A comprehensive sero-immunological studies were conduced to reveal the adjuvant's effect of Ginseng Stem-leaf Saponins (GSLS) on the immune response of gel adjuvanted Bivalent Foot and Mouth Disease (FMD) vaccinated calves. This study conducted in two calve groups; goup (A) vaccinated subcutaneously with bivalent Alhydragel adjuvanted (30) % FMD vaccine, while group (B) vaccinated subcutanously with bivalent FMD vaccine adjuvanted with both Alhydragel and GSLS (10 mg/dose). The humeral and cellular immunoresponses were monitored in different tested groups that received the gel adjuvanted vaccine and the Alhydragel-GSLS adjuvanted vaccine. Results indicated that the higher immune responses were found in calves vaccinated with Alhydragel-GSLS adjuvanted vaccin up to 24 week while with Alhydragel alone was only up to 18 week.