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A Study on Differentially Expressed Genes in Reserve Mesenchyme of Male and Female Reindeer Antler Tip

A Study on Differentially Expressed Genes in Reserve Mesenchyme of Male and Female Reindeer Antler Tip

Jiancheng Zhai, Li Gao, Yanling Xia and Heping Li*

College of Wildlife Resources, Northeast Forestry University, Harbin 150040, China

Jiancheng Zhai and Li Gao contributed equally to this work. 

*      Corresponding author: lihepinghrb@163.com

Fig 1

Layer identification of unstained proliferative zone in a growing reindeer antler tip. D, dermis; RM, reserve mesenchyme; PC, precartilage; TZ, transitional zone; C, cartilage.

Fig 2

Electrophoresis of RNA of reindeer antler mesenchyme. 1, the male reindeer; 2 and 3, the female reindeer; M, 2000bp DNA marker.

Fig 3

Electrophoresis of partial products of DDRT-PCR. The lanes represent the DDRT-PCR from reserve mesenchyme of male and female reindeer antler tip. The differentially expressed fragments are indicated by arrows.

Fig 4

Electrophoresis of recovered differential fragments. 1 to 5 represented fragments Ci2, Ce1, Ae1, Af1 and Af3, respectively. M, 2000bp DNA marker.

Fig 5

Melt curve of real-time PCR. Yellow, β-actin gene. Green, COL6A3 gene.

Fig 6

Relative quantitative of samples. The 1 and 2 mean the relative expression levels of the reserve mesenchyme of the male and the female reindeer antler tip, respectively.

Pakistan Journal of Zoology (Associated Journals)

April

Vol. 49, Iss. 2, Pages 425-759

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