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A Study on Differentially Expressed Genes in Reserve Mesenchyme of Male and Female Reindeer Antler Tip

A Study on Differentially Expressed Genes in Reserve Mesenchyme of Male and Female Reindeer Antler Tip

Jiancheng Zhai, Li Gao, Yanling Xia and Heping Li*

College of Wildlife Resources, Northeast Forestry University, Harbin 150040, China

Jiancheng Zhai and Li Gao contributed equally to this work. 

*      Corresponding author: lihepinghrb@163.com

Fig 1

Layer identification of unstained proliferative zone in a growing reindeer antler tip. D, dermis; RM, reserve mesenchyme; PC, precartilage; TZ, transitional zone; C, cartilage.

Fig 2

Electrophoresis of RNA of reindeer antler mesenchyme. 1, the male reindeer; 2 and 3, the female reindeer; M, 2000bp DNA marker.

Fig 3

Electrophoresis of partial products of DDRT-PCR. The lanes represent the DDRT-PCR from reserve mesenchyme of male and female reindeer antler tip. The differentially expressed fragments are indicated by arrows.

Fig 4

Electrophoresis of recovered differential fragments. 1 to 5 represented fragments Ci2, Ce1, Ae1, Af1 and Af3, respectively. M, 2000bp DNA marker.

Fig 5

Melt curve of real-time PCR. Yellow, β-actin gene. Green, COL6A3 gene.

Fig 6

Relative quantitative of samples. The 1 and 2 mean the relative expression levels of the reserve mesenchyme of the male and the female reindeer antler tip, respectively.

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Pakistan Journal of Zoology

June

Vol. 50, Iss. 3, Pages 799-1198

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