Molecular Studies on Some Virulent and Multi-Drug Resistant Cattle Klebsiella Strains and their Heamatobiochemical Impacts
Molecular Studies on Some Virulent and Multi-Drug Resistant Cattle Klebsiella Strains and their Heamatobiochemical Impacts
Ghada A. Ibrahim1*, Mohamed Sayed Helal1, Nahed Abd Elhafeeze Kamoura2, Mohamed Samir3,4, Amira Mohamed Mazid5, Mohamed F.M. Farag6
ABSTRACT
Multidrug resistant Klebsiella spp. (MDR) and the extended-spectrum đť›˝-lactamases (ESBL) producing strains are one of the dominantly pathogenic bacteria that involved in bovine respiratory infections. Its spreading problem is growing rapidly especially it could combine resistance with high-level of virulence traits that make it is more difficult to be treated. This study provides insight into the respiratory infections by Klebsiella spp. and its MDR profile in bovine cases, as well as the potential target genes that were involved in the links between virulence determinants and MDR. The bacterial examination of 200 nasal swabs and 30 lung tissue samples (taken from 30 emergency slaughtered cattle) indicated that Klebsiella spp. was recovered in 55/230 (24%) of all samples with a high proportion of K. pneumoniae (18.7%). In-vitro antibiogram exhibited a wide MDR phenotype. The virulence genes (fimA, aerobactin, rmpA and magA) were exhibited in 90%, 85%, 90% and 80% of the tested isolates, respectively. Moreover, ESBL-resistant markers (blaCTX-M, blaTEM and blaSHV) were found in 100%, 85% and 80% of the same isolates, respectively. A significant positive correlation (R = 0.7) was reported between aerobactine and magA virulence genes and blaTEM and blaSHV ESBL resistant genes, respectively. In addition, the hemato-biochemical analysis of 100 blood samples (50 from naturally infected and 50 from apparently healthy cattle) revealed significant decreases in the haemobiogram parameters levels meanwhile, leucogram picture total proteins, globulins, urea, creatinine levels were significantly increased in klebsiella-infected animals. An alarming increase in MDR and ESBL klebsiella bovine infections necessitates a controlled use of antibiotics in cattle farms and warrants sustainable monitoring of antibiotic emergence events and further studies for their genetic phenotypic interrelation. Moreover, the haemato-biochemical alterations in the klebsiella infected cattle could be act as biomarkers for bacterial infections.
Keywords | Klebsiella spp, ESBL, Heamato-biochemical; antibiotics, MDR, Virulence, Resistant, Hemobiogram, Leucogram, Cattle farms
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March 2023
Vol. 11, Iss. 3, Pages 355-516
