Nirbhay Kushwaha, Achuit K Singh, Brotati Chattopadhyay and Supriya Chakraborty
Recent advances in geminivirus detection and future perspectives
... cotton, pulses, papaya, cucurbits, okra etc.) causing enormous losses in the tropics, which provide ideal conditions for the perpetuation of viruses and the insect-vector. Despite concerted efforts to contain begomoviruses and their vectors, menacing disease epidemics caused by newly emerging or reemerging begomoviruses are becoming frequent and appearing even in new regions, previously free from such diseases. Techniques for geminivirus detection include bio...
Muhammad Tariq-Khan1*, Syed Zanib Ali Gardazi1, Abu Daud Ahmad Khan1, Muhammad Ilyas2 and Ishaq Ahmad3,4
Virulence and Distribution Trends of Root-Knot Nematode (RKN) Fauna on Summer Vegetables in District Bagh, Azad Jammu and Kashmir (Pakistan)
...mber, chilies, beans and cucurbits were found most frequently cultivated vegetables in the area. RKN was found on 80 fields, with 68 out of 82 surveyed locations with 72% field incidence. Okra was found with highest field infestation with 47.3% followed by cucurbits on 20% and tomato on 17.4% while zero infestation on chilies. Species were identified based on perineal pattern morphology. M. javanica was identified as predomi...
Muhammad Ibrahim Kubar1, Fahad Nazir Khoso1*, Imran Khatri1, Niaz Hussain Khuhro2 and Arfan Ahmed Gilal1
...y vegetables, especially cucurbits. To manage this notorious pest, the experiments were designed to evaluate the most effective method of pest control. The field experiments in bottle gourd, sponge gourd, Indian squash and bitter gourd were designed in Randomized Complete Block Design (RCBD) having 16 treatments with 5 replications each. Basically, there were five treatments; T1= Untreated control, T2 = Spinosad (Tracer), T3= Cue-lure, T4= Protein hydrolysate,...
Aly M. Abdel-Salam
...y-transmitted viruses to cucurbits include viruses in the Begomovirus, Crinivirus, and Ipomovirus genera. Induced symptoms are mostly of the yellow-type disease symptoms. Previous observation indicated that co-infection of cucurbits with these viruses was very common and may lead to severe problems in virus diagnosis. Objectives: The purpose of this study is to characterize biologically, serologically, and molecularly one Cr...
Shimaa A. Khalaf1 , Mohsen M. Elsharkawy2, Samir A. Sidaros2, Shawky A. El Kewey2, Ayman F. Omar2, Ali Hamed1
...eat to the production of cucurbits all over the world. The sterile fungus GU23-3 is a rhizosphere fungus conferring positive effects on plant growth and health..
Objective: The aim of this study is to evaluate the effects of GU23-3 on cucumber growth and resistance against PRSV..
Methods: Disease severity and virus titer were evaluated in cucumber plants treated with barley grain (BGI) or culture filtrate (CF) of GU23-3 under greenhouse...
Abdelhak Rhouma 1 Ibtissem Ben Salem 1 Mahmoud M’Hamdi 2 Naima Boughalleb-M’Hamdi 1
Volume 2, Issue 5 September and October 2018 Pages 85-100
...onballus responsible for cucurbits Monosporascus root rot and vine decline, is worldwide spread notably in Tunisia. The most appropriate strategies to suppress disease development are those able to reduce the ascospores population using eco-friendly approach treatments. Seven soilborne fungal isolates were tested in vitro (by dual confrontation technique) and in vivo in the greenhouse as potential bioagents against three virulent M. cannonballus isolates. In v...
Naïma Boughalleb-M’Hamdi1*; Najwa Benfradj1; Ibtissem Ben Salem1; Paloma Abad-Campos2
Novel Research in Microbiology Journal (2019), 3(1): 271-280
...g serious damages to the cucurbits production areas in Tunisia. The goal of this investigation was to study the genetic characterization of F. solani f.sp. cucurbitae Tunisian population. Ten isolates of F.s.c. (F.s.c.1 to F.s.c.10) collected from infected watermelon plants in numerous regions of Tunisia; were subjected to PCR identification using ITS1-ITS4 primers pair, and Inter-simple sequence repeat (ISSR) technique. Results obtained confirmed the presence...
Shrouk E.E. Farg1; Shafik D. Ibrahim2; Samir Mahgoub3*; Atef S. Sadik1; Mamdouh H. Abdel-Ghaffar1
Novel Research in Microbiology Journal (2024), 8(2): 2370-2392
...ic virus (ZYMV)-infected cucurbits compared to the healthy plants based on three molecular tools. When sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS- PAGE) analysis was used to determine the genetic variability of four cucurbits infected with ZYMV compared to the healthy ones, a total of 15 storable protein bands were recorded. The experimental results showed that DNA polymorphisms included 38 polymorphic DN...
