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Prevalence and Molecular Detection of Dengue Virus in 2013 Outbreak in KPK and Punjab, Pakistan

Prevalence and Molecular Detection of Dengue Virus in 2013 Outbreak in KPK and Punjab, Pakistan

Muhammad Shahid1, Iram Amin1,*, Samia Afzal1, Zareen Fatima1, Sadia Zahid1, Usman Ashraf1 and Muhammad Idrees2

1Division of Molecular Virology, National Centre of Excellence in Molecular Biology (CEMB), University of the Punjab, Lahore, Pakistan
2Centre of Applied Molecular Biology (CAMB), University of the Punjab, Lahore, Pakistan

*        Corresponding author: Iram_mali2005@yahoo.com

ABSTRACT

Dengue Virus is transmitted to humans by Aedes aegypti and Aedes albopictus having five serotypes 1-5 and infecting about 100 million people each year. To address dengue outbreak 2013 in Pakistan we aimed to conduct a comprehensive study at molecular level to determine the main causative serotype of 2013 DENV outbreak in Pakistan. Overall 703 serologically positive suspected patients from different major health centers of Pakistan were registered in present study of which 214 were females and 489 were male. Overall weighted prevalence of PCR positivity was 38% (268). Of the total 268 PCR positive subjects, 0.74%, 56.71%, 15.67% were positive for serotype 1, 2 and 3 respectively. Only 72 suspected patients had concurrent infection with serotype 2 and 3. Higher incidence of RNA positivity by PCR was found in males (71%) as compared to the females (29%). The higher incidence of PCR positivity was found in age group of 16-25 rather than teenage (≤16) and older age group (≥50). From the result of this febrile illness study we concluded that males and young people are more susceptible to Dengue virus infection. Moreover serotype-2 was the main causative serotype of the 2013 outbreak of dengue fever.
 

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Pakistan Journal of Zoology

October

Vol. 51, Iss. 5, Pages 1599-1997

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