Variations in the Bubaline Growth Hormone Gene in the Coding and Non-Coding Regions
Variations in the Bubaline Growth Hormone Gene in the Coding and Non-Coding Regions
Amtul Jamil Sami*, Sehrish Bilal and Syeda Anum Zahra
Sequence of GH gene from bubaline samples, which cover a part of the 5’-flanking region of the first exon. The amplified product can be seen to be 501bp size. TATA box is shown in orange and sequence of exon 1 is shown in blue. The highlighted sequence in turquoise shows the inverted repeat sequence. A single base change in repeated sequence is highlighted in Yellow.
Alignment of nucleotide sequence of product with Bubalus bubalis growth hormone (GH) gene (KC107770.1) using NCBI BLAST. The sequence aligned with position 1 to 246 of Bubalus GH reference sequence. Mutations are shown in turquoise color. Yellow shows the position of mutation in reference sequence. TATA box is shown in orange and sequence of exon 1 is shown in blue.
Alignment of nucleotide sequence of product with Bos mutus isolated yakQH1 chromosome 19 (CP027087.1) using NCBI BLAST. 90% of the sequence aligned with three regions of Yak. A, showing range 1 aligned with first 31 nucleotides. B, showing range 2 aligned with nucleotides from 85 to 421 nucleotides. C, showing range 3 aligned from position 422 to 478.Mutations are shown in turquoise color. Yellow shows the position of mutation in reference sequence.
Alignment of nucleotide sequence of product with Bos indicus bovine growth hormone (GH2) gene, GH2.1 allele (AY662651.1) using NCBI BLAST. 87% of the sequence aligned with three regions of Cattle. A, showing range 1 from position 31 to 58 aligned with first 28 nucleotides. B, showing range 2 from position 115 to 450 aligned from 85 to 420 nucleotides. C, showing range 3 from position 1 to 80 of gene aligned with position 422 to 478.Mutations are shown in turquoise color. Yellow shows the position of mutation in reference sequence.
