Whiteflies are Not Responsible for Transmission of Chickpea Chlorotic Dwarf Virus and Mastrebegomo Chimeric Virus
Sana Khalid1,2*, Muhammad Zia-ur-Rehman2,3, Usman Hameed2,4, Shabnum Shaheen1, Muhammad Naveed Shahid5, Khajista Jabeen1, Farah Khan1, Muhammad Saleem Haider1
Agroinfiltration of different infectious clones in tobacco (Nicotiana benthamiana Domin.) and tomato (Solanum lycopersicum L.) plants (a) CpCDV (mastrevirus) (b) CLCuKoV-Bu with CLCuMB (begomovirus and betasatellite) (c) mastrebegomo chimeric virus.
PCR amplification of CLCuKoV-Bu -CP gene. Lane 1 shows no band visualized from infected tobacco plants. Lanes 2-3 shows ~ 771 bp CP gene of CLCuKoV-Bu in whiteflies and tomato plants, respectively. Lane M, 1kbp DNA ladder.
PCR amplification of full length genome of CLCuKoV-Bu. Lane M, 1kbp DNA ladder. Lanes 1-2, 2753 bp fragment of CLCuKoV-Bu full length fragment amplified by using BurFL-F and BurFL-R specific primers in whiteflies and infected tomato plants respectively. Lane 3, No CLCuKoV-Bu full length fragment is amplified from symptomatic tobacco plants.