The objective of the present study was to evaluate an enzyme linked immunosorbent assay (ELISA) based kit for detecting foot and mouth disease (FMD) antibodies using a native FMDV strain (O1. Aga 93). Results were compared to those of serum neutralization test (SNT) and 3CD ELISA Kit. Sensitivities of the ELISA Kit and 3CD ELISA were 94% and 91%, respectively. Rapid confirmation of the presence of FMDV antibodies through the developed ELISA Kit may have a significant impact on initiating emergency control of FMD outbreaks.