Green tea extract is plant–dervied natural antioxidant. The objective of this study was to explore the antioxidative effect of green tea extract on the cryopreserved bull semen. Experiments were carried out on Achai breeding bulls of similar age at the Semen Production Unit, Harichand, District Charsadda, Khyber Pakhtunkhwa. Collection of semen was done using artificial vagina (42°C) for three weeks. Semen was extended in egg yolk extender at various inclusion levels (0.0%, 0.25%, 0.5%, 0.75 and 1.0%) of green tea extract. Testing was consummated for quality characteristics of post-thawed spermatozoa such as motility, viability and plasma membrane integrity. The data was statistically analyzed with one-way analysis of variance using SPSS. The highest significant (P<0.05) response was obtained in extenders containing 0.75% green tea extract for all studied parameters. Based on the findings of the current study, it can be concluded that supplementation of green tea extract in semen extender showed significant response on the post-thawed spermatozoa motility, viability and membrane integrity of Achai bull.