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Incidence of Diarrheagenic Escherichia coli Pathotypes in Children Suffering from Diarrhea in Tertiary Care Hospitals, Quetta, Pakistan

Incidence of Diarrheagenic Escherichia coli Pathotypes in Children Suffering from Diarrhea in Tertiary Care Hospitals, Quetta, Pakistan

Zil-e-Huma1, 2, Abdul Malik Tareen3, Kaleem Ullah3, Tauseef M Asmat4,Abdul Samad4Asim Iqbal2, Mohammad Zahid Mustafa3*,Irshad Ahmad5 and Sadeeq ur Rahman6

1Department of Zoology, Sardar Bahadur Khan Women’s University, Quetta
2Department of Zoology, University of Balochistan, Quetta
3Department of Microbiology, University of Balochistan, Quetta
4Centre of Advanced Studies in Vaccinology and Biotechnology (CASVAB), University of Balochistan, Quetta
5Institue of Basic Medical Sciences, Khyber Medical University, Peshawar
6Collge of Veterinary Sciences and Animal Husbandry, Abdul Wali Khan University, Mardan, KP

*  Corresponding author: sadeeq@awkum.edu.pk

 

ABSTRACT

Diarrhea is one of the leading public health problems in under developed countries. In Pakistan, diarrheal cases are treated empirically and, rarely, specific investigations are made. This study was designed to identify the prevalent strains of Escherichia coli among children of less than five years of age in Quetta, Balochistan, Pakistan. A total of 200 samples were collected from children suffering with diarrhea. E. coli was isolated using standard biological procedures and confirmed by colonial characteristics, biochemical profile and specie specific PCR. Multiplex PCR was applied to differentiate among the five main diarrheagenic E. coli pathotypes (enteropathogenic E. coli (EPEC), enteroaggregative E. coli (EAEC), enterotoxigenic E. coli (ETEC), enteroinvasive E. coli (EIEC), and enterohemorrhagic E. coli (EHEC). Results show that the virulent EAEC strain of E. coli was found to be the most prevalent (35%), while EPEC was identified in 15%, followed by EHEC (8%). Notably, none of the samples was found positive for EIEC. Moreover, ETEC was identified in 11% cases of co-infection. Results showed that children with less than two years of age were affected significantly. However, gender had no significant effect on acquiring diarrhea. In conclusion, pathogen spectrum of pathogenic E. coli is EAEC strain. Children under one year of age were affected significantly, and no significant effect was observed when data was stratified on gender base. Further studies are required to investigate the other pathological agents and control parameters should be strengthened to minimize the risks. 
 

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Pakistan Journal of Zoology

October

Vol. 51, Iss. 5, Pages 1599-1997

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