Taxonomic Divergence of Medically Important and Toxigenic Aspergillus minisclerotigenes from Aspergillus flavus
Amna Shoaib*, Zoia Arshad Awan and Naureen Akhtar
Institute of Agricultural Sciences, University of the Punjab, Lahore, Pakistan.
*Correspondence | Amna Shoaib, Institute of Agricultural Sciences, University of the Punjab, Lahore, Pakistan;
Email: [email protected]
Figure 1:
Comparison of colonies grown on MEA front and reverse (a and c) and on CZ (b and d). Microscopic study of A. minisclerotigenes (e-g) and A. flavus (h-j) showing seriation (uniseriate and biseriate) and conidial attachment. Am: A. minisclerotigenes; Af: A. flavus.
Figure 2:
Comparative screening of aflatoxin production by A. minisclerotigenes and A. flavus grown on CCA. a: colony from front side; b: reverse colony; c: reverse colony under UV light. Am: A. minisclerotigenes; Af: A. flavus.
Figure 3:
Aflatoxins production on TLC. S: AFBs Standard, Am: A. minisclerotigenes and Af: A. flavus.
Figure 4:
Amplified ITS region of strains, M=1kb DNA marker; Af: A. flavusus and Am: A. minisclerotigenes.
Figure 5:
ITS sequence alignment of A. minisclerotigenes.
Figure 6:
ITS sequence alignment of Aspergillus flavus.
Figure 7:
DNA banding profile of PCR-ISSR amplification product. M: DNA marker; Am: A. minisclerotigenes and Af: A. flavus.