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Taxonomic Divergence of Medically Important and Toxigenic Aspergillus minisclerotigenes from Aspergillus flavus

Taxonomic Divergence of Medically Important and Toxigenic Aspergillus minisclerotigenes from Aspergillus flavus

Amna Shoaib*, Zoia Arshad Awan and Naureen Akhtar

Institute of Agricultural Sciences, University of the Punjab, Lahore, Pakistan.

*Correspondence | Amna Shoaib, Institute of Agricultural Sciences, University of the Punjab, Lahore, Pakistan; Email: [email protected]

Figure 1:

Comparison of colonies grown on MEA front and reverse (a and c) and on CZ (b and d). Microscopic study of A. minisclerotigenes (e-g) and A. flavus (h-j) showing seriation (uniseriate and biseriate) and conidial attachment. Am: A. minisclerotigenes; Af: A. flavus.

Figure 2:

Comparative screening of aflatoxin production by A. minisclerotigenes and A. flavus grown on CCA. a: colony from front side; b: reverse colony; c: reverse colony under UV light. Am: A. minisclerotigenes; Af: A. flavus.

Figure 3:

Aflatoxins production on TLC. S: AFBs Standard, Am: A. minisclerotigenes and Af: A. flavus.

Figure 4:

Amplified ITS region of strains, M=1kb DNA marker; Af: A. flavusus and Am: A. minisclerotigenes.

Figure 5:

 ITS sequence alignment of A. minisclerotigenes.

Figure 6:

ITS sequence alignment of Aspergillus flavus.

Figure 7:

DNA banding profile of PCR-ISSR amplification product. M: DNA marker; Am: A. minisclerotigenes and Af: A. flavus.

Journal of Innovative Sciences

December

Vol.9, Iss.2, Pages 192-241

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