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Advances in Animal and Veterinary Sciences

AAVS_7_s2_51-56

 

 

Research Article

 

Molecular Characterization of Listeria Monocytogenes Isolated from Different Animal-Origin Food Items from Urban and Rural Areas

 

Azza S. El-Demerdash1* and Mona T. Raslan2

1Animal Health Research Institute, Zagazig, Egypt; 2Animal Health Research Institute, Dokki, Egypt

 

Abstract | Listeriosis is classified as the third main cause of food-borne diseases and it is one of the significant bacterial zoonotic infections causing high fatality rates worldwide. The aim of this research was to isolate and characterize Listeria monocytogenes (L. monocytogenes) from different food items in Sharkia Province, Egypt. A total of 200 food samples were collected aseptically from local markets and subjected to the phenotypic and genotypic characterization of L. monocytogenes. Listeria species were isolated from 28 (56%), 9 (18%), 3 (6%) and 5 (10%) of the minced meat, poultry meat, tilapia fish, and raw milk samples, respectively. Overall, L. monocytogenes were isolated from 12 and 8 samples from urban and rural areas with percentages of 6 and 4%, respectively. Of interest, inlA, actA, prfA, and hlyA virulence genes were detected in all 20 L. monocytogenes isolates by multiplex PCR. In conclusion, L. monocytogenes is an organism of public health implications, and its recovery from the food samples sold at retail outlets indicates a breach of quality assurance.

 

Keywords | L. monocytogenes, Food, Multiplex PCR, Virulence genes

 

Received | September 11, 2019; Accepted | October 06, 2019; Published | October 10, 2019

*Correspondence | Azza S. El-Demerdash, Animal Health Research Institute, Zagazig, Egypt; Email: Dr.azzasalah@yahoo.com

Citation | El-Demerdash AS and Raslan MT (2019). Molecular characterization of listeria monocytogenes isolated from different animal-origin food items from urban and rural areas. Adv. Anim. Vet. Sci. 7(s2): 51-56.

DOI | http://dx.doi.org/10.17582/journal.aavs/2019/7.s2.51.56

ISSN (Online) | 2307-8316; ISSN (Print) | 2309-3331

Copyright © 2019 El-Demerdash et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

 

INTRODUCTION

 

Listeria monocytogenes (L. monocytogenes) is an opportunistic microorganism which causes extreme food-borne infirmity, commonly known as Listeriosis (Gray et al., 2006). It is ranked as one of the major causative agents for high mortality rates among all foodborne bacterial infections. The instance of listeriosis has been sporadic in African countries. Recently, there has been an expansion in the prevalence of human listeriosis, comprising food contamination in Egypt which iteratively detected in several dairy products, ready-to-eat foods, fish and fish products (Ismaiel et al., 2014; Kamar et al., 2014; Mohamed et al., 2016).

 

L. monocytogenes is a rod-shaped Gram-positive bacterium that is motile and non-spore-forming. It belongs to the facultative intracellular bacteria that attack, replicate and multiply in an assortment of cells through specific mechanism (Vasconcelos et al., 2008). After ingestion of contaminated food with L. monocytogenes, it crosses the intestinal barrier to the mesenteric lymph nodes. The bacteria disseminate via the bloodstream to the liver and spleen where they multiply. The bacteria are now able to reach the brain and, in case of pregnant women, also the placenta and the fetus. Entry into host cells is mediated by the internalins (Inl). Inside the cell, the bacterium is surrounded by a vacuole from which it rapidly escapes via listeriolysin O (LLO) by the action of hlyA gene (Churchill et al., 2006). Then, L. monocytogenes replicates in the cytoplasm and assembles an actin tail at the posterior pole of the bacterium where the surface protein (actA) is located. The actin tail is used to move in the cytoplasm and to spread into the neighboring cells via membrane protrusions (Vázquez-Boland et al., 2001; Liu et al., 2007a; Aurora et al., 2008; Piana et al., 2012).

 

Expression of the virulence genes mentioned above requires a transcriptional activator called prfA which is encoded by a gene in the same genomic cluster (Kathariou, 2002).

 

Therefore, dependable recognition of pathogenic L. monocytogenes has been proposed to be perfectly established upon the disclosure of virulence indicators (InlA, hlyA, actA, and prfA), but the determination of single virulence gene by uniplex PCR is not constantly adequate to recognize pathogenic L. monocytogenes (Di Ciccio et al., 2012; Jadhav et al., 2012). Moreover, the presence of spontaneous mutations may lead to the absence of one or more specific virulence determinants for some L. monocytogenes strains (Soni et al., 2010; Clayton et al., 2011; Di Ciccio et al., 2012).

 

In this way, the simultaneous discovery of a virulent pattern in one step minimizes the working period and will be utilized in a huge scanning for distinguishing the pathogenic listeria isolates. Multiplex PCR encompassing determination of these virulence genes has advanced as a zone of extraordinary potency (Chen and Knabel, 2007; Kérouanton et al., 2010; Shen et al., 2013).

 

Taking into consideration all these aspects, the present study investigated the prevalence of L. monocytogenes in animal-origin food items from different sources and areas in addition to determining some virulence determinants of isolates.

 

MATERIALS AND METHODS

 

Sampling

A total of 200 samples from different sources (minced meat, poultry meat, tilapia fish and raw milk) - 50 each- were collected from different urban and rural areas -100 each- in Sharkia province, Egypt.

 

Isolation and identification of L. monocytogenes

The International Organization for Standardization methods were used for isolation and identification of L. monocytogenes from different food specimens ( ISO 11290-1: 1996/Amd 1 2004) by the aid of enrichment broths as half Fraser and full Fraser; Oxford and Agar Listeria Ottaviani and Agosti (ALOA) agar. The purification was achieved onto Tryptone Soya Yeast Extract agar (TSYEA). Pinpoint colonies presumptively suspected as listeria isolates on TSYEA plates were sequentially submitted to comprehensive analyses including beta hemolysis on sheep blood agar and some biochemical tests such as sugar fermentation on rhamnose and xylose broths, reactions on triple sugar iron agar and oxidase (Hitchins, 2003).

 

Molecular investigation and characterization

The methodology of QIAGEN DNeasy was utilized to extract the DNA of the L. monocytogenes isolates with a minor adjustment in centrifugation parameters that is multiplying the centrifugation time. Then, PCR amplification was achieved with a PTC-100 programmable thermal cycler (Peltier-Effect cycling, MJ, UK) with adjusting the final volume of the reaction mixture to 50 µL consisting of 25 µL of DreamTaq TM Green Master Mix (2X) (Fermentas, USA), 0.2 µL of 100 pmole of each primer (Sigma, USA), 5 µLof template DNA and water nuclease-free up to 50 µL.

 

The multiplex PCR protocols were used to screen the presence or absence of hlyA, actA, prfA, and inlA virulence-associated genes among obtained L. monocytogenes isolates. The primers used for amplifications were selected from a previous research (Di Ciccio et al., 2012) and their sequences are listed in Table 1.

 

The PCR assay was established utilizing standard strain as a positive control (ATCC 19155) and negative control (PCR mixture without DNA template). Initial denaturation was achieved at 94 °C for 3 min followed by 35 cycles of denaturation at 94°C for 1 min, annealing at 60°C for 2 min and extension at 72°C for 1 min 30 s. Then, the final extension was processed at 72 °C for 15 min (Di Ciccio et al., 2012).

 

The PCR products were separated by electrophoresis on 1.5% agarose gel (Applichem, Germany, GmbH). The gel was photographed by a gel documentation framework (Alpha Innotech, Biometra) and the data were analyzed by computer software.

 

Statistical data analysis

The obtained results were analyzed utilizing the descriptive analysis of the Pearson Correlation Test. Significance was recorded at P< 0.05 (Bryman and Cramer, 2002)

 

RESULTS

 

In total, 45 (22.5%) Listeria species were isolated from the 200 samples tested in our study when platted onto Oxford agar. Colonies are small (1.0 mm in diameter), grayish, encircled with a black zone.

 

On ALOA medium, the listeria isolates exhibited bluish-green colonies. Then, pathogenic Listeria spp can be differentiated from other Listeria spp through creating of phosphatidylinositol-specific phospholipase C (PI-PLC), that is demonstrated by the formation of opaque halos around the colonies.

 

In the present study, minced meat and tilapia fish showed the highest and lowest significant prevalence rates (P< 0.05) among the obtained samples with percentages 56 and 6%, respectively (Table 2).

 

 

Table 1: Primers used for PCR reactions

 

Target gene Primer name Sequence (5=،3)

Amplicon size (bp)
hlyA

HlyAF

HlyAR

CCT AAG ACG CCA ATC GAA

AAG CGC TTG CAA CTG CTC

702
actA

ActAF

ActAR

GAC GAA AAT CCC GAA GTG AA

CTA GCG AAG GTG CTG TTT CC

268
prfA

PrfAF

PrfAR

CTG TTG GAG CTC TTC TTG GTG AAGCAA TCG

AGC AAC CTC GGA ACC ATA TAC TAACTC

1060
inlA

InlAF

InlAR

CCT AGC AGG TCT AAC CGC AC

TCG CTA ATT TGG TTA TGC CC

255

 

Table 2: Detection of Listeria monocytogenes in different food samples

Food item Examined samples no. Listeria spp. positive (%) No. (%) of identified L. monocytogenes isolates
Rural Urban
Minced meat 50

28 (56%)a

2 (4%) b

7 (14%)a

Poultry meat 50

9 (18%) b

3 (6%) a

2 (4%) b

Tilapia fish 50

3 (6%) d

1 (2%) c

2 (4%) b

Raw milk 50

5 (10%)c

2 (4%)b

1 (2%)b

Total 200 45 (22.5%) 8 (4%) 12 (6%)

 

Groups within the same column carrying different superscript are significantly different at (P< 0.05).

 

The dissemination of L. monocytogenes isolates among different types of samples revealed that 9 (18%) of the recovered isolates were from minced meat, 5 (10%) from poultry meat and 3 (6%) from each tilapia fish and raw milk. Totally, the overall prevalence of L. monocytogenes among investigated samples was 10%. The correlation test verified that there was a significant association between the isolation rate for L. monocytogenes and the sample type (P<0.05). Moreover, most isolates were obtained from urban areas with a prevalence rate of 6%.

 

Regarding multiplex PCR results, All 20 isolates of L. monocytogenes tested for the presence/absence of examined virulence genes harbored all genes and gave their characteristic bands as shown in Figure 1.

 

DISCUSSION

 

Listeriosis has been remarked to be one of the emergent zoonotic diseases in recent years, being signed basically through the utilization of contaminated foods and food products. Expanding proofs propose that fundamental aspects of human listeriosis cases are owing to the foodborne transmission of L. monocytogenes (Molla et al., 2004). It remains to be of a great concern to the food manufacturing and regulating agencies worldwide, to the researchers in different authorities, and to the customers of ready to eat foods. Sporadic instances of listeriosis still to appear in different countries and there have been numerous outbreaks of the disease related to food in Egypt (Kamar et al., 2014).

 

In this assessment, 45 (22.5%) listeria isolates were recovered on ALOA medium from the 200 samples tested giving bluish-green colonies because of the prevalence of a chromogenic compound X-glucosidase, that recognizes b-glucosidase existing in all Listeria spp. This ratio was relatively higher than previous studies in Brazil (16.7%), Iran (20.3%), and Ghana (12.2%) (Monteiro et al., 2013; Haj Hosseini et al., 2014; Kwarteng and Wuni, 2018).

This difference in the obtained data might be because of the divergence in food type understudy, analyzed specimens number and geographical location (Nayakv et al., 2015).

 

Out of 50 minced meat specimens inspected, more than half (28/50) were contaminated with Listeria species. It may be attributed to the subjection of beef meat samples to insignificant hygienic conditions, throughout slaughtering, evisceration, processing, and vending. This data is in concurrence with the prior reports previously mentioned (Molla et al., 2004; Gebretsadik et al., 2011).

 

The second source for Listeria spp in our study was the poultry meat with a prevalence rate of 18%; five isolates (10%) were identified as L. monocytogenes. This is nearly by investigations directed in Greece (Sakaridis et al., 2011) and Iran (Zeinali et al., 2017) where L. monocytogenes were isolated from poultry meat with percentages of 38% and 18%, respectively representing contamination evidence linking live production and/or processing environments.

 

Regarding milk samples, the prevalence of Listeria species differs frequently among various survey reports and has been ascribed to factors such as geographic (ecological) conditions, farm size and management practices (Claeys et al., 2013). In our study, the frequency of Listeria spp. in raw milk was 10%, while in a previous report carried out in Ethiopia (Seyoum et al., 2015) a predominance of over 28% of Listeria spp was detailed. Prior studies mentioned that L. monocytogenes prevalence rates can vary from 0% in raw cow milk (Huehn et al., 2010) to more than 45% (De Reu et al., 2004; El Marnissi et al., 2013).

 

All three tilapia fish isolates obtained in this study were identified as L. monocytogenes. The previous literatures demonstrated relatively high incidence rates of L. monocytogenes in seafood sample; 7.7% in Iran (Momtaz and Yadollahi, 2013) and 7% in Egypt (Edris et al., 2014). There are two sources of fish contamination with Listeria species, that incorporates; the attack of listeria from intestinal contents to other fish tissues like muscles (Ertacs and cSeker, 2005), and cross-contamination (fish manipulation, using contaminated equipment and inappropriate transport) (Gudbjörnsdóttir et al., 2004).

 

Inclusively, the isolation rate of L. monocytogenes differed in various regions, giving an overall incidence of 10% (20/200). Moreover, the urban area showed the highest prevalence (6%), even though (Kersting et al., 2010; Amajoud et al., 2018) demonstrated that L. monocytogenes were isolated and overrepresented in high percentages from rural environments. The possible explanation for this discrepancy could be due to the difference in packing methods, handling and geographical difference (Seyoum et al., 2015; Wu et al., 2015).

 

Concerning PCR results, all genes playing a key role in the pathogenesis of L. monocytogenes (prfA, hlyA, actA, and inlA) were detected in all examined isolates as shown in Figure 1. These data are in concurrence with preceding data conducted in Italy, where prfA and actA genes were predominant (100%) among L. monocytogenes isolates, followed by hlyA (93%) and inlA (60%) genes (Coroneo et al., 2016) illustrating that the pathogenicity island genes LIPI-1, and the inl genes, are invariable parts of the genome of L. monocytogenes (Doumith et al., 2004; Gelbíčová et al., 2015). Moreover, the presence of the hlyA gene in all isolates encodes pore-forming listeriolysin O toxin which necessary for bacterial escape from the phagosomes of host cells into the cytosol (Camejo et al., 2011).

 

Henriques et al. (1998) detected hlyA genes and actA from L. monocytogenes isolates from all Portuguese ready-to-eat meat samples, which is similar to the findings of this study. Listeriolysin O and actA are associated with the bacterium’s capability of passing the intestinal barrier, cell to cell spread and motility, cell invasion, and intracellular parasitism (Liu et al., 2007b; Rawool et al., 2007).

 

The high frequencies of virulence genes in the bacterial strains of our study enable adhesion, invasion, and epithelial damage to the human digestive system (Momtaz and Yadollahi, 2013).

 

Moreover, these results indicate that these isolates possess the properties of virulent strain and their sequences may be further investigated to explore the differences between pathogenic and less pathogenic strains (Abdellrazeq et al., 2014). Thus, the presence of these genes proposes the incidence of a critical public health risk correlated with the consumption of examined food samples sold in Sharkia Governorate contaminated with L. monocytogenes.

 

On another hand, previous research carried out in Nigeria demonstrated that only 3 genes (hly A, iap, and prf A) among five screened virulence-associated genes were detected in (9/36) L. monocytogenes isolates (Usman et al., 2016).

 

CONCLUSION

 

The prevalence of L. monocytogenes in the minced meat, poultry meat, tilapia fish, and raw milk samples cannot be ignored and we ought not to disregard the danger of listeriosis, because of the consuming of these foods. Moreover, simultaneous recognition of multiple virulence genes in a single assay is desired as it will be helpful for enormous scale investigations aimed at the detection of pathogenic listeria strains.

 

CONFLICT OF INTEREST

 

The authors declare that no conflict of interest exists.


ACKNOWLEDGEMENTS

 

This work was supported by Animal Health Research Institute, Zagazig, Egypt and Animal Health Research Institute, Dokki, Egypt.

 

AUTHORS CONTRIBUTION

 

All authors contributed equally in this work.

 

REFERENCE

 

  • Abdellrazeq G, Kamar A, ElHoushy S (2014). Molecular Characterization of Listeria species Isolated from Frozen Fish. Alexandria J. Vet. Sci. 40: 1. https://doi.org/10.5455/ajvs.45443
  • Amajoud N, Leclercq A, Soriano JM (2018). Prevalence of Listeria spp. and characterization of Listeria monocytogenes isolated from food products in Tetouan, Morocco. Food Contr. 84: 436-441. https://doi.org/10.1016/j.foodcont.2017.08.023
  • Aurora R, Prakash A, Prakash S (2008). Comparison of PI-PLC based assays and PCR along with in vivo pathogenicity tests for rapid detection of pathogenic Listeria monocytogenes. Food Contr. 19: 641–647. https://doi.org/10.1016/j.foodcont.2007.07.002
  • Bryman A, Cramer D (2002). Quantitative data analysis with SPSS Release 10 for Windows: a guide for social scientists. Routledge. https://doi.org/10.4324/9780203471548
  • Camejo A, Carvalho F, Reis O (2011). The arsenal of virulence factors deployed by Listeria monocytogenes to promote its cell infection cycle. Virulence. 2: 379–394. https://doi.org/10.4161/viru.2.5.17703
  • Chen Y, Knabel SJ (2007). Multiplex PCR for simultaneous detection of bacteria of the genus Listeria, Listeria monocytogenes, and major serotypes and epidemic clones of L. monocytogenes. Appl. Environ. Microbiol. 73: 6299–6304. https://doi.org/10.1128/AEM.00961-07
  • Churchill RLT, Lee H, Hall JC (2006). Detection of Listeria monocytogenes and the toxin listeriolysin O in food. J. Microbiol. Methods. 64: 141–170. https://doi.org/10.1016/j.mimet.2005.10.007
  • Claeys WL, Cardoen S, Daube G (2013). Raw or heated cow milk consumption: Review of risks and benefits. Food Contr. 31: 251–262. https://doi.org/10.1016/j.foodcont.2012.09.035
  • Clayton EM, Hill C, Cotter PD, Ross RP (2011). Real-time PCR assay to differentiate listeriolysin S-positive and-negative strains of Listeria monocytogenes. Appl. Environ. Microbiol. 77: 163–171. https://doi.org/10.1128/AEM.01673-10
  • Coroneo V, Carraro V, Aissani N (2016). Detection of Virulence Genes and Growth Potential in Listeria monocytogenes Strains Isolated from Ricotta Salata Cheese. J. Food Sci. 81: M114–M120. https://doi.org/10.1111/1750-3841.13173
  • De Reu K, Grijspeerdt K, Herman L (2004). A Belgian survey of hygiene indicator bacteria and pathogenic bacteria in raw milk and direct marketing of raw milk farm products. J. food Saf. 24: 17–36. https://doi.org/10.1111/j.1745-4565.2004.tb00373.x
  • Di Ciccio P, Meloni D, Festino AR (2012). Longitudinal study on the sources of Listeria monocytogenes contamination in cold-smoked salmon and its processing environment in Italy. Int. J. Food Microbiol. 158: 79–84. https://doi.org/10.1016/j.ijfoodmicro.2012.06.016
  • Doumith M, Buchrieser C, Glaser P, Jacquet C, Martin P (2004). Differentiation of the Major Listeria monocytogenes Serovars by Multiplex PCR. J. Clin. Microbiol. 42: 3819–3822. https://doi.org/10.1128/JCM.42.8.3819-3822.2004
  • Edris AM, Amany MS, Michael AF (2014). Incidence of Listeria monocytogenes in fresh tilapia nilotica fish. Benha Vet. Med. J. 26 (1): 120-126.
  • El Marnissi B, Bennani L, Cohen N, Lalami AEO, Belkhou R (2013). Presence of Listeria monocytogenes in raw milk and traditional dairy products marketed in the north-central region of Morocco. African J. Food Sci. 7: 87–91. https://doi.org/10.5897/AJFS2013.0992
  • Ertacs HB, cSeker E (2005). Isolation of Listeria monocytogenes from fish intestines and RAPD analysis. Turkish J. Vet. Anim. Sci. 29:1007–1011.
  • Gebretsadik S, Kassa T, Alemayehu H, Huruy K, Kebede N (2011). Isolation and characterization of Listeria monocytogenes and other Listeria species in foods of animal origin in Addis Ababa, Ethiopia. J. Infect. Public Health 4: 22–29. https://doi.org/10.1016/j.jiph.2010.10.002
  • Gelbíčová T, Koláčková I, Karpíšková R (2015). Genotyping and virulence factors of Listeria monocytogenes in terms of food safety. J. Food Nutr. Res. 54: 79–84.
  • Gray MJ, Freitag NE, Boor KJ (2006). How the bacterial pathogen Listeria monocytogenes mediates the switch from environmental Dr. Jekyll to pathogenic Mr. Hyde. Infect. Immun. 74: 2505–2512. https://doi.org/10.1128/IAI.74.5.2505-2512.2006
  • Gudbjörnsdóttir B, Suihko ML, Gustavsson P (2004). The incidence of Listeria monocytogenes in meat, poultry and seafood plants in the Nordic countries. Food Microbiol. 21: 217–225. https://doi.org/10.1016/S0740-0020(03)00012-1
  • Haj Hosseini A, Sharifan A, Tabatabaee A (2014). Isolation of Listeria monocytogenes from meat and dairy products. J. Med. Microbiol. Infect. Dis. 2: 159–162.
  • Henriques AR, Barreto AS, Fraqueza MJ (1998). Detection of virulence-associated genes in Listeria monocytogenes isolates from Portuguese ready-to-eat meat-based food industry. Appl. Environ. Microbiol. 61:817–819.
  • Hitchins A. (2003). Bacteriological analytical manual, chapter 10. Food and Drug Administration. [Online.] http://www.cfsan. fda.gov/ebam/bam10.html.
  • Huehn S, La Ragione RM, Anjum M, Saunders M, Woodward MJ, Bunge C, Helmuth R, Hauser E, Guerra B, Beutlich J (2010). Virulotyping and antimicrobial resistance typing of Salmonella enterica serovars relevant to human health in Europe. Foodborne Pathog. Dis. 7: 523–535. https://doi.org/10.1089/fpd.2009.0447
  • Ismaiel AAR, Ali AES, Enan G (2014). Incidence of Listeria in Egyptian meat and dairy samples. Food Sci. Biotechnol. 23: 179–185. https://doi.org/10.1007/s10068-014-0024-5
  • ISO 11290-1: 1996/Amd 1 (2004). International Organization for Standardization. Geneva, Switzerland.
  • Jadhav S, Bhave M, Palombo EA (2012). Methods used for the detection and subtyping of Listeria monocytogenes. J. Microbiol. Methods 88: 327–341. https://doi.org/10.1016/j.mimet.2012.01.002
  • Kamar AM, Torky HA, El-houshy SM (2014). Phenotypic and Genotypic characterization of Listeria monocytogenes isolated from different sources. 1–19.
  • Kathariou S (2002). Listeria monocytogenes virulence and pathogenicity, a food safety perspective. J. Food Prot. 65: 1811–1829. https://doi.org/10.4315/0362-028X-65.11.1811
  • Kérouanton A, Marault M, Petit L, Grout J, Dao TT, Brisabois A (2010). Evaluation of a multiplex PCR assay as an alternative method for Listeria monocytogenes serotyping. J. Microbiol. Methods 80: 134–137. https://doi.org/10.1016/j.mimet.2009.11.008
  • Kersting AL, Medeiros LC, LeJeune JT (2010). Differences in Listeria monocytogenes contamination of rural Ohio residences with and without livestock. Foodborne Pathog. Dis. 7: 57–62. https://doi.org/10.1089/fpd.2009.0318
  • Kwarteng JO, Wuni A, Akabanda F, Jespersen L (2018). Prevalence and Characteristics of Listeria monocytogenes Isolates in Raw Milk , Heated Milk and Nunu, a Spontaneously Fermented Milk Beverage, in Ghana. Beverages. 4: 40. https://doi.org/10.3390/beverages4020040
  • Liu D, Lawrence ML, Ainsworth AJ, Austin FW (2007a). Toward an improved laboratory definition of Listeria monocytogenes virulence. Int. J. Food Microbiol. 118: 101–115. https://doi.org/10.1016/j.ijfoodmicro.2007.07.045
  • Liu D, Lawrence ML, Austin FW, Ainsworth AJ (2007b). A multiplex PCR for species-and virulence-specific determination of Listeria monocytogenes. J. Microbiol. Methods 71: 133–140. https://doi.org/10.1016/j.mimet.2007.08.007
  • Mohamed Y, Reda WW, Abdel-Moein K (2016). Prevalence and phylogenetic characterization of Listeria monocytogenes isolated from processed meat marketed in Egypt. J. Genet. Eng. Biotechnol. 14: 119–123. https://doi.org/10.1016/j.jgeb.2016.06.001
  • Molla B, Yilma R, Alemayehu D (2004). Listeria monocytogenes and other Listeria species in retail meat and milk products in Addis Ababa, Ethiopia. Ethiop. J. Heal. Dev. 18: 208–212. https://doi.org/10.4314/ejhd.v18i3.9962
  • Momtaz H, Yadollahi S (2013). Molecular characterization of Listeria monocytogenes isolated from fresh seafood samples in Iran. Diagn. Pathol. 8: 149. https://doi.org/10.1186/1746-1596-8-149
  • Monteiro LRL de, Mesquita AJ de, André MCDPB, Cardoso JL (2013). Molecular characterization of Listeria monocytogenes isolated from animal products in a city of Northern Brazil. Ciência. Rural. 43: 1443–1448. https://doi.org/10.1590/S0103-84782013000800016
  • Nayak DN, Savalia C V, Kalyani IH, Kumar R, Kshirsagar DP (2015). Isolation, identification, and characterization of Listeria spp. from various animal origin foods. Vet. World. 8: 695–701. https://doi.org/10.14202/vetworld.2015.695-701
  • Piana A, Are R, Orrù M, Franca Saba, Marco Dettori, Ivana Maida, Giovanni Sotgiu, Claudia Rais, MariaStella Mura (2005). Listeria monocytogenes meningoencephalitis: molecular methods for diagnosis and for monitoring the response to chemotherapy. Ital. J. Public Health. 2: 29-34.
  • Purdue LM, Jamieson RC, Yost CK, Hansen LT (2015). Comparison of the prevalences and diversities of Listeria species and Listeria monocytogenes in an urban and a rural agricultural watershed. Appl. Environ. Microbiol. 81: 3812–3822. https://doi.org/10.1128/AEM.00416-15
  • Rawool DB, Malik SVS, Shakuntala I (2007). Detection of multiple virulence-associated genes in Listeria monocytogenes isolated from bovine mastitis cases. Int. J. Food Microbiol. 113: 201–207. https://doi.org/10.1016/j.ijfoodmicro.2006.06.029
  • Sakaridis I, Soultos N, Iossifidou E, Papa A, Ambrosiadis I, Koidis P (2011). Prevalence and antimicrobial resistance of Listeria monocytogenes isolated in chicken slaughterhouses in northern Greece. J. Food Prot. 74: 1017–1021. https://doi.org/10.4315/0362-028X.JFP-10-545
  • Seyoum ET, Woldetsadik DA, Mekonen TK, Gezahegn HA, Wondwossen A (2015). Prevalence of Listeria monocytogenes in raw bovine milk and milk products from central highlands of Ethiopia. J. Infect. Dev. Ctries. 9(11): 1204-1209. https://doi.org/10.3855/jidc.6211
  • Shen J, Rump L, Zhang Y, Chen Y, Wang X, Meng J (2013). Molecular subtyping and virulence gene analysis of Listeria monocytogenes isolates from food. Food Microbiol. 35: 58–64.https://doi.org/10.1016/j.fm.2013.02.014
  • Soni KA, Nannapaneni R, Hagens S (2010). Reduction of Listeria monocytogenes on the surface of fresh channel catfish fillets by bacteriophage Listex P100. Foodborne Pathog. Dis. 7: 427–434. https://doi.org/10.1089/fpd.2009.0432
  • Usman UB, Kwaga JKP, Kabir J (2016). Molecular Characterization and Phylogenetic Analysis of Listeria monocytogenes Isolated from Milk and Milk Products in Kaduna, Nigeria. Can. J. Infect. Dis. Med. Microbiol. 2016: 1–7. https://doi.org/10.1155/2016/4313827
  • Vasconcelos RM de, Almeida AECC de, Hofer E (2008). Multiplex-PCR serotyping of Listeria monocytogenes isolated from human clinical specimens. Mem. Inst. Oswaldo. Cruz. 103: 836–838. https://doi.org/10.1590/S0074-02762008000800016
  • Vázquez-Boland JA, Kuhn M, Berche P (2001). Listeria pathogenesis and molecular virulence determinants. Clin. Microbiol. Rev. 14: 584–640. https://doi.org/10.1128/CMR.14.3.584-640.2001
  • Wu S, Wu Q, Zhang J, Chen M, Yan ZA, Hu H (2015). Listeria monocytogenes Prevalence and Characteristics in Retail Raw Foods in China. PLoS One, 10 (8): 1–16. https://doi.org/10.1371/journal.pone.0136682
  • Zeinali T, Jamshidi A, Bassami M, Rad M (2017). Isolation and identification of Listeria spp. in chicken carcasses marketed in northeast of Iran. Int. Food Res. J. 24: 881–887.
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    Vol. 12, Iss. 11, pp. 2062-2300

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