Apple chlorotic leaf spot virus (ACLSV) isolated from apple trees was successfully transmitted by dagger nematodes (Xiphinema spp) It was transmitted to bait plants grown in soil containing Xiphinema spp. nematodes, The virus was identified according to host range, mode of transmission, particle morphology, serological tests, and SDS-Polyacrylamide gels. Purification of ACLSV was performed using bentonite/polyethylene glycol. Electron micrograph of purified virus preparation revealed flexuous filamentous virus particles of 700 nm length and 12 nm wide. The average yield was 1.14-1.7mg/100 g tissue of Chenopodium quinoa Willd. Purified virus preparation was used for rabbit immunization. The polyclonal antibodies raised against the local isolate of ACLSV had a specific titer of l: 8000. The concentration of IgG and IgG conjugate with alkaline phosphatase was l: 1000. The prepared antiserum was used for detection Of ACLSV using ELISA dot immunobinding assay (DIBA). The presence of the virus was checked in blossom parts. (Petioles, stigma, and flower cup).