Usman Ijaz1, Muhammad Sajid Tahir2, Khalid Abdul Majeed3*, Shahid Iqbal4, Iffat Huma5, S. Firyal6, Ijaz Ahmed7, Shahid Chohan8 and Aamir Riaz Khan9
...p;Panthera pardus subspecies found in Pakistan by exploring the partial DNA sequences of mitochondrial Cytochrome c Oxidase subunit I (COI) gene. Scat samples of 15 different known leopards were collected from Nathiagali, Khyber Pakhtunkhwa, Pakistan and Azad Jammu and Kashmir, Pakistan. After amplification with specific oligos, the amplicons of partial region of COI were subjected to sequencing, and then observed for single nucleotide polymorphisms (SN...
Paul I. Ankeli, Mashood A. Raji, Haruna M. Kazeem, Moses O. Odugbo, Nendir J. Umaru, Idowu O. Fagbamila, Livinus T. Ikpa, Obinna O. Nwankiti, Issa A. Muraina, Pam D. Luka and Nicholas D. Nwankpa
...tify Mycoplasma mycoides subspecies mycoides (Mmm) from the ear canal of apparently healthy cattle in Plateau State, Nigeria. One hundred and sixty six ear swab samples (n=166) were cultured from which eight (8) Mycoplasma species were isolated and characterized using conventional biochemical tests and polymerase chain reaction (PCR), respectively. Six (6) were observed to ferment glucose, reduce tetrazolium chloride and hydrolysed casein. They had no phosphat...
Abdullah Mart1* and Orhan Erman2
...wenty nine species and 2 subspecies belonging to 10 genera of water scavenger beetles (Coleoptera: Hydrophilidae) collected from Bingöl province between May 2003-October 2004 have been described....
Tianzhu Chao, Huiqiang Cai, Yuxun Zhou, Kai Li* and Junhua Xiao
...ustify;">The patterns of subspecies distribution, diversity and differentiation of the house mouse Mus musculus provide insights into past dispersal events in natural populations of this species. We investigated the molecular phylogenetics of Chinese house mice based on 466 DNA sequences from mitochondrial D-loop fragments. Our analyses revealed that Mus musculus musculus and M. m. castaneus are older colonized sub...
Song Li1,* and Zuojian Feng2
... we describe them as new subspecies P. a. muzongensis subsp. nov. Discussion on the relationships between the differentiation of P. albiventer and its environmental evolution in southwestern China are also provided.
...
Yanxiao Meng1, Guihua Wang1, Dongmei Xiong1,*, Haixia Liu1, Xiaolin Liu1, Lixin Wang1 and Jianlu Zhang2
...ate on the validation of subspecies B. lenok tsinlingensis Li for a long time. Some ichthyologists thought that there should be two species (B. lenok and B. tumensis) in Amur River and a subspecies (B. lenok tsinlingensis) in the Qinling Mountains, while others believed no division of the subspecies. Thus, 169 specimens of Brachymystax spp. were co...
Wei Meng1,3, Tianyan Yang2,*, Yunguo Liu1, Mahmut Halik1 and Tianxiang Gao2
... be a cryptic species or subspecies of G. dybowskii. The phylogenetic analyses from 12 concatenated H-strand-encoding protein genes were conducted by Neighbor-Joining method to reveal the evolutionary relationships within subfamily Schizothoracinae. Three different grades of schizothoracine fishes were well recognized from each other in branching diagram. The primitive group and the specialized group + the highly specialized group constituted a sister r...
Fehmeeda Fatima, Asif Nadeem* and Maryam Javed
...thi) is a vulnerable subspecies of wild goat (Capra aegagrus), which faced serious population threats. In the present study, HSP70.1 gene has been partially sequenced (1344 bp) to study genetic diversity and evolutionary characteristics of Sindh ibex. In this research, samples of Sindh ibex were collected from Kirthar National Park, Sindh, Pakistan, the gene of interest was amplified, PCR products were sequenced and data was analyzed. The results of...
Aiguo Zhou1,3,4, Shaolin Xie1,4, Zhenlu Wang1, Lanfen Fan1, Yanfeng Chen2, QiaoYe1, Fang Zeng1 and Jixing Zou1,*
...he same species, but not subspecies at the molecular level. Moreover, take Lateolabrax maculates and Epinephelus coioides as outgroups, molecular phylogenetic tree showed that all the haploids gather together as a branch and crossing each other. These indicated that the white type Channa argus should be regard as an albino of biocolor type.
...
Zhiwen Zou, Jianfei Xi, Fen Chen, Rui Xu, Tianrong Xin and Bin Xia*
...blyseiinae genus and the subspecies was studied. The results showed that four species of Kampimodromus and two species of Euseius clustered into two different branches, but the branch of 8 species of Amblyseius and Neoseiulus was chaotic. It indicated that the species of Neoseiulus and Amblyseius should be assigned carefully. The results could not only reveal its phylogenetic relationship and provide molecular evidence...
Rais Ahmed1,2,*, Muhammad Khalid Mansoor1,2, Iftikhar Hussain1, Muhammad Saqib3, Muhammad Hammad Hussain4, Amjad Islam Aqib5, Haleema Sadia6, Javed Muhammad7, Asma Irshad8, Kashif Prince5, Muhammad Zain Saleem9 and Abdul Whab Manzoor10
Muhammad Rizwan Ashraf1, Asif Nadeem1,2, Eric Nelson Smith3, Maryam Javed1, Utpal Smart3, Tahir Yaqub4, Abu Saeed Hashmi1 and Panupong Thammachoti3
...quired to raise both the subspecies to separate species.
... 
Muhammad Medrar Hussain*, Asad Jan* and Sayyar Khan Kazi
...xpressed in Oryza sativa subspecies indica variety Swat-1. Through Agrobacterium mediated transformation, OsTZF8 gene (pBIG-Ubi-OsTZF8) was successfully transferred to rice calli. From these rice calli transgenic plants were regenerated and established in greenhouse. For the evaluation of OsTZF8 gene role in drought stress tolerance, two weeks old rice seedling were subjected to drought stress. OsTZF8-OX transgenic indica line A and B displayed 69% and 64% sur...
Jam Nazeer Ahmad1,*, Samina J.N. Ahmad1,2,*, Mubashir Ahmad Malik1, Abid Ali1, Muhammad Ali3, Ejaz Ahmad1, Muhammad Tahir2 and Muhammad Ashraf2
...ee with that of specific subspecies S. g. gregaria which furtherdeviate genetically not only from closely related subspecies (S. g. flaviventris) but also other species reported from various countries. Migratory pattern of S. g. gregaria from Africa to Asia and neighboring countries with their potential routes also suggested developing sustainable policy to counter internal as well as external threat. Th...
Kiran Shahjeer1*, Gauhar Rehman1, Khurshaid Khan1 and Toheed Iqbal2
... simplex, L. opaca and a subspecies of L. opaca i.e. L. o. pulchella are reported as first distributional records from Khyber Pakhtunkhwa. Keys to the subfamilies of Family Formicidae and worker caste species of genus Lepisiota collected from Khyber Pakhtunkhwa along with its spatial distributional map for all species and a sub-species is provided. All the specimen are deposited at Insect Museum, Department of Zoology, Abdul Wali Khan University Mardan, Pakist...
Ghulam Raza1*, Maqsood Anawar2, Muhammad Akbar1, Muhammad Ali1, Alamdar Hussain1, Azhar Hussain3 and Tanveer Hussain4
... ibex sibirica) is a subspecies of Capra ibex, and is evenly distributed throughout the upper comparatively dry mountains of Gilgit-Baltistan, Pakistan. It is categorized as a Least Concern species globally as well as in Pakistan, however its presence in the Karakorum mountain plays a significant role in the economy of the region as dozens of trophy hunting of this specie takes place in the region every year and generates handsome money to th...
Hadeer M. Mossa, Ausama A. Yousif, Emad A. Aboelsoud, Mahmoud El Gamal, Ahmed El-
Sanousi
...t was then classified to subspecies and strain level in a
quantitative manner in approximately 48min and 23 second.
Conclusion: This method allows screening all the virus harvest content in non-selective, non- targeted
manner. The reasons to choose this technology in qualification of the TC propagated LSDV as a onestep
quality control for in process LSDV indicating all what in the sample as well as an indication ...
Muhammad Tariq Khan1, Muhammad Ather Rafi2, Riffat Sultana3*, Anjum Munir1 and Sajjad Ahmad4
...d list of 10 species and subspecies of subfamily Eumeninaein in Sindh province under 5 genera i.e. Delta, Knemodynerus, Antepipona, Allorhynchium, Antodynerus. Besides this, their bio-geographical affiliations have also been discussed. Of these species and subspecies 6 are part of the Oriental fauna, one is part of the Afrotropical and Palearctic Region and three belong to Oriental, Afrotropical and Palearctic Regions. This ...
Cheng Zhao1,2, Qin Shi1*, Gen Yang3, Youyuan Tan2, Songwen Tan2, Jiahong Li2 and Marwan M.A. Rashed2
...huanicus is a threatened subspecies of sika deer. Winter is the critical season affecting the survival of Sichuan sika deer. To get strategic information about the relationship between Sichuan sika deer and the environment in the winter, we conducted our study on habitat use of Sichuan sika deer in the Tiebu Nature Reserve from January to February in 2019 and 2020. The results showed that Sichuan sika deer preferred shrub vegetation and southern slope. Compare...
Tariq Mahmood1*, Mamoona Wali Muhammad2, Sami Ullah1, Bilal Ahmad3, Zarmina Aslam4, Naveed Ahmad Khan5, Muhammad Shahzaib Tariq6, Muhammad Ali Raza6, Rana Usama Iqbal7 and Samia Zain8
...or, foraging preference, subspecies variations, monitoring approaches, and the need of preservation and conservation of these essential pollinators. To determine honey bee ecotypes and local species that are more resistant to pests, diseases, and pesticides, as well as to quantify the synergistic impacts of the probable causes of present colony loss, further research is required....
Yingying Wang, Jianyi Yu and Lichen Zhou*
...he most endangered tiger subspecies endemic to China, remain only a little more than 200 today, was susceptibly infected with Toxoplasma gondii. In this study, we used polymerase chain reaction to detect DNA, and enzyme-linked immunosorbent assay to test for T. gondii antibodies. Antibodies (S/P>0.31) to T. gondii were found in 20 (26.32%) of the 76 tigers, while all blood samples tested through nested PCR were negative. This is the first investigation of T...
Wen Xiong1*, Shengao Chen2, Dong Xie3, Qiang Wang4, Yanxia Li5, Lei Pan6, Xiaolei Ren7*, Wei Tang8*, Kang Chen9 and Ross N. Cuthbert10
...eshwater fish species or subspecies, belonging to 15 orders, 38 families and 105 genera in the Qiantang River, including species listed as among the ‘100 world’s worst invasive species. Therein, 24 species are endemic to China and 14 species are classed as endangered by the IUCN. We identify and discuss habitat loss, pollution, sand mining, over-fishing and non-native species as the major threats to freshwater fish biodiversity in this system. Thus...
Yongke Zhu1,2, Yun Fang1, Songhua Tang1, Yuan Gu3, Jinming Zhao4, Wolfgang Scherzinger5 and Yue-Hua Sun1*
...er, the knowledge of the subspecies of boreal owl (A. f. beickianus) in the coniferous forests at high mountainous altitude of the Qinghai-Tibet Plateau is still limited. We spent 10 years studying the breeding biology of A. f. beickianus in Lian Hua Shan Nature Reserve (Gansu Province), where we could first confirm the occurrence of this small forest dwelling owl in 1995. During breeding seasons (2003-2009, 2017-2019), we checked nest boxes and recorded the b...
Yongtao Xu1, Dandan Wang1, Xiaolong Hu1, Minling Li1, Ming Tang1, Wuhua Liu2, Jianwen Zhan2 and Weiwei Zhang1*
...kopschi) is one of three subspecies left in China. Diet analysis is one of the core contents in studying animal habitat requirements, and a study of their diet could provide valuable reference for species conservation and management. The metabarcoding trnL regions of the chloroplast genome from 30 fecal samples of Taohongling sika deer were amplified with universal primers and sequenced by high-throughput. We found that 28 out of 30 valid samples had a total o...
