Research Article

 

Effect of Protein Rate on the Growth, Feed Efficiency and Composition of Hoplobatrachus Occipitalis (Günther, 1858) Tadpoles’ Carcass in Controlled Medium

 

Théophile Godome1,2*, Dogbè Clément Adjahouinou1, DS. Juste Vital Vodounnou1, Nahoua Issa Ouattara2, Emile Didier Fiogbe1

1Laboratory of Research on Wetlands, Department of Zoology, Faculty of Sciences and Techniques, University of Abomey-Calavi, BP: 526 Cotonou (Benin); 2Laboratory of Hydrobiology, UFR Biosciences, University Félix Houphouet-Boigny, 22 BP: 582 Abidjan 22, (Ivory Coast).

 

Editor | Muhammad Nauman Zahid, Quality Operations Laboratory, University of Veterinary and Animal Sciences, Lahore, Pakistan.

Received | January 02, 2019 Accepted | February 22, 2019; Published | March 10, 2019

*Correspondence | Theophile Godome, Laboratory of Research on Wetlands, Department of Zoology, Faculty of Sciences and Techniques, University of Abomey-Calavi, BP: 526 Cotonou (Benin); Email: [email protected]

Citation | Godome T, Adjahouinou DC, Vodounno (2019). Effect of protein rate on the growth, feed efficiency and composition of Hoplobatrachus occipitalis (Günther, 1858) tadpoles’ carcass in controlled medium. S. Asian J. Life Sci. 7(1): 1-7.

DOI | http://dx.doi.org/10.17582/journal.sajls/2019/7.1.1.7

ISSN | 2311–0589

Copyright © 2019 Godome et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

 

INTRODUCTION

 

Feeding is the most important trouble encountered in commercial frog rearing. Its implementation depends on the three development stages of frog namely tadpoles, bulbous crowfoot and adult frogs (Cairns, 1967; Hirschfeld, 1970). Tadpole is generally fed on Artemia, boiled egg yolk, boiled fish, rice bran or vapour boiled egg (Prapee et al., 1997). The best feed for bulbous crowfoot are tadpoles, fishes and crayfish (Priddy and Culley, 1971) while the feed of adult frogs is preferably insects (Prapee et al., 1997). However, fish pelleted feed are also good for frog growth (Prapee et al., 1997). Considering the worldwide increasing of frog meat demand and the drastic decrease of natural stock, frog rearing has been initiated these latter years (Crumlish and Inglis, 1999; Somsueb and Boonyaratpalin, 2001; Chardonnet et al., 2002; Miles et al., 2003). According to FAO (2006), the part of worldwide frog trade (all species considered) supplied by aquaculture increased from 3% in 1980 to 15% in 2002. Due to the good taste of their meat like that of chicken and their color, frog consumption spread over many countries (Tokur et al., 2008). Importation of frog thigh was estimated to about 8000 tons in 2001 in the European Union (Negroni, 2003). Concerning the United States, they imported 2.232 tons in 2004 and 2.876 tons in 2005 (NOAA, 2005).

 

Table 1: Approximate formulation and composition of experimental diets.

 

Ingredients	Protein rates in experimental diets (%)
	P1 (20%)	P2 (30%)	P3 (40%)	P4 (45%)	P5 (50%)	P6 (60%)
Casein a	7	10.5	21	25	32	44
Fish meal b	13	21	25	28	28	28
Yeast c	14	16	16	16	16	14
Dextrine d	25.5	20	9	6	4.5	2
Glucose e	27.5	21	18.5	15	9	2
Liver oil f	3	2,5	2	2	2	2
Soya oil g	3	3	2.5	2	2.5	2
Vitamin Premix h	3	2.5	2.5	2.5	2.5	2.5
Mineral Premix i	3	2.5	2.5	2.5	2.5	2.5
Caroxylmethylcellulose	1	1	1	1	1	1
Diets composition
Dry matters (DM%)	89.857	89.150	89.407	90.551	90.843	89.825
Ash (%DM)	14.841	15.574	13.686	15.410	14.749	16.054
Crude Protein (%)	21.321	30.105	40.308	45.142	50.249	57.884
Crude lipid (%)	7.406	7.7545	7.3805	7.261	7.9215	7.698
Crude Energy (MJ/100 g)	1.653	1.676	1.651	1.642	1.644	1.616
Protein /Energy (MJ/100 g)	12.898	17.962	24.414	27.492	30.565	35.819

 

a 100% Casein complex, Scitec Nutrition (73% crude protein, 2.3% crude fat, 14.7Kj.g-1 gross energy, Miami, USA)

b Ghana farming network Ltd (64% crude protein, 9.6% crude fat, 18.7 Kj.g-1 gross energy, Accra, Ghana)

c Saccharomyces cervisiae, S.I. Lesaffre (56% crude protein, 0% crude fat, 16.72 Kj.g-1 gross energy, France)

d Nestlé HealthCar Nutrition GmbH, D-67574 Osthofen (0.2% crude protein, 0% crude lipid, 16.18 Kj.g-1 gross energy)

e SIGMA-ALDRICH (0% cruds protein, 0% crude lipid, 15.94 Kj.g-1 gross energy, France)

f Setalg, France (0% crude protein, 100.0% crude lipid, 37.00 Kj.g-1 gross energy, France)

g Songhaï center (0% crude protein, 99.9% crude lipid, 37.00 Kj.g-1 gross energy, Benin)

h Vitamin premix contains (g 100 g-1 of premix) : ascorbc acid, 50.0 ; D-calcium pantothenate, 5.0 ; choline chloride, 100.0 ; inositol, 5.0 ; menadione, 2.0 ; niacin, 5.0 ; pyridoxine HCL, 1.0 ; riboflavin, 3.0 ; thiamin HCL, 0.5 ; DL-alpha-tocopherol acetate (250 IU g-1), 8.0 ; vitamin A acetate (20,000 IU g-1), 5.0 ; vitamin micro-mix, 10.0 ; cellulose, 805.5 ; Vitamin micro-mix contains (g.kg-1 of micro-mix) : biotin, 0.5 ; cholecalciferol (1µg = 40 IU), 0.02 ; folic acid, 1.8 ; vitamin B12, 0.02 ; cellulose, 97.66.

i Mineral premix contains (g kg-1 of premix) : calcium phosphate (monobasic) monohydrate, 136.0 ; calcium lactate pentahydrate, 348.49 ; ferrous sulfate heptahydrate, 5.0 ; magnesium sulfate heptahydrate, 132.0 ; potassium phosphate (dibasic), 240.0 ; sodium phosphate (monobasic) monohydrate, 88.0 ; sodium chloride, 45.0 ; aluminium chloride hexahydrate, 0.15 ; potassium iodide, 0.15 ; cupric sulfate pentahydrate, 0.50 ; manganese sulfate monohydrate, 0.70 ; cobalt chloride hexahydrate, 1.0 ; zinc sulfate heptahydrate, 3.0 ; sodium selenite, 0.011.

 

 

Nowadays, H. occipitalis is one of reared frogs in Africa countries such as Rwanda, Ivory Coast, and Benin etc. This species is also reared in Philippines and Malaysia due to its quick growth and tenderness of its meat (Tokur et al., 2008). Consequently, it is important to assess nutritional needs of H. occipitalis in order to master feed formulation for its rearing. Domestication and intensive rearing of H. occipitalis must start from providing information on raniculture and frog species diversification all over the world. In intensive larval rearing, feed protein rate is one of factors which influence the survival rate, the well-being and the growth (NRC, 1993), feed ration (Godome et al., 2018a) and stocking density (Schram et al., 2006; Godome et al., 2018b). It is therefore necessary to determine the appropriate feed protein rate on which depend nutrients and energy availability for speeded growth of the animal. Economically, knowing protein needs is essential for shortening larval rearing period because the aim of frog rearing is not getting big tadpoles but the production (Carmona-Osalde et al., 1997). Protein is the most expensive element in aquatic animals feed (Moreira et al., 2008). So, the use of agricultural by-product and vegetal protein genuine is economically and environmentally preferable. According to Oishi et al. (2010), the use of optimal protein for aquatic species prevents high environmental pollution especially in intensive rearing. It is so important to determine protein quantity necessary for rapid growth of H. occipitalis in order to avoid pollution of the rearing medium. It is also fundamental for formulation of feed diet suitable for any development stage of any species reared in captivity. Growth rate has often been used as physiological criterion for nutritional evaluation in many species. Numerous studies showed the importance of protein needs on survival and growth of R. catesbeiana (Chun-xiao et al., 2016; Olvera-Novoa et al., 2007; Prapee et al., 1997; Carmona-Osulde et al., 1997), R.esculanta (Tokur et al., 2008), but published studies explaining nutritional needs of H. occipitalis are almost inexistent nowadays. The current study aims to determine the optimal ration of H. occipitalis tadpoles.

 

MATERIAL AND METHODS

 

Formulation and Preparation of Feed Diets

Six isoenergetic experimental diets P1, P2, P3, P4, P5, and P6 were formulated with semi-purified ingredients. Protein rates in these diets are 20, 30, 40, 45, 50 and 60 g / 100 g respectively (Table 1). According to protein needs of tadpoles Rana catesbeiana belonging in the same order as H. occipitalis (Chun-Xiao et al., 2016; Prapee et al., 1997; Olvera-Novoa et al., 2007; Carmona-Osulde et al., 1997). The dry ingredients were crushed in a grinder, weighted, mixed and homogenized manually in a plastic bowl. Then, oil and water were added to obtain a soft paste that was minced (Moulinex HV8) in 0.2 – 0.8 mm diameter spaghettis. After drying in lyophilisator (Eyela Dry Chamber DRC-1N), spaghettis were manually fragmented in small grains and stored at 18 °C.

 

Origin of Experimental Tadpoles, Rearing Conditions and Feeding

H. occipitalis tadpoles (initial weight = 0.04 ± 0.02 g) used in the current study were obtained from artificial reproduction on the experimentation station of the Laboratory of Research on Wetlands of the University of Abomey-Calavi. Therefore, 72 hours after eggs hatching, tadpoles were transferred in circular ponds. They were fed, for one week, on live preys (zooplankton and nauplii of Artemia salina) which were then progressively substituted by commercial feed (Coppens). Then, in order to accustom Tadpoles to experimental diets, they were fed on a mixture of these diets. Tadpoles were reared like that till reaching about 0.38 g. The experiment was carried out for 24 days in 18 circular ponds containing 30 L water each one. These acclimated tadpoles (0.380 ± 0.004 g) were then distributed in 18 circular ponds containing 30 L water each one with at a stocking density of 55 tadpoles/pond. During the experiment, tadpoles were manually and hourly from 8 am to 6 pm everyday at a ration of 6% tadpoles biomass.

 

Water quality were monitored by measurement of temperature, dissolved oxygen and pH using. Portable multimeter (Calypso ORCHIDIS SN-ODEOA 2138) at 7:30 am and 4:30 pm. Therefore, Mean values of water temperature, pH and dissolved oxygen in the experimental media were 28.33 ± 0.09 °C, 3.98 ± 0.02 mg.L-1n 7.01 ± 0.01 and respectively. Water were renewed twice per day at 7 am and 17 pm before and after feeding with flow 0.5 L per minute.

 

Control fishing was done every three days following by emptying and cleaning of ponds. Tadpoles were then counted and biomass was evaluated in each pond in order to adjust feed ration. After 24 days rearing, tadpoles were collected from ponds and weighted individually. Data collected enabled calculation of some parameters such as weight gain (WG), specific growth rate (SGR), survival rate (SR), feed conversion rate (FCR), ingested feed (IF), ingested protein (IP) and protein efficiency ratio (PER) were calculated through the following formulas:

 

WG (%) = 100 × [final mean weight (g) – initial mean weight (g) / initial mean weight]

SGR (%/D) = 100 x [ln (final mean weight) – ln (initial mean weight)]/experiment duration

SR (%) = 100 x (final number / initial number)

FCR = ingested feed quantity (g) / weight gain (g)

IF = (supplied dry feed – remained dry feed) / tadpoles number

IP (%) = [Protein rate of diet × ingested feed] / 100

PER = weight gain (g) / ingested protein quantity.

 

At the end of experiment, fifteen bulbous crowfoots were randomly sampled from each pond and sacrificed for chemical analysis. Analysis of dry matter, crude proteins, crude lipids and ash were assessed in experimental feed and bulbous crowfoots carcass according to the method of AOAC (2012).

 

Data Analysis

Data collected enabled zootechnical parameters calculation. Mean and range were calculated for each parameter. Mean values for each replication were considered as an observation.

 

The R (version 3.5.0) software was used for comparisons with 5% probability threshold. One way analysis of variance (ANOVA 1) was carried out to compare zootechnical performances of the different treatments. The Student-Newman-Keuls test (SNK test) was carried used pair comparisons of means in order to determine the optimal protein rate and survival rate of H. occipitalis tadpoles. The model of Brett and Grove (1979) was applied to the polynomial regression II between the specific growth rate and protein rate to determine optimal and maximal protein requirements of H. occipitalis tadpoles.

 

RESULTS

 

Water Quality

During the experiment, pH and dissolved oxygen of water have not been significantly influenced by the treatments (P > 0.05). In return, temperature values varied significantly among treatments (P < 0.05) (Table 2).

 

Table 2: Mean values ± standard deviation of pH, temperature and dissolved oxygen (DO) of the differents treatments during the experiment.

 

Parameters	P1 (20%)	P2 (30%)	P3 (40%)	P4 (45%)	P5 (50%)	P6 (60)
pH	7.375±0.043a	7.258±0.080a	7.258±0.080a	7.308±0.014a	7.150±0.156a	7.158±0.072a
T°C	29.783±0.005a	29.653±0.105a	29.436±0.030b	29.343±0.053b	29.165±0.133c	28.866±0.034d
DO (mg/L)	8.366±0.038a	8.374±0.596a	7.791±0.920a	7.766±0.500a	6.516±1.432a	7.400±1.047a

 

abcd mean values on same line, following by different letters are significantly different (P < 0.05).

 

Table 3: Growth and nutrients utilization parameters in Hoplobatrachus occipitalis tadpoles fed different protein rate for 24 days.

 

Parameters	P1	P2	P3	P4	P5	P6
	20%	30%	40%	45%	50%	60%
Bi	20.93 ± 0.234a	20.436 ± 1.250a	19.996±0.883a	19.783 ± 0.872a	21.203 ± 0.322a	21.130±0.364a
Bf	31.966±1.799c	33.660±0.192c	39.486±0.927b	39.246±1.221b	38.616±1.211b	42.626±1.740a
DWG (g/D)	0.195±0.015d	0.291±0.035c	0.411±0.016ab	0.422±0.026ab	0.393±0.016b	0.454±0.017a
SGR (%/D)	1.729±0.124c	2.415±0.329b	3.152±0.025a	3.238±0.210a	2.930±0.117a	3.253±0.087a
IF	0.249±0.012d	0.306±0.011c	0.391±0.005b	0.468±0.041a	0.478±0.026a	0.507±0.007a
IP	2.425±0.097e	4.593±0.249d	7.993±0.038c	10.404±0.863b	11.248±0.776b	14.932±0.433a
FCR	1.117±0.163a	1.169±0.176a	1.025±0.016a	1.187±0.033a	1.295±0.102a	1.161±0.063a
PER	4.535±0.612a	2.893±0.431b	2.438±0.039b	1.872±0.053c	1.550±0.119c	1.438±0.080c
SR (%)	88.484±1.049b	90.909±1.818ab	92.727±1.818a	89.696±1.049ab	85.454±1.818c	89.090±1.818ab

 

abcde values on same line and non affected by same letter are significantly different (P < 0.05).

Table 4: composition of Hoplobatrachus occipitalis tadpoles fed on six different protein rates for 24 days.

 

Parameters	Control	P1 (20%)	P2 (30%)	P3 (40%)	P4 (45%)	P5 (50%)	P6 (60%)
Dry matter	78,634± 3,126b	89,857± 0,011a	89,150± 0,048a	89,407± 0,009a	90,551± 0,020a	90,843± 0,037a	89,825± 0,047a
Ash	11,791± 0,010g	14,841± 0,008d	15,574± 0,005b	13,686± 0,014f	15,410± 0,030c	14,749± 0,011e	16,054± 0,014a
Crude lipid	8,349± 0,901e	10,734± 0,092bc	11,414± 0,336b	10,55± 0,384c	9,558± 0,284d	12,396± 0,165a	9,206± 0,002d
Crude protein	36,783± 0,592c	43,491± 0,001b	43,217± 0,003b	35,606± 0,003d	47,327± 0,005a	44,053± 0,004b	36,799± 0,004c

 

abcdefg values on same line and not affected by same letter are significantly different (P< 0.05).

 

Survival And Growth Of Tadpoles

Growth parameters, feed utilization and survival rate of H. occipitalis tadpoles are mentioned in Table 3. For the current experiment, survival rate was between 85.454 ± 1.818 and 92.727 ± 1.818%. The highest survival rate was obtained with tadpoles fed on diet P3 (40% protein). However, apart from tadpoles fed on 50% protein diet (P5) presenting the lowest value, any significant difference was not observed (P ˂ 0.05) among treatments. Specific growth rate, final mean weight and protein efficiency ratio were significantly influenced (P ˃ 0.05) by the different diets protein rate. Mean biomasses of tadpoles fed on 40, 45, 50 and 60% protein diets doubled almost after the 24 days. The highest final biomass was obtained with the 60% protein diet (P6) (Figure 1). The best specific growth rate (3.253 ± 0.087 %/D) was also noticed with the diet P6. However, there was no significant difference (P > 0.05) among treatments P3 to P6. The highest mean values of feed conversion rate was obtained in P4 (45% protein) treatment but any significant difference was not observed among feed conversion rates. The same report was done for weight but with significant difference (P > 0.05) among treatments. Protein efficiency ratios were significantly influenced (P ˂ 0.05) by treatments. The highest mean value was obtained in the 20% protein treatment (P1).

 

Approximate Composition of Tadpoles’ Carcass

The composition of H. occipitalis tadpoles carcass at the end of experiment is mentioned in Table 4. Proteins, lipids, ash and dry matters were significantly affected (P ˂ 0.05) by the protein rates. The carcass of tadpoles fed on diet P4 (45%) presented the highest protein rate though the lowest was recorded in the carcass of tadpoles fed on diet P3 (40%). Lipid value of the carcass of tadpoles fed on diet P5 (50%) was the significantly highest. Generally, dry matter, lipid, protein and ash in the initial sample were lower than those after tadpoles feeding.

 

Estimation of Protein Needs

Mean values of specific growth rate and feed conversion rate showed the best feed were P6 (60%) followed successively by P4 (45%), P3 (40%), and P5 (50%). According to Brett and Grove (1979) method expressing relationship between feed protein rates and specific growth rate, optimal and maximal protein needs for a good growth of H. occipitalis tadpoles are respectively 33 % and 51 % (Figure 2).

 

 

 

DISCUSSION

 

Temperature is the major factor influencing frog growth because it interferes directly with their metabolism (Petersen and Gleeson, 2011). Besides, temperatures recorded during the current study were within the range 15 to 35°C recommended for good growth of tadpoles (Petersen and Gleeson, 2011). Values of pH (7.150 ± 0.156 – 7.375 ± 0.043) and dissolved oxygen (6.516 ± 1.432 – 8.374 ± 0.596 mg/l) obtained during the experiment are in accordance with those recorded by certain authors (Flores-Nava and Vera-Muñoz, 1999; Borges et al., 2014) in Lithobates catesbeianus tadpoles.

 

After the 24 days feeding experiment, survival rates recorded for treatments varied from 85.454 ± 1.818 to 92.727 ± 1.818 %. These survival rates are higher than those (83.33 ± 0.10 - 90.48 ± 0.11 %) recorded for Calyptocephalella gayi by Pedro et al. (2014) but similar to those obtained by Rungkan, 2015 (75.00 ± 2.36 - 92.22 ± 1.92 %) with R. rugulosa, those of Zhang et al. (2016) with R. catesbeiana and Godome et al. (2018a) H. occipitalis. During the experiment, the highest mortality was observed in tadpoles fed on diet P1 (20%) and P2 (30%). These mortalities seem due to the protein rates of these diets which are lower than the least protein rate (40%) recommended in frogs feed (Carmona-Osalde et al., 1997). Indeed, in predatory species, adaptation to artificial feed affects their survival rate (Durville et al., 2003).

 

SGR recorded in the current experiment (1.729 ± 0.124%/D and 3.253 ± 0.087 %/D) are different from those recorded for Calyptocephalella gayi (1.02 ± 0.08 %/D, Pedro et al., 2014), tadpoles of Rana rugulosa (2.79 ± 0.19 %/D, Rungkan, 2015) and Rana catesbeiana (1.86 ± 0.02 %/D, Zhang et al., 2016). According to Flores-Nava et al. (1994), the height of tadpoles fed on low protein level varies more than those fed on high protein level. The lowest SGR were obtained for tadpoles fed on P1 diet. This low SGR recorded by P1 could be tied to the low protein rate (20% protein) of this diet since feed protein level affects tadpoles’ growth rate. Indeed, metamorphosis can be achieved unless tadpoles satisfy their protein requirements (Carmona-Osalde et al., 1997). A study carried out by Martinez et al. (1993) with Rana perezi tadpoles showed their development depends on feed nutrients. Insufficient feeding also elongates larval period.

 

It is important to notice that inadequate feed protein rate leads to growth reduction and weight loss. Therefore the lowest growth obtained by diets 20% and 30% may be due to the fact major part of protein was used for maintenance instead of growth.

 

Feed conversion rate (FCR) and protein efficiency ratio (PER) can be qualified as excellent feeding and protein utilization indicators in aquaculture. FCR recorded during the current study are lower than those recorded for Rana catesbeiana (Olvera-Novoa et al., 2007; Flores-Nava and Vera-Muñoz, 1999; Gogome et al., 2018a). These low FCR obtained may be due not only to the quality of feed diets but also to experimental conditions such as difference among species, stocking density, protein quality and environmental conditions (Jauncey and Ross, 1982; Al-Hafedh, 1999). In this study PER decreases with the protein levels. These results are close to those reported by Olvera-Novoa et al. (2007) but upper than trends recorded for Rana catesbeiana tadpoles (Carmona-Osalde et al., 1997).

 

Crude protein rates recorded in tadepoles’ carcass reared in this study are highly lower than those reported by Thirumalai et al. (1977) in frog (70.8%) and Tokur et al. (2008) in R. esculanta tadpoles (58.1%). In contrary, they are largely upper than those recorded in R. catesbeiana tadpoles (17.39 % and 14.61 %) by Zhang et al. (2016) and Olvera-Novoa et al. (2007) respectively. That could be explained by difference among species the rearing conditions and the feed composition. Lipid rate (7.1%) noticed in H. occipitalis tadepoles’carcass is higher than that reported by Thirumalai et al. (1977) in frog carcass which ash rate (22.1%) is though the current. Besides, Rao and Kamasastri (1963) observed highest fat rate (8.4%) lower than our results based on dry weight. This difference can be explained by the fact mentioned studies were carried out on the same species.

 

According to model of Brett and Grove (1979) used for evaluation of feed protein rate in relation to SGR, the optimal and the maximal protein rates for good growth of H. occipitalis tadpoles are 33% and 51% respectively. This feed protein rate optimal can be useful for optimization of larval rearing period. Prior nutritional studies on Rana catesbeiana tadpoles indicated the best performance was obtained in diet with about 40% protein rate (Carmona-Osalde et al., 1997). Our protein rates recorded are nearby those (20 to 70%) recorded by Culley et al. (1978) or 20 to 50% (Fontanello et al. 1982), either 33 to 40% (Mazzoni and Camevia, 1988). Roughly, protein rate obtained in the current study is a little close (40%) to those reported by Fontanello et al. (1985).

 

CONCLUSION

 

The results of this study indicate that the highest daily weight gain (DWG) and specific growth rate (SGR) were obtained by diet containing 60% of protein. In relation of SGR, protein needs of H. occipitalis tadpoles varied from 33 to 51 % representing the optimum and maximum respectively. Higher feed protein rate leads not only to an increase in protein efficiency ratio but also to excess lipid accumulation in H. occipitalis tadpoles’ carcass.

 

ACKNOWLEDGMENTS

 

Authors give a thank to the Worldwide Bank through the African Center of Excellence on Climate Change, Biodiversity and Sustainable Agriculture (ACE-CCBSA) for its financial support granted to Théophile GODOME. We also thank Landry ALAPINI, Mahunan Tobias C. AZON and James G. ZANNOU for their contribution.

 

Conflict of Interest

 

There is no conflict of interest.

 

AUTHORS’ CONTRIBUTION

 

All authors contributed equally in this study and writing of the manuscript.

 

REFERENCES

 

• Al-Hafedh YS (1999). Effects of dietary protein on growth and body composition of Nile tilapia, Oreochromis niliticus L. Aquacult. Res. 30: 385-393. https://doi.org/10.1046/j.1365-2109.1999.00343.x.

• AOAC (Association of Official Analytical Chemists) (2012). Official methods of analysis of the Association of Official Analytical Chemists, 19th edition. AOAC, Arlington, Virgina, USA.

• Borges FF, Stéfani MV, Amaral LA (2014). Quality of the effluents of bullfrog tadpole ponds, Bol. Inst. Pesca, São Paulo. 40(3): 409 – 417.

• Brett JR, Groves TDD (1979). Fish physiology. Physiolog. Energet. 3: 280-282.

• Cairns AM, Bock JW, Bock FG (1967). Leopard frogs raise in partially controlled conditions. Nature. 213: 191-193. https://doi.org/10.1038/213191a0

• Carmona-Osalde C, Olvera-Novoa MA, Rodrìguez-Serna M, Flores-Nava A (1997). Estimation of the protein requirement for bullfrog (Rana catesbeiana) tadpoles, and its effect on metamorphosis ratio. Aquuculture. 141: 223-231 https://doi.org/10.1016/0044-8486(95)01232-X.

• Chardonnet Ph, des Clers B, Fischer J, Gerhold R, Jori F, Lamarque F (2002). The value of wildlife. Revue Scientifique Et Technique De l’Office International Des Epizooties. 21(1): 15–51. https://doi.org/10.20506/rst.21.1.1323

• Chun-Xiao Z, Kang-kang H, Ling W, Kai S, Kang-le L, Lu Z, Peng L (2016). Optimal dietary carbohydrate to lipid ratio for bullfrog Rana (Lithobates) catesbeiana. Aquacult. Res. 47: 3332–3340 https://doi.org/10.1111/are.13109

• Crumlish M, Inglis V (1999). Improved disease resistance in Rana rugulosa (Daudin) after b-glucan administration. Aquacult. Res. 30: 431–435. https://doi.org/10.1046/j.1365-2109.1999.00345.x

• Culley JrDD, Horseman ND, Amborski RL, Meyers SP (1978). Current status of amphibian culture with special emphasis on nutrition, diseases and reproduction of the bullfrog, Rana catesbeiana. In: J.W. Avault (Editor), Proceedings of the Ninth Annual Meeting of the World Mariculture Society, Louisiana St. Univ. Baton Rouge, LA. Pp. 653-669.

• Durville P, Bose P, Galzin R, Conand C (2003). Aptitude à l’élevage des post-larves de poissons coralliens. Res. Marines Commercialis. 11 : 19 – 30.

• FAO (Food and Agricultural Organisation) (2006). Food and agricultural organisation, cultured aquatic species information programme – Rana catesbeiana. http://www.fao.org/figis/servlet/static?xml=/Rana_catesbeiana.xml-@@ dom=culturespecies

• Flores-Nava A, Olvera-Novoa MA, Gasca-Leyva E (1994). A comparison of the effects of three water-circulation regimes on the aquaculture of bullfrog (Rana catesbeiana Shaw) tadpoles. Aquaculture. 128: 105-114. https://doi.org/10.1016/0044-8486(94)90106-6

• Flores-Nava A, Vera-Muñoz P (1999). Growth, metamorphosis and feeding behaviour of Rana catesbeiana Shaw 1802 tadpoles at different rearing densities, Aquacult. Res. 30: 341-347. https://doi.org/10.1046/j.1365-2109.1999.00335.x

• Fontanello D, Arruda-Soares H, Mandelli JrJ, Reis JM (1982) Desenvolvimento ponderal de girinos de RI-tour0 Rana catesbeiana (Shaw, 1802) & ados corn raqao de diferentes niveis proteicos. Boletin Institute de Pesca, SZo Paulo. 9: 125- 129.

• Fontanello D, Arruda SH, Mandelli JrJ, Justo CL, Penteado LA, Campos BES (1985). lnfluencia da proteina de origem animal e vegetal no desenvolvimento ponderal de girinos de Rana catesbeiana, Shaw, 1802. criados em ranario experimental. Boletin lnstituto de Pesca, SZo Paula. 12 (2): 43-47.

• Godome T, Tossavi CE, Ouattara IN, Fiogbe ED (2018a). Determination of the optimal feed ration for best growth of Hoplobatrachus occipitalis (Günther, 1858) tadpoles reared in controlled medium. Int. J. Fisheries Aquat. Stud. 6(2): 376-380.

• Godome T, Tossavi E, Djissou A, Zounon Y, Ouattara NI, Fiogbe ED (2018b). Effect of stocking density on the survival and growth of Hoplobatrachus occipitalis (Günther, 1858) (Amphibia: Dicroglossidae) of tadpoles reared in Ponds from Benin, Int. J. Aquacult. 8(18): 137-144. https://doi.org/10.5376/ija.2018.08.0018

• Hirschfeld JW, Richards CM, Nace GW (1970). Growth of larval and juvénile Rana pipiens of four laboratory diets. Amer. Zool. 10: 315.

• Jauncey K, Ross B (1982). A guide to Tilapia Feeds and Feeding. Institute for Aquaculture, University of Stirling, UK.

• Martinez IP, Herraez MP, Alvarez R (1993). Optimal level of dietary protein for Rana perezi Seoane larvae, Aquaculture and Fisheries Management. 24: 271-278. https://doi.org/10.1111/j.1365-2109.1993.tb00550.x

• Miles J, Williams J, Hailey A (2003). Frog farming: Investigation of biological and mechanical agents to increase the consumption of pelleted food by adult Rana temporaria. Appl. Herpetol. 1 (3–4), 271–286.

• Moreira IS, Peres H, Couto A, Enes P, Oliva-Teles A (2008). Temperature and dietary carbohydrate level effects on performance and metabolic utilisation of diets in European sea bass (Dicentrarchus labrax) juveniles. Aquaculture. 274: 153–160. https://doi.org/10.1016/j.aquaculture.2007.11.016

• Mazzoni R, Camevia D (1988). Estado actual de la ranicultura en Umguay. Mem. VI Encontro National de Ranicultores, Rio de Janeiro, Brasil. Pp. 117-187.

• Negroni G (2003). Frog marketing with particular reference to EU countries. World Aquaculture 2003, meeting abstract.

• NOAA (National Oceanic and Atmospheric Administration) (2005). Fisheries statistics division imports and exports of fisheries products annual summery. Current Fisheries Istatistics No. 2005-2. <http://www.st.nmfs.gov/st1/index.html>.

• NRC (National Research Council) (1993). Nutrient Requirements of Fish. National Academy Press, Washington, DC. 114 pp.

• Oishi CA, Nwanna LC, Pereira-Filho M (2010). Optimum dietary protein requirement for Amazonian Tambaqui, Colossoma macropomum (Cuvier, 1818), fed fish meal free diets. Acta Amazonica. 40 : 757-762. https://doi.org/10.1590/S0044-59672010000400017

• Olvera-Novoa MA, Ontiveros-Escutia MV, Flores-Nava A (2007). Optimum protein level for growth in juvenile bullfrog (Rana catesbeiana Shaw, 1802). Aquaculture. 266: 191–199. https://doi.org/10.1016/j.aquaculture.2007.02.013

• Pedro HT, Ricardo S, Maria TV (2014). Formulated diets for giant Chilean frog Calyptocephalella gayi tadpoles. Ciencia e Investigación Agraria. 41(1):13-20.

• Petersen AM, Gleeson TT (2011). Acclimation temperature affects the metabolic response of amphibians skeletal muscle to insulin. Comparat. Biochem. Phisiol. 160(3): 72-80. https://doi.org/10.1016/j.cbpa.2011.05.005

• Prapee S, Pornchai L, Maleeya K, Suchart UE (1997). Effects of diets with various protein concentrations on growth, survival and metamorphosis of Rana tigerina and Rana Catesbeiana. J.Sci.Soc. Thailand. 23: 209-224. https://doi.org/10.2306/scienceasia1513-1874.1997.23.209

• Priddy JM, Culley DDJr (1971). The frog culture industry, past and present. Proceedings Annual Conference Southeastern Association of Came and Fish Commissioners. 25: 597-601.

• Rao DR, Kamasastri PV (1963).Utilization of waste materials in frog leg processing industry. Indian J. Fisher. 10 (1), 4–7.

• Rungkan K (2015). Growth Performance and Feed Utilization of Common Lowland Frog (Rana rugulosa Wiegmann) Fed with Supplementation by Bromelain Extracted from Pineapple Feed. Int. J. Environmen. Rural Develop. 1-6.

• Schram E, Van der Heul JW, Kamstra A, Verdergem MCJ (2006). Stocking density dependent growth of dover (Solea solea). Aquaculture. 252: 239 – 247. https://doi.org/10.1016/j.aquaculture.2005.07.011

• Somsueb P, Boonyaratpalin M (2001). Optimum protein and energy levels for the Thai native frog, Rana rugulosa Weigmann. Aquacult. Res. 32 (1), 33–38. https://doi.org/10.1046/j.1355-557x.2001.00033.x

• Thirumalai S, Vedanayagam K, Rajagopalan G, Venkatakrishnan R(1977). Preliminary investigation on frog meal as an animal protein source for poultry. Cheiron. 6: 2–5.

• Tokur B, Gürbüz D, Özyurt G (2008). Nutricional composition of frog (Rana esculanta) waste meal. Bioresource Technol. 99(6): 1332-1338. https://doi.org/10.1016/j.biortech.2007.02.032

• Zhang C-x, Huang K-k, Wang L, Song K, Lu K-l, Zhang L, Peng LiP (2016). Optimal dietary carbohydrate to lipid ratio for bullfrog Rana (Lithobates) catesbeiana. Aquacult. Res. 47: 332–334. https://doi.org/10.1111/are.13109