Usman Waheed1,*, Humayoon Shafique Satti2, Muhammad Arshad3, Ahmed Farooq3, Abdul Rauf4 and Hasan Abbas Zaheer1,5
Muhammad Said1, Amjad Hussain Mirani1, Abdul Kabir*1,2, Muhammad Haris Raza Farhan3, Abdul Latif Bhutto1, Ghulam Shabbir Barham1, Khaleeq Ur Rahman Bhutto4, Muhammad Uzair1, Maaz Khan1
...c approaches, particular immunoassays, and infrared thermography, all of which provide quick findings. This article efforts primarily on the complex methods of mastitis detection, as identification of the etiological agents are vital to preventing mastitis in dairy cows.
Keywords | Mastitis, Diagnosis, Bovine Mastitis, Immunoassays, Diagnostic Techniques, Chronic Mastitis
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Characterization and Molecular Detection of Tomato spotted wilt Tospovirus Infecting Tomato in Egypt
Hayam S. Abdelkader1; Gamalat M. Allam1; T. A. Moustafa2; and M. El-Hanunady2
...as confirmed by Dot blot immunoassays. RT-PCR assays using primers complementary to the nucleocapsid protein gene (NPs) were used to detect two isolates of TSWV from Lycopersicum esculentum and Physalis peruviana plants. Total nucleic acids were reverse transcribed using Retrotool reverse transcriptase enzyme and the PCR reactions were performed for 30 min in a capillary thermal cycler. RFLP analysis of the PCR products was performed using Msel restricti...
