Issue · 2004
Vol.1, Iss. 1, Pages 1-564
19 articles · pp. 1-564 · All issues →
Research ArticleMolecular Characterization of Potato Spindle Tuber Viroid Egyptian Isolate
E. K. Allam, Kh. A. El-Dougdoug, M. A. Amer, A. A. Abou-Zaid, S. M. Amin
Potato spindle tuber viroid (PSTVd) was detected in potato plants grown in Egypt using return-polyacrylamide gel electrophoresis (R-PAGE) and molecular techniques. The method involved the extraction of circular nucleic acids followed by electrophoretic analysi…
4 Apr 2023- Research Article
Molecular Cloning and Expression of the Coat Protein Gene of Plum Pox virus El-Amar Strain in E. coli
M. A. Abou El- Nasr, Kh. A. Dougdoug, Hayam S. Abdelkader, Rehab A. Dawoud
Plum pox potyvirus (PPV), the causal agent of Sharka disease of Prunus inflicts severe crop losses in affected El-Amar area. The virus isolated from EL-Amar apricot trees, was propagated on apricot healthy seedlings. Degenerated oligonucleotide primers were de…
4 Apr 2023 - Research Article
Cloning, Expression and Nucleotide Sequence of Coat Protein Gene of an Egyptian Isolate of Potato Virus y Strain NTN Infecting Potato Plants
M. A. Amer, M. H. El-Hammady, H. M. Mazyad, A. A. Shalabyl, F. M. Abo-El-abbas
Reverse transcription- polymerase chain reaction (RT-PCR) amplified product of the expected size of 801 bp was cloned into the Pinpoint Xa-l protein expression vector. The accury of each PCR amplified PVY CP gene was tested by PCR, restriction analysis, and tr…
4 Apr 2023 - Research Article
Nucleotide and Amino Acid Sequences Aompatison between Two Strains of barley Stripe Mosaic Hordeivirus (BSMV)
Om-Hashem M. EI-Banna, M. A. S El-Kady, E. A Salama, Salwa N. Zein
Nucleic acid sequence comparison between the Egyptian strain (Gl19) and the American strain (ND 18) of Barley Stripe Mosaic virus (BSMV) revealed a high degree of sequence similarity of γ RNAs comparing to ß RNAs of both strains. On the Other hand,…
4 Apr 2023 - Research Article
Assessment of Hepatitis E Viral Infection and its Effect on Egyptian Pregnant Mothers
A. A- E. Abo-Elkhier, K. S. E. Abdel-Wahab, M. A. Ali, M. A-H. El-Sayed, N.A-K. Saleh, A. A. Atef
The current study was performed to determine the prevalence of HEV in Egyptian pregnant women. and to estimate the risk or prenatal transmission of the virus and its effect in infected pregnant women. 120 aborted women between 17-36 years old and 120 women age…
4 Apr 2023 - Research Article
Production of Polyclonal Antibodies against the Recombiant Citrus tristeza virus Coat Protein Expressed in Escherichia coli
Hala A. Amin, A. Barakat, A.A. Abou Zeid
Early diagnosis based on immunoenzymatic techniques requires significant amounts of immunoreagents. Hence, using directional cloning technology, a 38-KDa fusion protein containing a complete coat protein (CP) gene or Citrus tristeza virus (CTV) encoded by the…
4 Apr 2023 - Research Article
Detection of Grapevine Fanleaf Virus Capsid Protein Gene by RT-PCR and DNA hybridization
E. K. Allam, B. A. Othman, Hayam S. Abdelkader, Noher A. Mahmoud
Grapevine fanleaf virus (GFLV) was isolated from symptomatic grapevine (Vitis vinifera L.) leaf samples obtained from El Kalubia Governorate. Three out or 25 samples were positive for GFLV by DAS-ELISA using specific polyclonal antibodies raised against purifi…
4 Apr 2023 - Research Article
Characterization and Molecular Detection of Tomato spotted wilt Tospovirus Infecting Tomato in Egypt
Hayam S. Abdelkader, Gamalat M. Allam, T. A. Moustafa, M. El-Hanunady
Tomato spotted wilt virus was originally isolated from naturally infected tomato fruits. The virus infected a wide host range showing different symptoms varied from local necrosis, systemic necrosis, systemic mosaic yellowing, bronzing, or stunting. The virus…
4 Apr 2023 - Research Article
Purification and Serological Studies on Barley Stripe Mosaic Hordevirus
M.A.S. El-Kady, Om-Hashem M. El-Banna, E.A Salama, Salwa N. Zein
Three different procedures of purification were performed to purify a local isolate Of Barley stripe mosaic Hordevirus (BSMV). The 1st procedure was successful in producing relatively high yield of virus preparation 3.7 mg/100 g of barley leaves with adequate…
4 Apr 2023 - Research Article
Isolation and Identification of the Causal Virus Aucuba Disease in Egypt
A. S. Gamal El din, Sohair I. El-Afifi, A. S. Sadik, Nashwa M. A. Abd El Mohsen, H. M. Abdelmaksoud
The infected potato plants showed aucuba symptom characteristic to this virus (brilliant and extensive yellow mottle on lower and middle leaves reaches their tips, brown patches and sunken brown areas were frequently observed on the tubers surface) were used a…
4 Apr 2023 - Research Article
Identification of Sugarcane Mosaic Potyvirus Strains in Egypt
A.l. Abd El-Fattah, A.s. Saclik M.M. El-Kholi, I.A. Åbdcl-Hmnid, M.A. Madkour
Sugarcane mosaic pot.1Virus (SCMV) is the causal agent Of mosaic disease which is reported to be one of the most important viral diseases attacking sugarcane crop in Egypt. Therefore. the present work was designed to screen the SCMV strains in Egypt based on b…
11 Apr 2023 - Research Article
Identification of A Virus From Naturally Infected Garlic Plants
S.A. El-Kewey, R.A. Omar, S.A. Sidaros, Samaa Abd El-Khalik
The isolated virus was identified according to host range, symptomatology, differential hosts, virus stability, modes of transmission, and electron microscopy. The virus has a narrow host range. Dilution end point was found to be 10-2. Thermal inactivation poi…
4 Apr 2023 - Research Article
Human Papillomavirus in Spontaneous Abortion
Enas K. Abo El-Maged, Azza H. El-Salakwy, Safia A. El-Gamal, Gehn Allam
First trimester spontaneously aborted products of conception were obtained from 100 Egyptian women. Placental tissue specimens from 100 women of full term normal vaginal deliveries were used as a control group. Their age ranged from 17-42 years (mean = 29.31).…
4 Apr 2023 - Research Article
Large Scale Production of Potato Cirus Y Necrotic Strain (PVYN) Coat protein through Expression the CP Gene in E. coli
A.S. Gamal El din, Sohair I. El-Afifi, A. S. Sadik, Nashwa M. A. Abd El-Mohsen, H. M. Abdelmaksoud
A fragment with a size of 801 bp from the CP gene was amplified from Potato virus YN isolated from potato plants cultivated at Monofyia Governorate. RNA was extracted from virus-infected tissues of D. metel leaves by the use of degenerate primers through polym…
4 Apr 2023 - Research Article
Biological and Molecular Characterization of Different Isolates of Potato Virus Y (N Group)
A.Y. Ahmad, M. A. Amer, T. A. Mostafa, F. M. Abo El-Abbas, M. El-Hammady
Ten isolates of Potato virus Y (PVY) were collected from diseased potatoes grown at different locations in Egypt and some of their biological and molecular characters were studied using several techniques. Many different symptotypes were appeared on Chenopodiu…
4 Apr 2023 - Research Article
Immunocapture Polymerase Chain Reaction (IC-PCR) and Nucleic Acid Hybridzation Techniques for Detection of Spiroplasma citri
Om-Hashem M. El Banaa l, A.A. Abou Zeid, Fawzia I. Moursy, Azza, G. Farag
Spiroplasma citri Saglio et al is the type species of the genus spiroplasma. Spiralin gene is the gene responsible for spiralin which is the major protein of Spiroplasma citri (S. citri Saglio et al), the causal organism of citrus stubborn disease. RecA gene p…
4 Apr 2023 - Research Article
Reverse Transcripion-Polymerase chain Reaction Mediated Detection and Identification of Tobacco mosaic virus- Egyption Strain
Sahar. A. Shoman, B. A. Othman
A procedure involving reverse transcription followed by polymerase chain reaction (RT-PCR) with availability of the database sequences (GenBank) provided a sensitive method for the detection or Tobacco mosaic virus- Egyptian strain (TMV-E). Total RNA extract o…
4 Apr 2023 - Research Article
Occurrence of Apple Chlorotic Leaf Spot Trichovirus in Egypt
Salwa N. Zeinl, Hanaa S. Zawam
Apple chlorotic leaf spot virus (ACLSV) isolated from apple trees was successfully transmitted by dagger nematodes (Xiphinema spp) It was transmitted to bait plants grown in soil containing Xiphinema spp. nematodes, The virus was identified according to host r…
4 Apr 2023 - Research Article
Characterization of Tobacco Rattle Tobravirus from Kaki (Diospyros Kaki)
Saiwa N. Zein
Kaki (Diospyros kaki) trees, showing vein banding. upward rolling of leaves and stunting of growth were observed in the farm Of South Tahrir. Isolation trials revealed that these symptoms were induced by Tobacco rattle virus. The virus was transmitted by mecha…
11 Apr 2023