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Comparing the Efficacy of Multi-Source Serum for in vitro Culture of Equine Achilles Tendon Fibroblasts

Comparing the Efficacy of Multi-Source Serum for in vitro Culture of Equine Achilles Tendon Fibroblasts

Somia Shehzadi1, Sana Javaid Awan2, Maryam Javed1*, Asif Nadeem1 and Tahir Yaqub3

1Institute of Biochemistry and Biotechnology, University of Veterinary and Animal Sciences, Lahore, Pakistan
2 Institute of Molecular Biology and Biotechnology, University of Lahore, Lahore, Pakistan
3Department of Microbiology, University of Veterinary and Animal Sciences, Lahore, Pakistan

*      Corresponding author: maryam.javed@uvas.edu.pk

Fig. 1.

A, All cells at passage 4 showing flattened and spindle-shaped morphology, A) cultured with FBS, 100X, B) culture with AES, 100X (AES) and C) cultured with ABS, 100X (ABS). No detachment was observed under any serum group. B, Viability of ATF’s assessed by trypan blue stain method showed no significant difference and data was expressed as Mean±SEM by keeping level of significance ≤0.05.

Fig. 2.

A, Expression of Collagen type1 (Col 1) on ATFs after supplementation with 10% FBS (A1-A3), 10%AES (B1-B3) and 10%ABS (C1-C3). Cells were stained with anti-type1 collagen and FITC conjugated secondary antibody whereas DAPI was used to stain the nucleus (X200). B, Expression of Collagen type1 (Col 1) in ATFs cultured with 10% FBS, AES and ABS sera. Scattered dot plot shows that there is same level of Col type1 expression all groups. Data was expressed as Mean ±SEM and level of significance was < 0.05.

Pakistan Journal of Zoology

April

Vol. 53, Iss. 2, Pages 401-800

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