Zijuan Li1,2, Rong Ma2, Muhammad Khan3*, Chenchen Liu2, Xiaolin Cui2 and Yongming Li1,2*
... of piceatannol in human osteosarcoma U2OS cells. Cell proliferation was measured using CCK-8 assay and colony forming assay. Flow cytometry was used to determine apoptosis and cell cycle profile. Western blot was used to measure the expression of various proteins. The data demonstrated that piceatannol inhibited growth and induced G2/M phase arrest and apoptosis. Further studies showed that piceatannol induces mitochondrial apoptosis as shown by Bcl-2 family ...
Junli Liu1, Tongfang Yang2 and Lisheng Wu3*
...eration and apoptosis of osteosarcoma cells through long non-coding RNA (lncRNA) LINC00857/microRNA (miR) -340. U2OS of osteosarcoma cells was treated with harringtonine at a concentration of 0.25, 0.5, 1, 2, 4 μg/ml. P21 and cysteine-containing aspartic protease 3 (Caspase-3) protein expression in U2OS cells were detected by western blot, thiazole blue (MTT) and plate cloning experiments to determine cell survival and co...
Hanan Fathy Hammouda1, Mohammad Mahmoud Farag2*, Mervet M.F. El Deftar3, Mohamed Abdel-Gabbar4, Basant M. Mohamed4
...ls (rBM-MSCs) and cancer osteosarcoma cells. The non-cellular in vitro bioactivity test was performed in simulated body fluids for periods 1, 3, 10, 20 and 30 d by measuring the calcium and phosphate ion concentrations by SEM/EDX analysis. While, the bioactivity of expanded and differentiated osteoblast cells derived from isolated rBM-MSCs by flowcytometric analysis was studied by histochemical staining with Alizarin Red and von Kossa to confirm the ost...
