Three batches of foot and mouth disease virus (FMDV) type O suspension were prepared on 24 hours previously seeded baby hamster kidney (BHK21) cell culture. Each batch was composed of 15 roller cell culture vessels where the first batch represents the blank virus while the second batch was treated with 1ml of 1 mM calcium chloride (CaC12)/bottle in the inoculum's medium and the third batch received 1gm of mono basic sodium glutamate (MSG) /liter. Titration of individual bottles and pooled harvests of FMDV and complement fixing antigen revealed that CaC12 and MSG induced higher values than that obtained with the blank control batch where Ca C12 potentially facilitate the adsorption and penetration of FMDV to BHK cells increasing the virus attachment to the cell receptors. On the other side, MSG enhanced cell 1 division due to its of amino acid content and accordingly increased the available cell number for virus replication. There will be a need to more research to investigate the effect of CaC12 and MSG on the immune response of vaccinated animals with FMD vaccine including such materials and their effect on the vaccine stability.