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Geniposide Can Rescue the Erythropoiesis Inhibition Caused by Chemotherapy Drug 5-Fu

Geniposide Can Rescue the Erythropoiesis Inhibition Caused by Chemotherapy Drug 5-Fu

Hong-mei Gao, Wen-long Su, Gui-bin Tao, Han-yang Li, Wen-zheng Cao and Zhi-dong Qiu*

Changchun University of Chinese Medicine, Changchun 130117, Jilin, China.

*      Corresponding author: qzdcczy@163.com

 

Fig. 1.

Bar chart for the geniposide effects on erythroid differentiation. (A), BFU-E colonies assayed quantification from BM and PB derived CD34+ cells after 14 days incubation, CD34+ cells cultured in a methylcellulose-based culture supplemeted with SCF, EPO, and IL-3, including control group (absence Geniposide) or experiment group (pesence of Geniposide , 50 and 100 ug/ml); (B), flow cytometric analyses of cells expression CD71 and Ter119 generated from BM derived CD34+ cells incubated for 7 days in serum-free liquid culture system (IMDM-based media with SCF and EPO) in the control group (absence Geniposide) or experiment group (presence of Geniposide, 100ug/mL); (C), flow cytometry histograms of Ter119 expression of erythroid culture after 14 days in liquid culture in the control group (absence Geniposide) or experiment group (presence of Geniposide, 100ug/mL).

Fig. 2.

Evaluation of geniposide condition in mice. (A), schematic representation of the mouse experient design for different time point including control group, 5-Fu treatment group and 5-Fu + Geniposide group; (B, C), parameters of PB were summarized from control group, 5-Fu treatment group and 5-Fu + Geniposide group mice: RBC count (B) and Hb (C).

Fig. 3.

Evaluation of erythroid cells, LSK / LK cells in mouse BM and spleen. (A), flow cytometry analysis erythroid cells population of BM and spleen cells of representative in control group, 5-Fu treatment group and 5-Fu + Geniposide group mice; (B), quantitation of the percent of erythroid cells in total BM cells of each group mice; (C), flow cytometric analysis of LSK and LK compartments in BM of control group, 5-Fu treatment group and 5-Fu + Geniposide group mice; (D), quantitation of the percent of LSK, LK, CMP, GMP, and MEP cells in total BM cells of each group mice; (E), spleen size compare for including control group, 5-Fu treatment group and 5-Fu + Geniposide group; (F), H&E staining of paraffin-embedded sections of BM and spleen for control group, 5-Fu treatment group and 5-Fu + Geniposide group.

Fig. 4.

Effects of geniposide after treatment with 5-Fu. Western blot assay showed in the BM that, the p-Akt expressions had lower expressions in 5-Fu treatment group compared with control group. The low expressions rescued by add Geniposide in 5-Fu + Geniposide group. In spleen, there is no significant difference.

Fig. 5.

The hypothesis schematic representation of the potential mechanisms underlying geniposide effects on cell survival.

Pakistan Journal of Zoology

June

Vol. 52, Iss. 3, Pages 825-1224

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