UVB Irradiation Induces Human Skin Fibroblasts Senescence Through Regulation of Β-Catenin and Smp30 Expression
UVB Irradiation Induces Human Skin Fibroblasts Senescence Through Regulation of Β-Catenin and Smp30 Expression
Feng-Mei Yang1, Rui Li2, Xiu-Xue Hu3, Yong Liang4, Bo Gao3* and Wei Chen3*
The changes of every detection indicator with different UVB doses for different durations. a, cell proliferation inhibition rate, CPIR; b, apoptosis rate; c, β-catenin mRNA expression; d, SMP30 mRNA expression.
The changes of HSF apoptosis with different UVB doses for different durations. A, different doses UVB treatment 1 day; B, different doses UVB treatment 2 day; C, different doses UVB treatment 3 day.
Analysis of the relationship between every detection indicator and different UVB doses for different durations by Bilinear regression. a, cell proliferation inhibition rate (CPIR); b, apoptosis rate; c, β-catenin; d, SMP30), P<0.05.
The expression ofβ-catenin and SMP30 mRNA /protein in every group. A, gene expression products by PCR: 1, control group; 2, GAPDH; 3~5, β-catenin; 6~8, SMP30; 9, Marker. B, protein expression products by Western Blot.
