Join Us  |   Site Map
Submit or Track your Manuscript LOG-IN

Effects of Letrozole on Gonad Differentiation of Carp (Cyprinus carpio)

Effects of Letrozole on Gonad Differentiation of Carp (Cyprinus carpio)

Fang Wang, Yong-Fang Jia, Po Wang, Qi-Yan Du and Zhong-Jie Chang*

 

College of Life Science, Henan Normal University, Xinxiang, Henan 453007, People’s Republic of China

*        Corresponding author: 13837331530@163.com

Fig 1

Histological structure of the gonads and brains of juvenile carp from control and the groups exposed to LET for 15d. a, Ovary from control groups showing abundance of large, closely arranged, uniformly developed primary oocytes; b, Ovary from LET(625μg/L)-treated groups showing increased prevalence of smaller early differentiated oocytes and enlarged ovarian plate space; c, Testis from control groups; d, from LET(625μg/L)-treated groups showing increased spermatogonial cells; e, brain from control male; f, brain from LET(625μg/L)-treated male; g, brain from control female; h, brain from LET(625μg/L)-treated female. PO, primary oocytes.

Fig 2

The expression of Cyp19A in the brain and gonads of juvenile carp treated with 0, 5, 25, 125, 625μg/L letrozole for 15 d. Data are presented as means ± S.E. (n=5) and normalized against the mRNA expression of β-actin. The asterisk symbols denote significant differences compared with the control group: *p< 0.05 and **p< 0.01.

Fig 3

The expression of Cyp19B in the brain and gonads of juvenile carp treated with 0, 5, 25, 125, 625μg/L letrozole for 15 d. Data are presented as means ± S.E. (n=5) and normalized against the mRNA expression of β-actin. The asterisk symbols denote significant differences compared with the control group: *p< 0.05 and **p< 0.01.

Fig 4

The expression of Foxl2 in the brain and gonads of juvenile carp treated with 0, 5, 25, 125, 625μg/L letrozole for 15 d. Data are presented as means ± S.E. (n=5) and normalized against the mRNA expression of β-actin. The asterisk symbols denote significant differences compared with the control group: *p< 0.05 and **p< 0.01.

Fig 5

The expression of Dax in the brain and gonads of juvenile carp treated with 0, 5, 25, 125, 625μg/L letrozole for 15 d. Data are presented as means ± S.E. (n=5) and normalized against the mRNA expression of β-actin. The asterisk symbols denote significant differences compared with the control group: *p< 0.05 and **p< 0.01.

Fig 6

The expression of Erα in the brain and gonads of juvenile carp treated with 0, 5, 25, 125, 625μg/L letrozole for 15 d. Data are presented as means ± S.E. (n=5) and normalized against the mRNA expression of β-actin. The asterisk symbols denote significant differences compared with the control group: *p< 0.05 and **p< 0.01.

Fig 7

 The expression of Arα in the brain and gonads of juvenile carp treated with 0, 5, 25, 125, 625μg/L letrozole for 15 d. Data are presented as means ± S.E. (n=5) and normalized against the mRNA expression of β-actin. The asterisk symbols denote significant differences compared with the control group: *p< 0.05 and **p< 0.01.

Fig 8

 The expression of Dmrt1 in the brain and gonads of juvenile carp treated with 0, 5, 25, 125, 625 μg/L letrozole for 15 d. Data are presented as means ± S.E. (n=5) and normalized against the mRNA expression of β-actin. The asterisk symbols denote significant differences compared with the control group: *p< 0.05 and **p< 0.01.

Pakistan Journal of Zoology (Associated Journals)

October

Vol. 49, Iss. 5, Pages 1547-1936

Featuring

Click here for more

Subscribe Today

Receive free updates on new articles, opportunities and benefits


Subscribe Unsubscribe

Commons Attribution License

This license permits unrestricted use, distribution and reproduction in any medium, provided the original work is properly cited.

Creative Commons License
Follow ResearchersLinks