Bacillus subtilis BML5 Isolated from Soil Contaminated with Poultry Waste has Keratinolytic Activity
Aqsa Imtiaz and Abdul Rehman*
...m amongst nine bacterial keratinase producing isolates were found. The BML5 isolate was identified as Bacillus subtilis based on biochemical characterization and 16S rRNA Ribotyping. B. subtilis BML5 showed maximal keratinase activity at 37°C and at pH 8. An increase in keratinase activity of B. subtilis BML5 was determined in the presence of Ca2...
Farha Deba1, Rubina Nelofer2 and Muhammad Irfan1*
...strains that can produce keratinase were isolated and identified from the abattoir region. A transparent, clear zone was evident in each isolate during the initial primary screening on skimmed milk agar plates. The secondary screening was then evaluated via submerged fermentation utilizing raw feathers as a substrate for keratinase production. On skimmed milk agar plates with streaked pure culture growth patterns, these seve...
MARIAM AHMED, YESRA ARSHAD & HAMID MUKHTAR*
...r and then evaluated for keratinase production by using raw feathers as substrate in submerged fermentation. Two isolates, showing maximum enzyme production, were identified as Bacillus species on their morphological and biochemical basis. Crude enzyme revealed that Bacillus sp. IIB-B5 produced maximum keratinase (28.13 U/mL) at pH 7.0, at 40oC after 72 h incubation. While Bacillus sp. IIB-B9 showed maximum enzyme production...
