RNA Interference Mediated Knockdown of ATP Binding Cassette Subfamily A Member 1 Decreases the Triglyceride Content of Bovine Mammary Epithelial Cells
RNA Interference Mediated Knockdown of ATP Binding Cassette Subfamily A Member 1 Decreases the Triglyceride Content of Bovine Mammary Epithelial Cells
Ping Jiang1 , Zhihui Zhao1, 2, Xiaohui Li2, Mengyan Wang2, Lixin Xia2, Yang Cao3, Runjun Yang2 and Xibi Fang2*
ABCA1 RNAi vector and the negative control vector were transfected into BMECs; A, Cells transfected with ABCA1-shRNA; B, cells transfected with negative control vector.
A, ABCA1 mRNA expression levels in BMECs; B, Comparison of the TG content of cells transfected with RNAi vector or negative control vector; C, ABCA1 protein expression levels using a grey value analyser to calculate grey value ratios (target protein content/β-actin content).
The protein-protein interaction between ABCA1 gene and related fatty acid metabolism pathway genes was obtained by STRING.