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RNA Interference Mediated Knockdown of ATP Binding Cassette Subfamily A Member 1 Decreases the Triglyceride Content of Bovine Mammary Epithelial Cells

RNA Interference Mediated Knockdown of ATP Binding Cassette Subfamily A Member 1 Decreases the Triglyceride Content of Bovine Mammary Epithelial Cells

Ping Jiang1 , Zhihui Zhao1, 2, Xiaohui Li2, Mengyan Wang2, Lixin Xia2, Yang Cao3, Runjun Yang2 and Xibi Fang2*

1Agricultural College, Guangdong Ocean University, Zhanjiang, 524088, People’s Republic of China. 
2College of Animal Science, Jilin University, Changchun 130062, People’s Republic of China. 
3Branch of Animal Husbandry, Jilin Academy of Agricultural Sciences, Changchun 130033, P.R. China.

Ping Jiang and Zhihui Zhao contributed equally to this work

*      Corresponding author: fangxibi@jlu.edu.cn

 

Fig. 1.

ABCA1 RNAi vector and the negative control vector were transfected into BMECs; A, Cells transfected with ABCA1-shRNA; B, cells transfected with negative control vector.

Fig. 2.

 A, ABCA1 mRNA expression levels in BMECs; B, Comparison of the TG content of cells transfected with RNAi vector or negative control vector; C, ABCA1 protein expression levels using a grey value analyser to calculate grey value ratios (target protein content/β-actin content).

Fig. 3.

The protein-protein interaction between ABCA1 gene and related fatty acid metabolism pathway genes was obtained by STRING.

Pakistan Journal of Zoology

December

Vol. 51, Iss. 6, Pages 1999-2399

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