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Separation of Enzymes from their Aqueous System by using Novel Concept of Unidirectional Freezing

Separation of Enzymes from their Aqueous System by using Novel Concept of Unidirectional Freezing

Muhammad Waheed Mushtaq1,*, Muhammad Waqar Younas2, Jamil Anwar2, Syed Mustansar Abbas3 and Shahid Bashir3

1Institute of Chemistry, University of the Punjab, Lahore - 54590
2Department of Chemistry, Government College of Science, Wahdat Road, Lahore
3Department of Chemistry, Faculty of Science, University of Malaya, Kuala Lumpur 50603, Malaysia

*      Corresponding author: waheedjaami@gmail.com

 

Fig. 1.

Effect of radial freezing over centralization of pectinase enzyme.

Fig. 2.

Comparison of centralization efficiency of three enzymes (pectinase, cellulase and α-amylase) after applying UFT.

Fig. 3.

Relative quantitative comparison of centralization of enzymes.

Fig. 4.

Distance travelled by amylase and its relative concentration after applying UFT.

Fig. 5.

 Activity of extracted α-amylase over grey (A) and treated (B) fabric.

Fig. 6.

Digestion test for cellulose.

Fig. 7.

Biopolishing test for our extracted enzyme cellulase, sample-1 (A) and standard sample (B).

Fig. 8.

Concentration of pectinase in various sections of glass bowl after applying UFT.

Pakistan Journal of Zoology

December

Vol. 51, Iss. 6, Pages 1999-2399

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