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The Effect of PIS Region on Horned or Polled Phenotype Traits in China Guanzhong Dairy Goats

The Effect of PIS Region on Horned or Polled Phenotype Traits in China Guanzhong Dairy Goats

Zilu Zhang1, Ning Wang2, Ce Hou1, Laiba Shafique1, Saif ur Rehman1, Zhuyue Wu2, Zhiqiang Wang1, Mingsong Wei3* and Kuiqing Cui1*

1A State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi University, Nanning, Guangxi 530005, PR China
2Shaoxing Hospital of Traditional ChineseMedicine, No. 641 Renmin Middle Road, Shaoxing, 312000 Zhejiang, P.R. China
3The Animal Husbandry Research Institute of Guangxi Zhuang Autonomous Region, Nanning 53001, China
 
Zilu Zhang and Ning Wang are the joint first authors.
 
* Corresponding author: 326220543@qq.com; kqcui@126.com

Fig. 1.
PCR amplified fragments of PIS in Guanzhong dairy goats.
A, Horned and polled goats; B, The distribution of the primers in goat PIS region; C~G, PCR products of PIS whole; PIS1-1, PIS1-2, PIS2, PIS3. M, DL5000 Marker; P1-P3, PCR products of polled goats; H1-H3, PCR products of hornedgoats.
Fig. 2.

Blast results of the 12.814kb sequence (screenshot of partial results).

Fig. 3.

Some polymorphic sites of 12.814kb. P1-P3, Polled goats; H1-H3, horned goats.

Fig. 4.

The prediction result of 12.814kb sequence. A, prediction result of tRNA; B, Prediction result of microRNA; C, Prediction result of conserved domains; D, Prediction result of inverted repeats.

Fig. 5.

Annotation result of 12.814kb sequence. A, Annotation result of 12.814kb sequence; B, the location information of PIS in chromosome.

Pakistan Journal of Zoology

October

Pakistan J. Zool., Vol. 56, Iss. 5, pp. 2001-2500

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