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Whiteflies are Not Responsible for Transmission of Chickpea Chlorotic Dwarf Virus and Mastrebegomo Chimeric Virus

Whiteflies are Not Responsible for Transmission of Chickpea Chlorotic Dwarf Virus and Mastrebegomo Chimeric Virus

Sana Khalid1,2*, Muhammad Zia-ur-Rehman2,3, Usman Hameed2,4, Shabnum Shaheen1, Muhammad Naveed Shahid5, Khajista Jabeen1, Farah Khan1, Muhammad Saleem Haider1

1Department of Botany, Lahore College for Women University, Lahore, Pakistan
2Institute of Agricultural Sciences, University of the Punjab, Lahore, Pakistan
3Government Science College, Wahdat Road, Lahore, Pakistan
4Government Murray College, Sialkot, Pakistan
5Department of Botany, Division of Science and Technology, University of Education, Township, Lahore, Pakistan
 
* Corresponding author: sanakhalidpu@gmail.com

Fig. 1.

Agroinfiltration of different infectious clones in tobacco (Nicotiana benthamiana Domin.) and tomato (Solanum lycopersicum L.) plants (a) CpCDV (mastrevirus) (b) CLCuKoV-Bu with CLCuMB (begomovirus and betasatellite) (c) mastrebegomo chimeric virus.

Fig. 2.

PCR amplification of CLCuKoV-Bu -CP gene. Lane 1 shows no band visualized from infected tobacco plants. Lanes 2-3 shows ~ 771 bp CP gene of CLCuKoV-Bu in whiteflies and tomato plants, respectively. Lane M, 1kbp DNA ladder.

Fig. 3.

PCR amplification of full length genome of CLCuKoV-Bu. Lane M, 1kbp DNA ladder. Lanes 1-2, 2753 bp fragment of CLCuKoV-Bu full length fragment amplified by using BurFL-F and BurFL-R specific primers in whiteflies and infected tomato plants respectively. Lane 3, No CLCuKoV-Bu full length fragment is amplified from symptomatic tobacco plants.

Pakistan Journal of Zoology

August

Vol. 54, Iss. 4, Pages 1501-2001

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