the significant decrease in serum creatinine level by treatment with various herbal preparations and extracts which was increased in Paracetamol induced nephrotoxic rats support the findings of present study (Gulnaz et al., 2010; Palani et al., 2010).

 

This study demonstrated that, Alterations in serum levels of hepatic transaminases (AST and ALT) were used as markers for liver damage and injury. In our study, there was a significant increase in AST and ALT levels in paracetamol administered rats. O. basilicum administration ameliorated both liver and kidney changes confirming the protective role of O. basilicum against hepatic toxicity induced by paracetamol overdose and that is coincided with results of Li et al. (2013). Moreover, it was reported that O. basilicum supplementation improved liver histopathology and showed an improvement in hepatic toxicity.

 

Lipid peroxidation and antioxidant potency (SOD and catalase) of cells were used to assess the degree of hepatic cell stability and integrity (Evans et al., 2002). Oxidative damage caused by paracetamol overdose was significantly attenuated by O. basilicum administration.

 

Ocimum basilicum extract produced significant decrease in triglycerides, total cholesterol, LDL-cholesterol and significant increase in HDL-cholesterol concentrations in serum of O. basilicum extract treated groups than recorded in control group. Also, improve the lipid profile after paracetamol toxicity. O. basilicum treatment attenuated serum lipid profile. This may be due to the antihyperlipidemic action of components of O. basilicum leaves. Suanarunsawat et al. (2009) mentioned that the anti-hyperlipidemic activity of O.basilicum may be due to the suppression of liver lipid synthesis. These results were consistent with those obtained by Elkomy et al. (2016) who recorded that, paracetamol overdose caused significant increase in serum total cholesterol, serum triglycerides, low density lipoprotein, total bilirubin with a reduction of albumin and high density lipoprotein cholesterol.

 

Previous phytochemical screening revealed that O. basilicum is a rich source of polyphenols which have potential therapeutic and health promoting effects (Garcia-Lafuente et al., 2009). Polyphenolic compounds are most abundant natural antioxidants and their radical scavenging capabilities play an important role in preventing many chronic diseases (Garcia-Lafuente et al., 2009; Rathee et al., 2009). The main groups of polyphenols are: flavonoids, phenolic acids, phenolic alcohols, stilbenes, and lignans (D’Archivio et al., 2007). The antioxidant activity of the plant may be due to flavonoids which have shown to possess various biological properties related to antioxidant mechanism (Marzouk, 2009), or the effect may be due to Caffeic acid (CA): which is another content in the leaf of the O. Basilicum which has antioxidant, anti-inflammatory, and cancer chemopreventive activities (Neradil et al., 2003). Other constituent of O. Basilicum is a p-coumaric acid (pCA) which found in high concentration which possessed radical scavenging and antioxidant (Yeh and Yen, 2003). Recent studies reported that the antioxidant activity of Basil (Ocimum basilicum leaves) could be attributed to its bioactive phenolic compounds in leaves and flowers (Prinsi et al., 2020). In addition, other researchers (Noor et al., 2019; Eftekha et al., 2019) reported that ocimum basilicum has high antioxidant activity against oxidative stress. It also has immunomodulatory, antinflammatory, antiapoptotic and cell regeneration effects (Ibrahim et al., 2020).

 

All above mechanisms may acceleration of liver cells regeneration and reducing the leakage of the above enzymes in to blood. On the other hand ethanolic leaves extract of O. Basilicum caused decreased in the level of cholesterol and this result in agreement with previous studies (Amrani et al., 2006).

 

Histopathological findings of tissue damage induced by paracetamol in the liver showed sever congestion and dilatation in the portal vein associated with inflammatory cells infiltration and edema in the periductal tissue surrounding the cystic dilated bile ducts and this coincides with Roomi et al. (2008) who found mild and significant lobular focal hepatitis in paracetamol studied group. Regarding renal histological finding, massive inflammatory cells infiltration was detected in between the degenerated renal tubules at the cortex. These results are in agreement with those of Pathan et al. (2013) who reported that kidneys of paracetamol-treated rats, showed loss of renal tubular architecture with rearrangement of renal tubules and glomeruli. Kidney sections showed diffuse degenerative changes in the sections and the renal tubules showed cellular swelling invariably with narrowing of lumen to great extent. Histopathological findings of paracetamol on liver and kidney were also markedly decreased by co-administration of O. basilicum extract.

 

CONCLUSIONS

 

Ocimum basilicum leaf extract normalized all the treated biochemical parameters of liver and kidney function induced by paracetamol to nearly to normal levels. It also ameliorated the histopatholocal lesions seen in livers and kidneys. Thus the ethanol extract of Ocimum basilicum minimizes the hepato-renal toxicity induced by paracetamol, thereby suggesting its use as a potent hepatoprotective nephroprotective agent.

 

ACKNOWLEDGMENT

 

The authors wish to thank Prof. Dr: Adel Bakeer (Department of Pathology, Faculty of Veterinary Medicine, Cairo University, Egypt) for his comments on histopathological study.

 

AUTHORS CONTRIBUTION

 

Mostafa A. Shalaby and Ashraf El-Komy designed and planned this study. Ahmed M. Soliman and Hanan A. Rizk prepared the plant extract and performed the experimental work, samples collection and all laboratory tests. All authors shared manuscript writing, drafted and approved the final manuscript.

 

Conflict of interest

 

The authors declare that there is no conflict of interests regarding the publication of this article.

 

REFERENCES

 

• Adiguzell A, Glluce M, Sengul M, Ogutcu H, Sahin F, Karaman I (2005). Antimicrobial effects of Ocimum basilicum (Labiatae) extract. Turk. J. Biol. 29: 155-160.

• Ahmed HA, Sadek KM, Taha AE (2015). Impact of two herbal seeds supplementation on growth performance and some biochemical blood and tissue parameters of broiler chickens. Int. Sch. Sci. Res. Innov., 9(3): 279- 284.

• Akujobi C, Anyanwu BN, Onyeze C, Ibekwe VI (2004). Antibacterial activities and preliminary phytochemical screening of four medical plants. J. Appl. Sci. 7(3): 4328-4338.

• Ali B, Ben H, Ismail TH, Basheer AA (2001). Sex related differences in the susceptibility of rat to gentamicin nephrotoxicity: influence of gonadectomy and hormonal replacement therapy. Ind. J. Pharmacol. 33: 369-373.

• Almeida I, Alviano DS, Vieira DP, Alves PB, Blank AF, Lopes AH, Alviano CS, Rosa MS (2007). Antigiardial activity of Ocimum basilicum essential oil. Parasitol. Res. 101(2): 443-452. https://doi.org/10.1007/s00436-007-0502-2

• Amrani S, Harnafi H, Bouanani NE, Aziz M, Caid HS, Manfredini S, Besco E, Napolitano M, Bravo E (2006). Hypolipidaemic activity of aqueous Ocimum Basilicum extract in acute hyperlipidaemia induced by triton WR-1339 in rats and its antioxidant property. J. Pharm. Med. 20(12): 1040–1045. https://doi.org/10.1002/ptr.1961

• Anwar S, Khan NA, Amin KMY, Ahmad G (1999). Effects of banadiq-al buzoor in some renal disorders. Hamdard Medicus, Vol. XLII. Hamdard Foundation, Karachi, Pakistan. 4: 31-36.

• Bozin B, Mimica-Dukic N, Simin N, Anackov G (2006). Characterization of the volatile composition of essential oils of some lamiaceae spices and the antimicrobial and antioxidant activities of the entire oils. J. Agric. Food Chem. 54: 1822–1828. https://doi.org/10.1021/jf051922u

• Comfort JA, Augusta AE, Gbadebo EA, Margaret OO and Emmanuel NM (2013). N-Nitrosation of dimethylamine hydrochloride and its toxicology in the wistar rats fed different levels of dietary protein. Eur. J. Exp. Biol. 3(2): 94-103.

• D’Archivio M, Filesi C, Di Benedetto R, Gargiulo R, Giovannini C, Masella R (2007). Polyphenols, dietary sources and bioavailability. Ann. 1st. Super. Sanita, 43: 348-361.

• Dasguptazz T, Rao AR, Yadava PK (2004). Chemo-modulatory efficacy of basil leaf (Ocimum basilicum) on drug metabolizing and antioxidant enzymes, and on carcinogen-induced skin and forestomach papillomagenesis. Phytomedicine. 11(2-3): 139–151. https://doi.org/10.1078/0944-7113-00289

• Dash Deepak K, Yeligar Veerendra C, Nayak Siva S, Tirtha Ghosh, Rajalingam D (2007). Evaluation of hepatoprotective and antioxidant activity of Ichnocarpus frutescens (Linn.) R.Br. on paracetamol – induced hepatotoxicity in rats. Trop. J. Pharm. Res. 6: 755–765. https://doi.org/10.4314/tjpr.v6i3.14656

• Effraim ID, Salami HA, Osewa TS (2000). The effect of aqueous leaf extract of Ocimum gratissimum on haematological and biochemical parameters in rabbits. Afr. J. Biomed. Res. pp. 175-179.

• Eftekhar N, Moghimi A, Mohammadian Roshan N, Saadat S, Boskabady MH (2019). Immunomodulatory and anti-inflammatory effects of hydro-ethanolic extract of Ocimum basilicum leaves and its effect on lung pathological changes in an ovalbumin-induced rat model of asthma. BMC Complement. Altern. Med. 19(1): 349. https://doi.org/10.1186/s12906-019-2765-4

• Elkomy A, Aboubakr M, Soliman A, Abdeen A, Abdelkader A, Hekal H (2016). Paracetamol induced hepato-renal toxicity and amelioration by cinnamon in Rats. IJPT. 4 (2): 187-190. https://doi.org/10.14419/ijpt.v4i2.6529

• Evans JL, Goldfine ID, Maddux BA, Grodsky GM (2002). Oxidative stress and stress activated signaling pathways: a unifying hypothesis of type 2 diabetes. Endo Rev. 23: 599–622. https://doi.org/10.1210/er.2001-0039

• Garcia-Lafuente A, Guillamon E, Villares A, Rostagno MA, Martinez JA (2009). Flavonoids as anti-inflammatory agents: implications in cancer and cardiovascular disease. Inflamm. Res. 58: 537-552. https://doi.org/10.1007/s00011-009-0037-3

• Gulnaz H, Tahir M, Muni B, Sami W (2010). Protective effects of garlic oil on acetaminophen induced nephrotoxicity in male Wistar rats. Biomedica. 26: 9-15.

• Hicham H, Mohammed A, Souliman A (2009). Sweet basil (Ocimum basilicum L.) improves lipid metabolism in hypercholesterolemic rats. J. Clin. Nutr. Metab. 4: 181-186. https://doi.org/10.1016/j.eclnm.2009.05.011

• Hinson JA, Reid AB, McCullough SS, James LP (2004). Acetaminophen-induced hepatotoxicity: role of metabolic activation, reactive oxygen/nitrogen species, and mitochondrial permeability transition. Drug. Metab. Rev. 36: 805–822. https://doi.org/10.1081/DMR-200033494

• Ibrahim RYM, Mansour SM, Elkady WM (2020). Phytochemical profile and protective effect of Ocimum basilicum aqueous extract in doxorubicin/irradiation‐induced testicular injury. J. Pharm. Pharmacol. 72(1): 101-110. https://doi.org/10.1111/jphp.13175

• Larson AM (2007). Acetaminophen hepatotoxicity. Clin. Liver Dis. 11: 525–548. https://doi.org/10.1016/j.cld.2007.06.006

• Li G, Chen JB, Wang C, Xu Z, Nie H, Qin XY, Chen XM, Gong Q (2013). Curcumin protects against acetaminophen-induced apoptosis in hepatic injury. World J. Gastroenterol. 19: 7440–7446. https://doi.org/10.3748/wjg.v19.i42.7440

• Manosroi J, Dhumtanom P, Manosroi A (2006). Anti-proliferative activity of essential oil extracted from Thai medicinal plants on KB and P388 cell lines. Cancer Lett. 235 (1): 114-120. https://doi.org/10.1016/j.canlet.2005.04.021

• Marzouk AM (2009). Hepatoprotective Triterpenes from Hairy Root Cultures of Ocimum basilicum L. Z. Naturforsch. 64 c: 201 – 209. https://doi.org/10.1515/znc-2009-3-409

• Mohamed STS, Madhusudhana C, Chetty S, Ramkanth VST, Rajan KM (2010). Hepatoprotective herbs. Int. J. Res. Pharm. Sci. 1: 1-5.

• Neradil J, Veselska R, Slanina J (2003). UVC-protective effect of caffeic acid on normal and transformed human skin cells in vitro. Folia Biol. (Praha). 49: 197-202.

• Noor ZI, Ahmed D, Rehman HM, Qamar MT, Froeyen M, Ahmad S, Mirza MU (2019). In Vitro Antidiabetic, Anti-Obesity and Antioxidant Analysis of Ocimum basilicum Aerial Biomass and in Silico Molecular Docking Simulations with Alpha-Amylase and Lipase Enzymes. Biology, 8(4): 92. https://doi.org/10.3390/biology8040092

• Ogbera AO, Dada O, Adeyeye F, Jewo PI (2010). Complementary and alternative medicine use in diabetes mellitus. West Afr. J. Med. 29(3): 158-162. https://doi.org/10.4314/wajm.v29i3.68213

• Palani S, Raja S, Kumar RP, Parameswaran P, Kumar BS (2010). Therapeutic efficacy of Acorus calamus on acetaminophen induced nephrotoxicity and oxidative stress in male albino rats. Acta Pharm. Sci. 52: 89-100.

• Pathan MM, Khan MA, Moregaonkar SD, Somkuwar AP, Somkuw AR, Gaikwad NZ (2013). Amelioration of paracetamol induced nephrotoxixity by Maytenus emagrinata in male wistar male rats. Int. J. Pharm. Pharm. Sci. 5(4): 471-474.

• Prinsi B, Morgutti S, Negrini N, Faoro F, Espen L (2020). Insight into Composition of Bioactive Phenolic Compounds in Leaves and Flowers of Green and Purple Basil. Plants. 9(1): 22-37. https://doi.org/10.3390/plants9010022

• Rathee P, Chaudhary H, Rathee S, Rathee D, Kumar V, Kohli K (2009). Mechanism of action of flavonoids as anti-inflammatory agents: a review. Inflamm. Allergy Drug. Targets. 8: 229-235. https://doi.org/10.2174/187152809788681029

• Roomi MW, Kalinovsky T, Ivanov V, Rath M, Niedzwiecki A (2008). A nutrient mixture prevents acetaminophen hepatic and renal toxicity in ICR mice. Hum. Exp. Toxicol. 27: 223-230. https://doi.org/10.1177/0960327108090276

• Samudralwar DL, Garg AN (1996). Minor and trace elemental determination in the Indian herbal and other medicinal preparations. Biol. Trace Elem. Res. 54: 113-121. https://doi.org/10.1007/BF02786258

• Sethi, J, Sood S, Seth S, Talwar A (2004). Evaluation of hypoglycemic and antioxidant effect of Ocimum sanctum. Indian J. Clin. Biochem. 19: 152-155. https://doi.org/10.1007/BF02894276

• Suanarunsawat T, Wacharaporn DNA, Songsak T, Rattanamahaphoom J (2009). Anti-lipidemic actions of essential oil extracted from Ocimum sanctum L. leaves in rats fed with high cholesterol diet. J. Appl. Biomed. 7: 45–53.

• Thomas SR (2005). Modelling and simulation of the kidney. J. Biol. Phys. Chem. 5: 70-83.

• Walter FB (2004). Medical physiology: A cellular and molecular approach. Elsevier/Saunders. ISBN 1-4160-2328-3.

• Yeh CT, Yen GC (2003). Effects of phenolic acids on human phenol-sulfotransferases in relation to their antioxidant activity. J. Agric. Food Chem. 51: 1474-1479. https://doi.org/10.1021/jf0208132

• Zhang L, Gavin T, Geohagen BC, Liu Q, Downey KJ, LoPachin RM (2013). Protective properties of 2-acetylcyclopentanone in a mouse model of acetaminophen hepatotoxicity. J. Pharmacol. Exp. Ther. 346(2): 259–269. https://doi.org/10.1124/jpet.113.205435

• Zhang R, Hu Y, Yuan J, Wu D (2009). Effects of Puerariae radix extract on the increasing intestinal permeability in rat with alcohol-induced liver injury. J. Ethnopharmacol. 126: 207-214. https://doi.org/10.1016/j.jep.2009.08.044

• Zhao C, Sheryl D, Zhou YX (1998). Effects of combined use of diallyl disulfide and Nacetyl-cysteine on acetaminophen hepatotoxicity in betanaphthoflavone pretreated mice. World J. Gastroenterol. 4: 112–116. https://doi.org/10.3748/wjg.v4.i2.112