Issue · 2026
Pakistan J. Zool., Vol. 58, Iss. 5, pp. 2001-2257
28 articles · pp. 2001-2257 · All issues →
Research ArticleERIC-PCR Based Molecular Typing of Escherichia coli Recovered from Milk in Peshawar, Pakistan
Faryal Khattak, Kafeel Ahmad, Muhammad Jawad Ullah, Sawaira Huriya
Milk is frequently contaminated with Escherichia coli that could cause infections in human through the food chain. This study aimed to investigate genetic diversity among Escherichia coli isolates recovered from milk samples collected from shops in Peshawar, P…
15 Jul 2026DOI 10.17582/journal.pjz/20250101160056
Research ArticleProteomic Dysregulations of Infertile PCOS vs. Non PCOS Females via Label-Free Quantitative Mass Spectrometry
Sabeen Fatima, Soma Vankwani, Haq Nawaz Khan, Sajid Ali Rajput, Munazza Raza Mirza, Rehana Rehman
Infertility presents as a complex and multifaceted challenge, characterized by inability to conceive, even after a year of engaging in regular and unprotected intercourse. The current study aims to investigate the underlying molecular mechanism of infertility…
15 Jul 2026DOI 10.17582/journal.pjz/20250625050207
Research ArticleScanning Electron Microscope and Molecular Identification of Cypris pubera Female from AlUla, Kingdom of Saudi Arabia
Rayan M. Alansari, Amin A. Seleem, Ebtesam A. Yousef
The Cypris is thought to be the most ancient ostracod generic name. The first species of ostracod to be called by the Linnean binomial classification was Cypris pubera. Our studied species Cypris pubera was collected from AlUla, Saudi Arabia and has geographic…
15 Jul 2026DOI 10.17582/journal.pjz/20250911160602
Research ArticleEvaluation of Reference Gene Expression on Different Tissue in Adults of Bay Snook Petenia splendida
Carlos Alfonso Alvarez-González, Alejandra del Carmen Castillo-Collado, Gloria Asencio-Alcudia, Carlos Alfonso Frías-Quintana, Vicente Morales-Garcia, Carina Shianya Alvarez-Villagomez et al.
The tenguayaca mojarra Petenia splendida is a fish that belongs to the cichlid family and its distribution is located from the southeast of Mexico to Guatemala and Belize. Therefore, molecular biology studies, particularly gene expression, provide information…
25 Jul 2026DOI 10.17582/journal.pjz/20240416201858
Research ArticleAssessment of Heavy Metals in Water, Sediments and Different Organs of Schizothorax labiatus Collected from River Panjkora Dir Upper, Pakistan
Naseer Ahmad, Nasir Shuaib, Amin Ullah, Manzoor Alam Khan, Raqeeb Ullah, Sajid Ullah et al.
Heavy metals, though essential in trace amounts, pose significant ecological and health risks at elevated concentrations. This study assessed the concentrations of cadmium (Cd), lead (Pb), magnesium (Mg), nickel (Ni), and iron (Fe) in water, sediments, and tis…
25 Jul 2026DOI 10.17582/journal.pjz/20250905170911
Research ArticlePopulation Dynamics of Insect Fauna Associated with Brinjal Crop Grown in Peri-Urban Agroecosystems in District Faisalabad
M. Azeem Yousaf, Zain Ul Abdin, Syeda Eishah Tu Razia, Waseem Akram, Hammad Ahmad Khan, Hasooba Hira et al.
The present study investigated the population dynamics of the insect pests and their natural enemies of the brinjal crop in the peri-urban agroecosystem of district Faisalabad. For this purpose, brinjal crops were grown in nine different locations of district…
25 Jul 2026DOI 10.17582/journal.pjz/20230918062825
Research ArticleA Preliminary Survey of Japanese Weasel (Mustela itatsi) Diet and Spatial Utilization in an Anthropogenically Modified Wetland
Chiharu Koga, Kazutaka M. Takeshita
The Japanese weasel (Mustela itatsi), endemic and widely distributed in Japan, is declining in numbers, and it may become endangered if its habitat conditions change. This study investigated the diet and spatial utilization of Japanese weasels at Lake Yanaka,…
31 Jul 2026DOI 10.17582/journal.pjz/20251018153131
Research ArticleEvaluation of Some Immunological Biomarkers (TGF-β, IL-35, CD68) as Prediabetes Markers for the Early Diagnosis of Type 2 Diabetes
Hayder L.F. Al-Msaid, Murtadha M. Jawad, Alrehbawy Rehab
Type 2 diabetes is a complicated metabolic disorder that leads to resistance to insulin, high levels of blood sugar and inflammation that doesn’t produce pain. In this matched case-control study, metabolic, hormonal and immunological parameters…
31 Jul 2026DOI 10.17582/journal.pjz/20260108155329
Research ArticleMacrobenthic Polychaetes Community Structure and Diversity in Tidal Mudflats of Korangi Creek, Karachi
Faiza Iqbal, Safia Khanam, Javed Mustaquim
This study focuses on the structure of the polychaete community inhabiting tidal mudflats in Korangi Creek, Karachi. We collected polychaetes over a year, from January to December 2017, and found 2,797 individuals from 12 families and 14 taxa. Of these, the ei…
31 Jul 2026DOI 10.17582/journal.pjz/20240622140045
Research ArticleThe Influence of Habitat Factors on Relative Abundance of Roe Deer Capreolus pygargus During the Summer in a Baekdudaegan Protected Area in South Korea
Hyun-Su Hwang, Hyejin Kim, Hyoun-Gi Cha, Naeyoung Kim, Hyun-Chul Shin, Hyo Jeong Kwon et al.
Understanding the relationship between habitat variables and species-specific habitat preferences is crucial for effective species conservation and management. We investigated the effect of vegetation structure on the habitat preference of roe deer Capreolus p…
31 Jul 2026DOI 10.17582/journal.pjz/20250705005207
Research ArticleA Green Technology for Crab Fishery: Serial Collapsible Crab Trap
J. Amala Shajeeva, N. Neethiselvan, M. Kalaiarasan, N. Jayakumar, R. Shalini, S. Archana
Crab traps are generally considered an eco-friendly fishing gear with high selectivity and pose minimal damage to ecosystem upon proper usage. A study was conducted from April 2023 to March 2024 to evaluate the catching efficiency of serial collapsible crab tr…
3 Aug 2026DOI 10.17582/journal.pjz/20251128092803
Research ArticleZootechnical Performance and Body Composition of Clarias gariepinus (Burchell, 1822) Fingerlings Fed Local Feeds Enriched with Azomite
Arnauld S.M. Djissou, Théophile Godome, Daouda Konate, Osny Zocli, N. Darius Tossavi, Mamadou Alpha Balde et al.
The study was conducted to assess the impact of azomite, a natural mineral derived from volcanic ash, on the zootechnical performance of Clarias gariepinus fingerlings fed with formulated diets based on local by-products. The study lasted 28 days. A total of f…
3 Aug 2026DOI 10.17582/journal.pjz/20250708133855
Research ArticleThe Influence of Habitat Factors on Habitat Preference of Cranes During the Wintering Periods in the DMZ Border Area, South Korea
Hyun-Su Hwang, Myeong Cheol Kim, Hyungsoo Seo
The demilitarized zone (DMZ) border area has been subject to strict regulations on human activity for over 70 years due to military security concerns. These restrictions on human presence have allowed the area to develop diverse habitats with high biodiversity…
3 Aug 2026DOI 10.17582/journal.pjz/20251021040936
Research ArticleA Small Proportion of Agouti Hairs on Back, Contributing to Wild-Colored Coat Appearances in the Japanese House Mice
Masahiro A. Iwasa, Misaki Kurokawa
The proportions of the agouti and non-agouti hairs on the backs of skin specimens of wild-colored and black-colored coats from the wild-caught Japanese house mice and laboratory mice were analyzed. Hairs (> 50 hairs) were picked up from each position (3 mm2…
3 Aug 2026DOI 10.17582/journal.pjz/20251217235604- Short Communication
Optimizing Pest Management Strategies for Chickpea (Cicer arietinum) by Implementing Trap Barrier Systems Against Millardia meltada in Faisalabad, Pakistan
Hammad Ahmad Khan, Rashid Ali, Zeeshan Yousaf, Muhammad Ehsan Maqbool, Khadija Tul Kubra, Komal Riaz et al.
This study presents the results of management measures of Millardia meltada (Soft-furred field rat) regarding a one-acre chickpea crop (Cicer arietinum) in Faisalabad, Pakistan. For this, two (one acre in dimensions) adjacently growing chickpea crops were sele…
19 Aug 2026DOI 10.17582/journal.pjz/20230809052312 
ReviewPotential Use of Bypass Protein and Fat in Dairy Animal Diets: Challenges and Opportunities
Hossam M. Ebeid, Faiz-ul Hassan, Muhammad Asif Arain
During the lactation season, optimizing animal nutrition is crucial for dairy animals’ productivity. To address the limitations of ruminal breakdown, nutritionists focus on providing high-energy, protein-rich feed that bypasses ruminal digestion. The pri…
19 Aug 2026DOI 10.17582/journal.pjz/20240528125542
Research ArticleImpact of Electron Beam Irradiation Treatment on Jujube for Fruit Flies (Diptera: Tephritidae) Disinfestation and its Impact on Quality Parameters
Asad Rajput, Bashir Ahmed Kalhoro, Fahad Nazir Khoso, Kamran Nadeem, Rehana Naz Syed, Abdul Mubeen Lodhi
This study examined the effects of electron beam irradiation on jujube infested with Bactrocera zonata (Saunders) and B. dorsalis (Hendel). Infested fruits were treated with five different doses of electron beam irradiation (100, 200, 300, 400, and 500 Gy) and…
25 Jul 2026DOI 10.17582/journal.pjz/20250108161407
Research ArticleAntibiofilm Activity of Iron Nanoparticles Synthesised from Camellia sinensis against Bacterial and Fungal Pathogens
Tehreem Nasir, Aisha Waheed Qurashi, Syeda Shazia Bokahri, Uzma Rafi, Roheela Yasmeen
New and resistant pathogenic bacterial and fungal strains in agricultural field have raised the need to develop new compounds with broad range of antimicrobial activity. Therefore, present study was aimed for green synthesis of iron nanoparticles and testing t…
31 Jul 2026DOI 10.17582/journal.pjz/20200703120728
Research ArticleIL-1β Immune Response Gene Has Role Against Nematode Haemonchus contortus
Kavita Rawat, Aruna Pal, Subhasish Batabyal, Sammidha Banerjee, Naresh Kurechiya
Sheep are economically important livestock in India, but their productivity is severely affected by parasitic infections such as Haemonchus contortus. This study focused on characterizing the IL-1β gene in sheep and evaluating its role in immune response…
3 Aug 2026DOI 10.17582/journal.pjz/20250417151352- Short Communication
Effect of Sperm-Egg Ratio and Thawing Protocol on Hatching Success of Cryopreserved Rohu (Labeo rohita) Milt
Selvaraj Gokul, Dinesh Kumar, Santosh Kumar, Omdevsinh Wala, Irtiqa Hamdani
Cryopreservation of fish milt is an effective strategy to ensure year-round availability of quality gametes for aquaculture and conservation programs. However, fertilization efficiency using cryopreserved sperm is strongly influenced by sperm–egg ratio a…
19 Aug 2026DOI 10.17582/journal.pjz/20260218192115 
Research ArticleAssociation of TaqI VDR Polymorphism with the Response to Directly Acting Antiviral Treatment in Hepatitis C Patients
Noora Hassan Hezam Al-Aqmer, Soumble Zulfiqar, Muhammad Farooq Hanif, Abdul Rauf Shakoori, Sibgha Zulfiqar, Mateen Izhar
The objective of this study was to determine the association between TaqI Vitamin D receptor (VDR) polymorphism and the response to directly acting antiviral treatment in hepatitis C patients. This case control study included 132 participants, out of which 66…
25 Jul 2026DOI 10.17582/journal.pjz/20260110131646
Research ArticleForaging Ecology of Twelve Ardeid Species at Lungh Lake, Pakistan
Muhammad Nawaz Rajpar, Munir Ozturk, Salih Gucel, Iris Charalambidou
Ardeids are wading birds that play an important role in aquatic habitats, i.e., they are pest-control agents of amphibians, insects and small rodents and serve as bioindicators. They face overwhelming threats such as water pollution due to organic and inorgani…
31 Jul 2026DOI 10.17582/journal.pjz/20250511150332- Review
The Nickel Paradox: Its Ambivalent Role in Poultry Nutrition: A Review of Essentiality and Toxicity
Hurmat Amjad, Shahid Mahmood, Ghulam Murtaza, Saira Fiaz, Zarqa Mubeen, Narjis Batool et al.
Nickel is a heavy metal found throughout our surroundings and the crust of Earth. Nickel is frequently employed in several of the latest industrial techniques due to its distinct catalytic, magnetic, and optical capabilities, as well as its biological impacts.…
19 Aug 2026DOI 10.17582/journal.pjz/20250228174017 
Research ArticleAssessment of the Impact of Fenugreek and Flaxseed Oil Extracts on Male Reproductive Function in Albino Mice and Evaluation of Their Palatability in Mus musculus
Maha Mostafa, Amira Wael Mohammad, Reham I. Mohamed
Some medicinal plants have phytoestrogen compounds that act as natural estrogens. In this study, we investigated the antifertility effect of fenugreek seed and flaxseed oils on male albino mice as a prelude for their use in the control of house mice (Mus muscu…
3 Aug 2026DOI 10.17582/journal.pjz/20250425154323- Research Article
Effect of Buprenorphine and Butorphanol for Postoperative Analgesia Following Ovariohysterectomy in Cats
Sajjad Javaid, Hamad Bin Rashid, Ayesha Safdar, Mamoona Chaudhry, Abdul Aziz
The majority of anesthetic combinations that are used frequently do not offer adequate postoperative analgesia. Postoperative pain can cause stress and delay the healing of the surgical site. Opioids are the drug of choice for analgesia. This study was designe…
25 Jul 2026DOI 10.17582/journal.pjz/20241130143858 
Research ArticleEffect of Sex on Meat Quality of Dong’an Chicken (Gallus gallus)
Xu Liu, Hang Yang, Jiayi Cao, Xiangyong Qu
There is an increasing interest in free-range poultry with the increasing focus on animal welfare. The aim of this study is to examine the effect of sex on physicochemical traits, chemical composition, muscle fibers, mineral, amino acids and fatty acids conten…
14 Jul 2026DOI 10.17582/journal.pjz/20240910074724
Research ArticleUtilization of ICT Tools for Fishing by the Fishermen in Coastal Districts of Tamil Nadu State, India
P.N. Aishwarya, G. Arul Oli, N.V. Sujathkumar, T. Ravikumar, C. Lloyd Chrispin
The invasion of information and communication technology tools made the fisheries sector a greater extent of the economically viable behaviour towards fishing. The progression over the accession of information and communication technology (ICT) tools made fish…
14 Jul 2026DOI 10.17582/journal.pjz/20240813090458
Research ArticleDrug Resistance Detection and Class I integron Gene Cassette Analysis of Sika Deer Derived Escherichia coli
Hang Zhou, Yue Wang, Bingbing Guo, Rigaqiratu Wu, Wanying Sun, Dongyang Wang et al.
The current study shows that the resistance of Escherichia coli is closely related to the integron gene cassette. To evaluate the drug resistance and integron carriage of E. coli isolated from Sika deer in 4 deer farms in Heilongjiang Province, antibiotic resi…
14 Jul 2026DOI 10.17582/journal.pjz/20231112075059