Sania Subhan Qureshi, Mian Saeed Sarwar and Zahir Shah
Sameera Akhtar1*, Muhammad Akram Muneer1, Khushi Muhammad1, Muhammad Yasin Tipu1, Muhammad Anees2, Imran Rashid1, Raza-ur-Rehman3 and Irshad Hussain1
...ell as in commercial and backyard poultry.
... 
Wesam Hasan Mohamed Mady1*, Bing Liu2, Dong Huang2, Abdel Satar Arafa1, Mohamed Khalifa Hassan1, Mona Mehrez Aly1, Pucheng Chen2, Yongping Jiang2* and Hualan Chen2
... for both commercial and backyard poultry production sectors. Owing to public health risk, effective control of the virus in imperative. DNA vaccination is a promising new approach for the prevention and treatment of many viral diseases because of its ability to induce both humoral and cellular immune responses against antigens encoded by recombinant DNA. The goal of this study was to construct Egy-H5 plasmid DNA-based vaccine against a currently circulating a...
Evolutionary Analysis and Phylodynamics of Avian Influenza Virus H5N1 between 2015 and 2016 in Egypt
Osama Elshazly1, AbdelSatar Arafa1, Mohammed A. Rohaim2, Ismaeil M. Reda2 and Hussein A. Hussein2*
...including commercial and backyard flocks, in five different locations in Egypt between the years of 2015 and 2016. All samples were screened using virus isolation, as well as antigenic and molecular detection methods. Full-length hemagglutinin (HA) sequences of six representative H5N1 isolates were analysed to study their genetic evolution followed by estimation of their evolutionary rates among different virus clusters. This analysis revealed a high evolution...
Muhammad Huzaifa Mehmood1, Muhammad Ahmad Iqbal2, Muhammad Daood1, Muhammad Rizwan Tariq3*, Khubaib Ali3
...d for meat production in backyard farming system in rural areas of Pakistan. The quality of meat depends upon the nutritious value, texture profile, taste and flavour. Mostly dieticians recommend rabbit meat due to low in caloric value then other traditional meats. To enhance the quality of rabbit meat oat is used as feed for rabbits, because oat is good source of dietary ingredients. In this study 2-4% oat seeds were used to investigate their dietary response...
Wesam Mady*, Nahed Yehia, Mohammed Hassan Elhussieny, Azhar Gaber Shalaby, Moataz Mohamed El Sayed, Neveen Rabie Bakry, Asmaa Shaaban, Abdel Satar Arafa, Afaf Abdel Baset Mahmoud and Wafaa Mohammed Hassan
... both the commercial and backyard poultry production sectors. The aim of this work is to study the Situation and molecular characterization of HPAIV (H5N8) in backyard poultry production sector in Egypt. In this study, a total of 7505 samples of tracheal swabs representing 1180 backyard poultry flocks were collected based on the surveillance conducted by the GOVS (General Organization for ...
Muhammad Furqan Shahid1*, Tahir Yaqub1, Muhammad Yasin Tipu2, Asim Aslam2, Saima Yaqub1, Aziz-ul-Rahman3, Muzaffar Ali1
...solated from commercial, backyard poultry and fancy birds (partridge). Commercial broiler birds were infected experimentally at the dose of 106 EID50 by using these three isolates. Results revealed that onset of clinical signs was started at 2nd day post infection (DPI) followed by severity in redness and swelling of eyes, sneezing and lacrimation at 3rd DPI that lasts up to 5th DPI. Cross reactivity of an...
Amany Adel1, Abdelsatar Arafa1, Hussein A. Hussein2 and Ahmed El-Sanousi 2
...th live bird markets and backyard populations. NS1 gene was amplified, sequenced and the phylogenetic analysis of the obtained sequences revealed deletion of 5 amino acids at positions 80 to 84 in the NS1 protein along all Egyptian isolates under study. Ala149, which is a pathogeneicity marker for interferon antagonism was found in the studied indicating capability of these viruses to transmit between different avian species. PL motif ESEV was found in 6 isola...
Riham H. Bassam, Hussein A. Hussein, Mohamed M. Amer, Basem M. Ahmed and Ahmed A. El-Sanousi
... layer hens of breeders, backyard chickens and ducks for serosurveillance and
molecular characterization. Also; sequence analysis of the same IBV-positive samples.
Results: flocks had shown high discrepancy of CV% values with regard to ELISA results against IB
virus. Out of 60 flocks tested; 12 were positive to the presence of IBV by rt-RT-PCR despite the fact
that the detected flocks had a history of previous IB...
Riham H. Bassam, Hussein A. Hussein, Mohamed M. Amer and Ahmed A. El-Sanousi
...v>breeders, laying hens, backyard chickens and ducks. Tracheal swabs were collected from flocks raised
in different governorates in Lebanon.
Results:Testing of samples with rt-RT-PCR revealed that 6 flocks reared in 4 Lebanese governorates
were positive (CT ranged from 20.42 to 33.86). A Positive sample with CT 20.42 was tested and
partially sequenced and submitted to GenBank, acquired accessions number MH266696 ...
Eman H. Mahrous1, Mohamed. W. Abd Al -Azeem2, Faisal A. Wasel1, Waleed Younis2*
... respiratory signs) from backyard rabbits at different localities in Sohag governorate, Egypt. All samples were submitted for PCR test and other conventional methods of identification. Recovery of P. multocida isolates of diseased rabbits from lung, liver, and heart were 23 (46%), 11 (22%), 13 (26%), respectively, with a total incidence of 47 (31.3%). Biochemical reactions proved that 20 (42.5%) out of 47 isolates had the typical biochemical properties of P. m...
Md. Taimur Islam1*, Mirza Mienur Meher2, Anas Bin Harun3 and Md. Golam Haider1
...yer, broiler, sonali and backyard chicken. AI outbreak is observed every year since its first report in 2007 in Bangladesh. This virus has mutated several times and thereby changed its genetic clades. The prevalence of ND is highest in October and in 0-3 months aged chickens. AMPV generally infects the upper respiratory tract of poultry, which has been isolated for the first time in Bangladesh in 2016. Alternatively, adult chickens are more susceptible to IB a...
Genevieve R. Tabon1,2, Noraine P. Medina2, Mary Rose D. Uy-De Guia3, Claro N. Mingala2,3*
... commercial scale but in backyard production. The aim of the study was to assess the allelic variation in 30 Philippine native pigs (PNP). Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) was done to evaluate the genotypes of ESR1 gene. Target gene was amplified and then subjected to RFLP using PvuII restriction enzyme. Results showed that all of the samples were found to be of AA genotype which is not the favorable genotype accord...
Muhammad Tariq Navid1,2*, Mian Muhammad Awais2*, Muhammad Irfan Anwar2 and Masood Akhtar2
...investigate asymptomatic backyard poultry birds for presence of AIVs. For this purpose, a total of 213 birds were randomly selected and from each bird sera, oro-pharyngeal and cloacal swab samples were collected in sterile containers. About 13.61% of the samples were found seropositive by using commercially available ELISA kit. The supernatants from oro-pharyngeal and cloacal swabs of the seropositive samples were separated and divided into two segments; one w...
Syeda Fakhra Waheed1, Asim Aslam1*, Muti-ur-Rehman Khan1, Kamran Ashraf2 and Beenish Zahid3
...f 50 tissue samples from backyard chicken flocks presenting suspected symptoms were collected during February 2020 and March 2021 for genetic characterization, followed by phylogentic analysis. A total of 4 isolates were sequenced based on partial VP1 and hyper-variable region of the VP2 genes simultaneously. According to phylogenetic analysis, the study isolates genotype A3B3 were identified as predominant strains in country backyard<...
Asmaa A. M. Abdel-Ghany1, Ahmed N. El Taweel2, Yassmin Moatasim2, Nagwa S. Ata1, Amany Adel3, Ayman Hany El-Deeb4, Ahmed Kandeil2, Mohamed A. Ali2*, Hussein Ali Hussein4*
...winter of 2016. However, backyard poultry production sector is commonly found in rural areas in Egypt with biosecurity deficiencies and could play a key role in epidemiology of AIVs in Egypt. Thus, the aim of this study was to determine the prevalence of AIVs in backyard ducks and geese in Assuit governorate, Egypt during 2018, and to study the genetic features of detected AIVs. A total of 245 oropharyngeal and cloacal swabs...
Rameez Raja Kaleri1,2*, Hubdar Ali Kaleri1, Nazeer Hussain Kalhoro5, Raza Ali Mangi3, Ghulam Mustafa Solangi4, Deepsh Kumar Bhuptani6, Sher Muhammad Khosa1, Abdul Wahid Solangi1, Ayaz Ali Lashari7 and Sheva Dari1
...terization of indigenous backyard poultry birds in Tando Allahyar. For this purpose, total 470 birds with 240 male and 230 females were brought under the study. Their phenotypic characteristics were collected thorough observing by the researchers from different UCs of district Tando Allahyar. The data regarding quantitative traits were observed using the measuring tap mentioned in the (FAO) manual. The findings of our study 86.69% out of the 470 birds recorded...
Omarl , Dalia M.; El-Ibiaryl , Elham A.; Sadik2, Atef S.; Abdel-Ghaffar2, Mamdouh. H. and Othman2, Badawy A.
...ry production sector and backyards in Egypt and resulted dramatic economic losses to the poultry industry of Egypt and continue to pose a serious threat to public health. The present study was designed for detection and isolation of Avian Influenza Viruses (AIV) circulating among poultry since their first detection in 2006. Tracheal and cloacal swabs were taken from the freshly dead birds. Till now (2010) the tested swab samples were inoculated into the allant...
Arafa, A ; El-Kanawaty, Z; Hassan, M.K; Anwar, H.K and Mona M.Aly
...ors as well as rural and backyard level in Egypt. This outbreak also was extended to include the zoo birds In Giza zoo. This study records the isolation and characterization of H5Nl virus from different species of the zoo birds and also studies the immune states of the vaccinated birds after application of H5Nl vaccine for the first time one month after the introduction of early outbreaks of 2006. Viral diagnosis was based on direct detection of viral RNA by r...
Madbouly, H.M; Orkhan, M.H**; Nagwa El-khoty
...red in poultry reared in backyards or roofs of homes in Qaluobia & Giza governorates as many notifications were received by the General Organization of Veterinary Services (GOVSs) in February 2006. The disease was spread and recorded in chickens (foreign breeds , Balady , and hybrids ) , turkeys, ducks , geese and wild birds (from Giza Zoo) in 20 out of 26 (76.9 %) of the Egyptian governorates within few weeks. The number of confirmed farm cases was 840 fa...
Arafa, A.; Kanawaty, Z.; Abdclwhab. E. M.; Hassan, M.K.; and Aly M.M.
... biosecurity measures in backyard birds and possibility of continuous contact with wild migratory birds make the vulnerability of backyards to avian influenza much easier than commercial poultry farms. Avian Influenza subtype H7 was isolated from wild migratory ducks in El-Abassa Lake at El-Sharkia Governorate in 2007. To avoid repeating the scenario of H5N1 introduction to Egypt through wild migratory ducks to Egyptian poul...
Kunlayaphat Wuthijaree1, Pattaraporn Tatsapong1, Sukanya Yung-Rahang2, Prayad Thirawong2, Koonphol Pongmanee2*
...f the health problems in backyard chickens and is detrimental to productivity performance. This study evaluated the prevalence and average number of gastrointestinal parasites per chicken (worm burden) in Thai indigenous chickens (Gallus gallus domesticus). A total of, 229 chickens (113 males and 116 females) were investigated in 3 selected districts in central Thailand. Chickens were raised under extensive backyard conditio...
Mirwaise Khan1, Rubab Maqsood1, Hamad Bin Rashid2, Shakera Sadiq Gill1, Muhammad Noman1, Rafia Akram1, Muhammad Sajid Hasni1, Haroon Rashid3 and Mamoona Chaudhry1*
...f 240 blood samples from backyard chickens were collected and were tested for antibodies against Avian Influenza Virus (AIV) (H9N2) by hemagglutination inhibition test according to OIE. It was found that out of 240 collected blood samples, 140 (58.3%; 95% CI: 50.43-69.60) were positive for AIV (H9N2). The findings of the present study indicated that AIV (H9N2) were endemic and widely distributed in backyard poultry in differ...
Walid Elmonir1*, Dalia Abdeltawab1, Hanem El-Sharkawy2 and Rasha N. Zahran3
...onella in commercial and backyard egg production systems in Kafrelsheikh Governorate, Egypt. A total of 610 samples (200 egg shells, 200 egg contents, and 210 environmental samples) were collected from 10 commercial layers farms and 170 samples (50 egg shells, 50 egg contents, and 70 environmental samples) were collected from 10 backyard layers flocks. The overall prevalence of Salmonella was 4.1% for each production system....
