Search for Antler

A Study on Differentially Expressed Genes in Reserve Mesenchyme of Male and Female Reindeer Antler Tip

Jiancheng Zhai, Li Gao, Yanling Xia and Heping Li*

... which the females carry antlers in Cervidae, and the differences between the antler tips of the two sexes are one of the most interesting scientific mysteries. The objective of this study was to isolate preliminarily differentially expressed genes in antler mesenchyme of both sexes. The genes were detected with three anchored primers and ten random primers using differential display rever...

Molecular Cloning and Expression Analysis of Vps26A Gene from Deer Antler Tip of Different Growth Stages

Yanling Xia^1,2, Heping Li², Yuntao Liang², Jichen Zhao², Binshan Lu² and Di Liu^1,*

...successfully cloned from antler tip of the Sika deer (Cervus nippon hortulorum). The Vps26A cDNA contains an open reading frame of 984bp encoding a polypeptide with 327 amino acids. The deduced molecular mass and isoelectric point of Vps26A protein were 38.2 kDa and 6.13, respectively. Glutamic acid had the largest proportion (10.4%) in the primary structure. Homologous sequence alignment and phylogenetic tree analysis indicated that theVps26A protein o...

An Antimicrobial Cerebroside from the Liposoluble Constituent of Cervus Nippon Antler Velvet Layer

Nina Bao^1,*, Yongguang Yin² and Panpan Wang³

...tituent of cervus nippon antler velvet layer was studied in this paper. Single-factor experiment was used to research the effect of liquid-to-solid ratio, standing time and centrifugation time on the extraction of crude cerebroside. The optimal extraction conditions for the cerebroside as follows: liquid-to-solid ratio 15, standing time 45 min, and centrifugation time 6 min. The extracted cerebroside was purified and identified by using silica gel column and T...

Isolation and Purification of 18KD Protein of Sika Deer Antler Plate and its Antibacterial Activity

Lin Qi, Lu Li, Danyang Chen, Mingxiao Liu, Yan Wu and Wei Hu^*

...l monomeric peptide from antler plate polypeptide (APP-1) of sika deer which inhibited the growth of both Gram-positive and Gram-negative bacteria. The molecular mass and purity of this polypeptide was determined by ultra-performance liquid chromatography (UPLC) and Bruker micOTOF OllQ TOF mass spectrometry, respectively. The full amino-acid sequence of the monomericpeptide was analyzed by sequential Edman degradation using a protein/peptide sequencer. The ant...

Effect of Pilose Antler Peptide on Doxorubicin-induced H9c2 Cells Injury via TGF-β/Smad/ERK Signaling Pathway

Yan Xu¹, Jia Zhou¹, Guangfu Lv², Yuexin Liu¹, Xintong Zhao¹, Xin Li¹, Doudan Ye², Xiaobo Qu^1,* and Xiaowei Huang¹*

...es the effects of pilose antler peptide (PAP) on DOX-induced myocardial injury and related mechanisms through in vitro experiments. Different concentrations of DOX were used to treat H9c2 cells for 6, 12, 24, and 48 h, respectively, to determine the IC₅₀. Different concentrations of PAP were treated to H9c2 cells for 24, 48, and 72 h to determine the effect of PAP on H9c2 cells. The experiment was divided into control group, DOX group, PAP group and...

Differential DNA Methylation of Growth Factors in Antlers of Sika Deer and Reindeer

Jian-Cheng Zhai^1,2, Sheng-Nan Wang^1,3, Qiang-Hui Wang¹, Yan-Ling Xia¹, Wei-Shi Liu¹, Ya-Jie Yin⁴ and He-Ping Li¹*

...ecies in both sexes grow antlers. Many apparent differences exist in antlers of female and male reindeer and sika deer. Insulin like growth factor 1 (IGF1), Keratinocyte growth factor (KGF) and Nerve Growth Factor (NGF) are essential for antler growth. To investigate whether epigenetic regulation of the growth factors is important in growth of sika deer, female and male reindeer

Mitochondrial ATPase 6/8 Genes Based Molecular Diversity and Phylogeny Analysis in Hog Deer (Axis porcinus)

Muhammad Nauman Arif¹, Muhammad Mansha¹* and Tanveer Hussain²

...ue to its meat, skin and antlers, but there are insufficient molecular data, provoking us to explore its genetic variation and phylogenetic analysis using ATPase 6/8 genes of mitochondrial DNA. The blood samples from hog deer were collected and DNA was extracted by using in-house organic methods. PCR was used to amplify a total of 880 bp of overlapping genes, which were then sequenced. Bioedit software was used to align, and edit the sequences, and single nucl...

Protective Mechanism of Velvet Antler Polypeptide on Myocardial Ischemia-Reperfusion Injury in Rats

Ying Chen¹, Chao-Zheng Li², Zhun Yu³, Jia Zhou⁴, Yan Xu⁴, Zhe Lin⁴, He Lin⁴* and Xiao-Wei Huang⁴*

...-align: justify;">Velvet antler polypeptide (VAP) is the active compounds of velvet antler, an animal derived traditional Chinese medicine. In this work, the myocardial ischemia-reperfusion injury (MIRI) model was established to investigate the protective mechanism of VAP on MIRI in rats. Total 90 male Sprague Dawley (SD) rats were divided into the control, model (MIRI), positive (Diltiazem, DLZ, 20 mg/kg), VAP high, medium,...

Mechanism of Action of Velvet Antler Polypeptide in Improving Mild Cognitive Impairment in Rats

Xiao-Wei Huang^1,2, Mei-Li Liu¹, Jin-Ji Wang¹, Yue-Xin Liu³, Zhe Lin¹, Chun-Shu Rong⁴* and Ji-Xiang Ren⁴*

... the mechanism of velvet antler polypeptide (VAP) in improvement of learning and memory function in mild cognitive impairment (MCI) rats model, specifically by investigating the PI3K, AKT and mTOR pathway. The type and content of amino acids (AA) in VAP were determined using pre-column derivatization-high performance liquid chromatography (PD-HPLC). Wistar rats were randomly divided into 5 groups (n=10 per group): control, model, piracetam (PIR), VAPH (300 mg/...

Screening of Transcriptional Differential Genes in Antler After Top Pruning and Expression Characteristics of BVES Gene

Hong Chen^1,2, Jiawei Liu^1,2, Chuan Lin^1,2, Hao Lv^1,2, Jiyun Zhang^1,2, Xiaodong Jia^1,2, Qinghua Gao^1,2 and Chunmei Han^1,2*

...ique mammalian appendage antler is a good model for studying tissue regeneration and its related mechanism. This study explored the mechanism of the rapid growth of regenerated antlers after top pruning damage using transcriptome sequencing technology. A damage repair model was constructed by top pruning treatment on the left antlers of three 10-month-old Tarim red deer, and RNA-seq analys...