Surveillance of Mycoplasma spp. Among Cases of Viral Respiratory Affections in Broiler Chickens in Egypt
Surveillance of Mycoplasma spp. Among Cases of Viral Respiratory Affections in Broiler Chickens in Egypt
Abdelrahman A. Abdelrahman1, Salama A. S. Shany1, Kareem E. Hassan1, Mansy A. A. Dardeer2, Ali A.1*, Magdy F. El-Kady1*
Diagnostic scheme of collected field samples. Abbreviations: IBV; infectious bronchitis, AIV-H9N2: avian influenza H9N2, MG; Mycoplasma gallisepticum, MS; Mycoplasma synovaie, PCR; polymerase chain reaction, PPLO; pleuropneumonia-like organisms, +ve; positive, -ve; negative.
Characteristic colony morphology of Mycoplasma spp. isolated from broiler chickens showing fried egg appearance under inverted microscope at 4× magnification (A) and 10× magnification (B).
PCR detection of 16S rRNA gene of Mycoplasma gallisepticum (185 bp) (A) and Mycoplasma synoviae (205-210 bp) (B) in samples collected from broiler chicken flocks. L; DNA ladder, PC; positive control; NC; negative control, 1-8; field samples.
Geographical distribution of samples in northern upper Egypt
