sp. Issue 2010

Inheritance of Resistance to Bean Yellow Mosaic Virus in Faba Bean Plants

El-Bramawyl, M . A. S. A, El-Beshehy, E. K. F.'

Bean yellow mosaic virus (BYMV) is one of a devastating pathogen that reduces faba bean crop production in Ismailia. Resistance is limited and resistance-breaking isolates are becoming problematic. Effective BYMV disease control strategies will come from a bet…

Detection of xanthomonas vesicatoria Phages in Infected Tomato Plants

Eisa, Nawal A, Abd El-Ghafar, N. Y. Abd EL-Mageed, M.H, Mohamed et al.

Different Phages parasitizing Xanthomonas vesicatoria (the causal agent of the bacterial spot disease of tomato) were isolated from infected leaves of tomato and from tomato rhizosphere soil, using enrichment technique. The phages produced plaques (4-5mm, diam…

Post-Distribution Survey for Potency of Live Attenuated Oral Polio Vaccines (OPV) used in Sudan Northern States Peripheral Children Immunization Clinics

Elamien, Mohammed, Kotob, Nahla S, Elsayed, Eltayeb A et al.

National immunization days (NIDs) started in 1994 in Sudan by using trivalent OPV (topV) and in the beginning of 2010 bivalent OPV (bOPV) was permitted to be use. Since OPV is manufactured from a highly thermolabile vaccine strain of poliovirus, it should be s…

Field Diagnosis of Avian Influenza 115N1 Virus M Egypt Using Rapid Immunochromatographic Diagnostic Tests

Ibrahim l, Madiha Salah, Ikuta, Kazuyoshi

Rapid on-spot testing for influenza virus can greatly assist in the clinical management decision. In Egypt, where avian influenza A virus H5Nl is now endemic and human infections are on the rise, rapid H5N1 detection in infected poultry is essential for contro…

Comparative Detection of 1-15N1 Avian Influenza Virus using Conventional RT- PCR and Real Time RT-PCR

Hagag, N.M.*•, Arafa, Shalaby, M. A. ** El-Sanousi, A. A et al.

Highly pathogenic avian influenza (HPAI) caused by influenza A H5N1 virus, poses a significant threat to the poultry industry and humans in Egypt. Since it was first recognized in 2006, the disease has become enzootic in poultry throughout Egypt and still circ…

Epidemiological Diagnosis of Foot and Mouth Disease (Pmi) among Cattle in Sharkia and Kafr El Sheikh Governorates

Raof, Amal M. A, Haleem, Iman Y, Aly, Nawal M et al.

Foot-and-mouth disease (FMD) is one of the most contagious diseases affecting wide range of host species with variable severity and decreased productivity. Non-structural protein (NSP) 3ABC antibody is considered to be the most reliable indicator of present or…

Serological and Molecular Identification of Some Isolated Avian Influenza Viruses During Outbreaks in Egypt

Omarl, Dalia M, El-Ibiaryl, Elham A, Sadik, Atef S et al.

In mid-February 2006, an outbreak of Highly Pathogenic Avian Influenza (HPAI) H5Nl affected the commercial poultry production sector and backyards in Egypt and resulted dramatic economic losses to the poultry industry of Egypt and continue to pose a serious th…

Establishment of Dot-Blot Hybridization for Diagnosis Of Bovine Ephemeral Fever Virus in Egypt

Zaghloull, A.H, Mahmoud, Amal, Hassanl, H.Y et al.

Bovine ephemeral fever (BEF) is an arthropode borne rhabdovirus, which causes a disabling febrile infection of cattle and water buffalo. A sever outbreak was recorded in Egypt during 2000 and 2001. Using RT PCR, a primer designed to amplify 438 nucleotide frag…

Virological and Serological Investigations on Avian Pneumovirus in Turkeys and Chickens

Madboulyl, H. M, Tamami, S.M, Abd Elmoneml, A.S et al.

Avian Pneumovirus (APV), which is termed turkey rhinotracheitis (TRT), is successfully isolated from 5 out of 8 samples of nasal discharge and 10% homogenates of nasal sinuses collected from young turkey poults of unvaccinated flocks by passage on SPF-ECE of 5…

Comparison of the Efficacy of Some Regimes of Vaccination for the Control of VVIBDV in Broilers

Abd El-Razakl, A.G, AboElkhair

Two vaccination programs for infectious viral disease (IBD) were compared in broiler chicken with maternal immunity, placed in tow farms, A and B. IBD immune complex vaccine was administered by subcutaneous route, at the hatchery into chicks of farm A at age o…

Partial Characterization of an Isolate of Cucumber Mosaic Virus from Ismailia Governorate

Elbeshehy, E. K.F, Sallaml, A.A.A

Cucumber mosaic virus (CMV) was isolated from naturally infected cucumber plants Cucumis sativus L. grown in various garden and greenhouses of Ismailia Governorate, Egypt exhibiting systemic mosaic, blistering, fruit malformation and stunted plant growth and i…

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Biological Control of Cucumber Mosaic Virus by Certain Local Streptomyces Isolates

El-Dougdoug, Kh.A, Ghalyl, M.F, Tahal, M.A

An antiviral producing• Streptomyces species were isolated from soil rhizosphere in Zagazig province of Egypt. In order to identi$r the Streptomyces strains, morphological, physiological, biochemical and antagonism testes were performed. The Egyptian isol…

Characterization and Elimination of A TMV Isolate Infecting Chrysanthemum Plants in Egypt

Nassarl, Entsar A, El-Dougdoug, Kh. A, Osmanl, M.E et al.

An Egyptian isolate of a Tobamovirus was isolated and identified from Chrysanthemum cultivation in Egypt. Both biological, serological and sequence analysis of the coat protein gene demonstrated that the virus represents an isolate of the Tobamoviridae Family.…

Genetic Stability in Tissue Culture-Derived Banana Virus Tested using Thiouracil

Aboud, K. A, Gomaa, Hanaa H. A, El—Taholowy, Mohage et al.

Banana bunchy top virus (BBTV) is a destructive viral disease in many countries including Egypt. It causes severe economic losses because infected banana plants produce no fruit. -The tissue culture approach was used to permit the recovery of BBTV-free plantle…

Molecular Characterization and Bioinformatics Analysis of Viroid Isolate Associated with Citrus Gummy Bark Disease in Egypt

Sofyl, A.R, Soliman, A.M, Mousal, A.A et al.

This work reports the characterization of Hop stunt viroid which has been isolated previously in Egypt from sweet orange infected with gummy bark disease namely Citrus viroid Il. The native structure of mature, circular forms of the gummy bark agent was detect…

Incidence of Fruit Trees Viroid Diseases by Tissue Print Hybridization in Egypt

El-Dougdougi, Kh. A, Dawoud, Rehab A, Rezk, A.A et al.

Direct tissue printing on membranes has been applied on a large scale for an initial detection of CEVd, HSVd and PLMVd in fruit trees in Egypt. CEVd was detected mainly in sweet orange trees and occasionally in grapevine and mango. 'Ihe principal characteristi…

The Limiting of Climatic Factors and Predicting of Suitable Habitat for Citrus Gummy Bark Disease Occurrence Using GIS

Gummy bark disease is a disorder of sweet orange on sour orange rootstock in Egypt. There is an importance for hot-growing temperatures to symptom development of citrus viroids. The geographical distribution of the gummy bark disease in some world countries de…

Cytopathic and Histological Effects of Infected Phthorimaea Operculella Zeller (Potato Tuber Moth) with Granulosis Virus (POGV)

Mohamedl, Shimaa D, Ghareeb, El-Dougdoug, K. A, Bekheitl et al.

The potato tuber moth Phthorimaea operculella is a potato major pest in Egypt. Larvae of P.operculella were inoculated with a granulosis virus (PoGP) showed different cytopathic effects . PoGV induced characteristic symptoms of slow motion, white milky appeara…

Molecular Characterization and Efficacy of Spodoptera Exigua Nuclear Pol Yiiedros'is Virus

El Sayed l, Yasmein A, Ghareeb, El-Dougdoug, K. A, Bekheetl et al.

The beet army worm Spodoptera exigua (Hubner.) is affected by nuclear polyhedrosis virus, the disease specific polyhedral virus particles was found to be associated with the beet army worm (BAW). Inoculation of SeNPV into S.exigua induced characteristic sympto…

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Partially Nucleotide Sequence and Secondary Structure of Chrysanthemum Stunt Viroid Egyptian Isolate from Infected Chrysanthemum Plants

El-Dougdoug, Kh.A, Rezk, A.A, Dawoud, Rehab A et al.

Chrysanthemum stunt viroid Egyptian isolate (CSVd-EG) was isolated from infected Chrysanthemum plants. It is a member of Pospiviroidae. In order to study the structure of CSVd-EG, it was reverse transcribed in total RNA from infected leaves and then amplified…

Engineered Norovirus-like Particle as a Vaccine Candidate

Shawki, Khaled K, Carter, M.J, Alnashar, Nariman M et al.

Noroviruses account for the bulk of infectious gastroenteritis in adults worldwide. Although Norovirus gastroenteritis is self-limiting, the economic burden of infection is considerable and uncontrolled manifestations can result in death. Noroviruses form an a…

Molecular Variation of Potato Virus Y Isolated from Egypt

El-Absawy, E.A, Mahmoud, Amal, Hemeida, A.A et al.

Potato virus Y (PVY) is one of the most important viruses affecting cultivated potatoes in Egypt. Different potato plants were collected from an experimental station in Giza Governorate, Egypt and were tested using RT-PCR. PVY was amplified using primers repre…

Genetic Variations in A Conserved 5’-Untranslated Region of Hepatitis C Virus Isolated from Egypt

Hemeida, A. A, Osman, El-Shahat, Mohamed, Hashem et al.

Ninety-two positive samples for anti-HCV antibodies were selected from out patients of Center of Cardiac and Digestive System, Sohag, Egypt aged from 20 to 55 years. Then, confirmed by Reverse Transcription Polymerase Chain Reaction (RT-PCR) for HCV. The confi…

Molecular Variability of the S Gene of Hepatitis B Virus in Egypt

Elkalamawyl, I.M, Elhddadl, Swelim, M.A, Hamdy et al.

Two novel mutants of the hepatitis B virus Surface antigen (HBsAg) were characterized. The mutants were isolated from asymptomatic patients who were found to be persistently positive for both I-BsAg and anti-hepatitis B surface antibody (anti-HBs) and a long-l…

Molecular Characterization of Enzootic Camelpox Virus in the Eastern Kingdom of Saudi Arabia

Yousifl, Ausama A, Al-Naeem, Abdelmohsen A

Camelpox virus (CMLV) belongs to the genus Orthopoxvirus (OPV) of Poxviridae. CMLV causes clinical disease in camel populations worldwide. CMLV has been known in Saudi Arabia for centuries. However, no attempts have been made to characterize the endemic CMLV g…

Serodiagnosis of Foot and Mouth Disease (FMD) Virus for Differentiation between Naturally Infected and Vaccinated Cattle and Buffaloes

Hassanein, Suzan A, Abd El-Wahab, Wafaa, Eweis, Moustafa et al.

FMD is a highly contagious viral disease of all cloven-footed animals and widely distributed all over the world. In this study 465 serum samples were collected from 3 Nile delta governorates (Behaira, Mounofya and Kafer El-Sheikh) during 2009. The samples were…

Effect of Two Biotic Inducers on Salicylic Acid Induction in Tomato Infected with Cucumber Mosaic Cucumovirus

Mahdyl, A.M.M, Hafezl, M.A, EL-Dougdoug, Kh. A et al.

To explore the inhibitory effect of two biotic inducers on tomato plant against Cucumber mosaic cucumovirus (CMV), salicylic acid (SA) activities were determined in the inoculated and non-inoculated tomato plants. Identify of CMV was confirmed by some differen…

Lumpy Skin Disease Virus Identification in Different Tissues of Naturally Infected Cattle and Chorioallantoic Membrane (CAMS) of Emberyonated Chicken Eggs Using Immunofluorescence, Immunoperoxidase Techniques and Polymerase Chain Reaction

El-Kenawy, A. A, El-Tholoth, M. S

Lumpy skin disease virus (LSDV) was detected in 23sampIes collected from clinically diseased and slaughtered cattle showed clinical signs believed to be LSD. These samples include 7 skin lesions and 16 internal organs (lymph nodes (6), lung (4), kidney (3) and…

Enhancing Effect of Ginseng Stem-Leaf Saponins (GSLS) on the Immune Responses in Vaccinated Calves with Fmd Bivalent Vaccine

Rizk, Sonia A, El-garf, Eman M, Talaat, Abeer A

A comprehensive sero-immunological studies were conduced to reveal the adjuvant's effect of Ginseng Stem-leaf Saponins (GSLS) on the immune response of gel adjuvanted Bivalent Foot and Mouth Disease (FMD) vaccinated calves. This study conducted in two calve gr…

Curcuma Longa for Protecting Chicks against Newcastle Disease Virus Infection and Immunosuppressive Effect of Marek's Disease Viral Vaccine

Madbouly, H.M, Saif, M.A, Hussein, A.S

A total of 300 one day old Hubbard chicks were divided into 6 groups (GI-G6: 50 chicks/each) The Gl(control neither vaccinated nor treated), G2 vaccinated with NDV, G3 (vaccinated with MDV Rispen strain), G4 (vaccinated with MDV and NDV) , Group 5 vaccinated w…

The Effect of Some Electrolytes on the Susceptibility of BHK to FMD Virus

Mansour, Abeer E.M, Khodeir, M.H, Hussein, A.M.H.•

Three batches of foot and mouth disease virus (FMDV) type O suspension were prepared on 24 hours previously seeded baby hamster kidney (BHK21) cell culture. Each batch was composed of 15 roller cell culture vessels where the first batch represents the blank vi…

Application of Epitopes Prediction for Antibodies Production against Potato Leaf Roll Virus

Bakr, Youssef M, Nour El-Din, Hanan A, Abd EL-Wahed, Waiel F et al.

Epitopes prediction plays a vital role in the development of antibodies production and immunodiagnostic tests. This paper is focused on building models for predicting linear B-cell epitopes based on support vector machine (SVM) and subsequence string kemel. Th…

Molecular Characterization of Onion Yellow Dwarf Virus (Garlic Isolate) With Production of Virus-Free Plantlets

Soliman, Ahmed M, Mahmoud, Sabry Y. M, Dawood, Rehab A

Garlic samples showing symptoms of onion yellow dwarf virus (OYDV) were obtained from previous study and tested by indirect-enzyme linked immunosorbent assay (I-ELISA), transmitted to Chenopodium amaranticolor and then confirmed by immunosorbent electron micro…

Virological Evaluation of Drinking Water in Egypt

Azzami, Mohamed 1, Hakiml, Aya Salah El-Din, Othman, Badawi A et al.

This study aims to evaluate the inlet and outlet water of the Nile River at El-Rayah El-Menofy in three drinking water stations during the four seasons at 2007-2009. The water samples were ultrafiltered to concentrate coliphages, enteric and H5Nl viruses. Coli…

Three Viroids Frequency Naturally Infecting Grapevine in Egypt

Nasr-Eldin, M. A, El-Dougdoug, K. A, Othman, B. Ahmedi et al.

Three viroids: Citrus exocorlis viroid (CEVd), Hop stunt viroid (HSVd), and Potato spindle tuber viroid (PSTVd) can be detected in naturally infected grapevine by biological indexing and molecular methods. In the present study, direct tissue-printing and dot-b…

Impact of Polyethylene Glycol on Newly Established Ovarian Cell Line (Sl-Omi) from Leaf Worm of Spodoptera Littoral's and on Viral Infection

Khamiss l, Omaima, El Helaly, Alexandra, Abol Ela, Said

New cell line from the Egyptian cotton leaf worm ( Spodoptera littoralis ) originated from the insect ovaries tissues was established, cloned and maintained as contentious cell line. Nine serial dilutions of Polyethylene glycol (PEG) were prepared from mother…