El-Sayed M. Abdelwhab, Jutta Veits and Thomas C. Mettenleiter
Avian Influenza H5N1 in Egypt: What we Know and What we have to Know?
...nied by mutations in the hemagglutinin (HA) protein which improved the binding affinity to human receptors but simultaneously retained its specificity for avian-receptors. Vaccines were applied nationwide to control the disease in poultry. Meanwhile, the viruses accumulated several point mutations in the HA immunogenic epitopes resulting in antigenic drift and the establishment of infections in vaccinated poultry. The Egyptian H5N1 viruses remain susceptible t...
Ahmed Samy, Wesam Mady, Naglaa M. Haggag, Samah H. Mohamed, Ebtissam N. AlShamy, M.K. Hassan
Sameera Akhtar1*, Muhammad Akram Muneer1, Khushi Muhammad1, Muhammad Yasin Tipu1, Muhammad Anees2, Imran Rashid1, Raza-ur-Rehman3 and Irshad Hussain1
...alysis of fusion (F) and hemagglutinin (HN) genes of NDV strain isolated from an outbreak in a peacock flock was undertaken. Proteolytic cleavage site of F0 showed a cleavage motif (112RRQKR↓F117), representative of a velogenic serotype. Moreover, based on F and HN gene nucleotide sequence analysis, the isolate clustered as genotype VII closely associated to those reported from Indonesia. Further sequence analysis of hyper...
Dildar Hussain Kalhoro1,2,, Shan Liang1, Muhammad Saleem Kalhoro2, Shoaib Ahmed Pirzado2, Nasir Rajput2, Muhammad Naeem2, Fahmida Parveen2 andYongjie Liu1*
.... Sequencing analysis of hemagglutinin (HA) and neuraminidase (NA) genes showed 93 to 99% similarity between each other. The CIV isolates shared high similarity (above 98%) to the H3N2 viruses from dogs in China. On the comparison of gene sequence four unique mutations were found in the amino acid of HA (A144T, R158K, D291N, L383F) and NA (T19A, V33L, V82A, S336N). The HA and NA genetic evolution analysis revealed that one isolate was most similar to the newly...
Evolutionary Analysis and Phylodynamics of Avian Influenza Virus H5N1 between 2015 and 2016 in Egypt
Osama Elshazly1, AbdelSatar Arafa1, Mohammed A. Rohaim2, Ismaeil M. Reda2 and Hussein A. Hussein2*
...ion methods. Full-length hemagglutinin (HA) sequences of six representative H5N1 isolates were analysed to study their genetic evolution followed by estimation of their evolutionary rates among different virus clusters. This analysis revealed a high evolution rate for clade 2.2.1.2. Additionally, analysis of selection pressures in the HA gene revealed a positive selection pressure. Deduced amino acid analysis revealed characteristic mutations at HA antigenic s...
Amany Adel, Wesam Mady*, Zienab Mosad, Fatema Amer, Asmaa Shaaban, Dalia Said, Marwa Ali Abdel Maged, Abdel-Satar Arafa, Mohamed Kamal Morsi, Mohamed Khalifa Hassan
Amany Adel, Wesam Mady*, Zienab Mosaad, Fatma Amer, Asmaa Shaaban, Dalia Said, Marwa Ali, Abdel-Satar Arafa, Mohamed Kamal Morsi and Mohamed Khalifa Hassan
Irfan Irshad1, Asim Aslam1,*, Muhammad Yasin Tipu1, Kamran Ashraf2, Beenish Zahid3 and Abdul Wajid4
...s clade. Analysis of the hemagglutinin (HA) gene sequences of the two AIV was performed and phylogenetic analysis reveals genetically closely-related to H9N2 viruses classified into Mideast group-B and sub-lineage B2. The two strains classified as LPAIV in poultry based on amino acid sequence at the proteolytic cleavage-site of the HA gene with PAKSSR/G. Our findings highlight the potential risk of ND and AI in poultry and continued active surveillance is need...
Hasaan Aziz*, Fariha Altaaf, Attia Bashir, Abdul Basit, Syed Anam Masood Gardazi and Farah Haider
...ly analysed the complete hemagglutinin gene of CDV from a male dog (14 months old) exhibiting clinically suggestive symptoms. With a characteristic amino acid residue for dog at Y549, sequence analysis showed geographically distinct pattern clustering the study strain to clade Asia-2 but clearly distinct from vaccine strains. Though limited and preliminary data, the findings enhance our knowledge towards CDVs circulating in Pakistan. Further studies are much n...
Mohamed. E. Taha1, Mohamed S. Ahmed2, Ahmed I. Ahmed2, Amany Adel3, Salama Rehab4,5*, Nabila Osman2**
... complete sequencing of hemagglutinin gene. Amino acid sequences showed that these isolates were identical to the Asian strain “A Quail-HongKong-G1-97” (91-92%), as well as the other Egyptian strains “A/chicken/Egypt/ME543V/2016” (99-100%), and “A/quail/Egypt/113413v/2011” (98-99%). Also, six of the N-glycosylation sites were identical to the Hong Kong isolate “HK/G1/97”, while the other two glycosylation sites...
Abdulaziz A. Alaqil
...e thickness against phytohemagglutinin injection, 44% in the anti-sheep red blood cells antibody titer, and more than 50% in T- and B-lymphocyte stimulation index, while recording a double increase in the heterophil to lymphocyte ratio. In contrast, the productive traits and immunological aspects were significantly (p < 0.05) augmented by PR supplementation to the broiler diets. Furthermore, PR supplementation successfully restored the broiler production an...
Farid S. Nassar1,2, Osama A. El-Sayed3, Saidi Ouassaf4, Ahmed O. Abbas1,2*
...(AB) titers and the phytohemagglutinin-wattle reaction (PHA-WR), respectively. It was observed that most of these measures showed a linear trend response to the rise in FS levels up to 30 g/kg of broiler diets. The FS30 group presented the lowest values of CORT and MDA by approximately 21% and 15%, respectively, and the maximum levels of AB titer and PHA-WR by 38% and 3-fold, respectively. These findings suggested that dietary supplementation with FS would be ...
