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Abattoir based Sero-Survey of Mycobacterium avium Subspecies Paratuberculosis in Bovines in District Faisalabad-Pakistan

Abattoir based Sero-Survey of Mycobacterium avium Subspecies Paratuberculosis in Bovines in District Faisalabad-Pakistan

Rais Ahmed1,2,*, Muhammad Khalid Mansoor1,2, Iftikhar Hussain1, Muhammad Saqib3, Muhammad Hammad Hussain4, Amjad Islam Aqib5, Haleema Sadia6, Javed Muhammad7, Asma Irshad8, Kashif Prince5, Muhammad Zain Saleem9 and Abdul Whab Manzoor10

1*Institute of Microbiology, University of Agriculture, Faisalabad-38000
2Department of Microbiology, Cholistan University of Veterinary and Animal Sciences, Bahawalpur
3Department of Clinical Medicine and Surgery, University of Agriculture, Faisalabad
4Animal Health Research Center, Ministry of Agriculture and Fisheries, Muscat, Oman
5Department of Medicine, Cholistan University of Veterinary and Animal Sciences, Bahawalpur
6Department of Biotechnology, Balochistan University of Information Technology, Engineering and Management Sciences, Quetta-87300
7Department of Microbiology, University of Swabi, Anbar
8National Centre of Excellence in Molecular Biology, University of the Punjab, Lahore
9Department of Pathology, University of Veterinary and Animal Sciences, Lahore
10Veterinary Research Institute, Zarrar Shaheed Road, Lahore Cantt. 

*  Corresponding author: dr.raisahmad2068@gmail.com

0030-9923/2020/0115-0001 $ 9.00/0
Copyright 2020 Zoological Society of Pakistan

 

ABSTRACT

Bovine paratuberculosis is a chronic disease of cattle and buffaloes, causing progressive weight loss, persistent diarrhea and finally death. Due to zoonotic nature of the disease, workers in slaughterhouses are at a high risk of infection. In this study, the status of paratuberculosis was assessed in a slaughterhouse located in district Faisalabad, Pakistan. A total of 455 blood samples were collected from slaughtered cattle and buffaloes at random and then tested by a commercially available ELISA kit for the detection of antibodies against Mycobacterium avium subsp. paratuberculosis (MAP). Data were analyzed using chi square for ELISA and odds ratio for finding association of species, gender, body weight, age and body condition score (BCS) with prevalence of antibodies against MAP. Seropositivity against MAP was significantly (P<0.05) detected. Male and female animals were equally susceptible to the disease (OR: 0.908, 95% CI= 0.6111-1.350). Age groups were not found associated with chances of being seropositive. Seropositivity was significantly (P<0.05) higher in animals under 300kg body weight. Chances of being seropositive were found more in animals belonging to BCS 1 (OR: 13.25, 95%CI=6.29-27.88) and BCS 3 (OR: 4.78, 95%CI=2.36-9.70) than BCS 4 and above. In conclusion, gender, body weight and body condition score of the animals are positively associated with the occurrence of the disease. These data will not only help in screening of animals but will also be instrumental in future for isolation of MAP to make vaccine against paratuberculosis using local isolates.
 

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Pakistan Journal of Zoology

December

Vol. 51, Iss. 6, Pages 1999-2399

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