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Sequence and Morphology of Cysticercus pisiformis in Local Breed Rabbits

Sequence and Morphology of Cysticercus pisiformis in Local Breed Rabbits

Kadhim Kh. K. Al-Khayat1*, Athmar K. A. Al-Azawi2

1Department of Veterinary, Karbala Veterinary Hospital, Ministry of Agriculture, Iraq; 2Department of Parasitology, College of Veterinary Medicine, University of Baghdad, Iraq.

 
*Correspondence | Kadhim Kh. K. Al-Khayat, Department of Veterinary, Karbala Veterinary Hospital, Ministry of Agriculture, Iraq; Email: [email protected]

Figure 1:

Shape and measurements of Cysticercus pisiformis cysts, grossly.

Figure 2:

Structure of Cysticercus pisiformis Scolex staining by acetoacetic carmine stain, Suckers (red arrow) and Hooks (blue arrow), X40. 

Figure 3:

Agarose (1%) gel electrophoresis of PCR products for (NAD1) gene of C. pisiformis of rabbits and DNA Marker Ladder (M) (100- 1500bp), PCR products amplicons band lances (1, 7, 13, 18, 29, 31, 36, 41, 56 and 59) sized 500bp. (1h, 7 volt/cm2 TBE).

Figure 4:

Agarose(1%) gel electrophoresis of PCR products for (COX1) gene of C. pisiformis of rabbits and DNA Marker ladder (M) (100- 1500bp), PCR products amplicons band lances (1, 7, 13, 18, 29, 31, 36, 41, 56 and 59) sized 446bp. (1h, 7 volt/cm2 TBE).

Figure 5:

Phylogenetic tree study based on Taenia pisiformis NAD1 gene partial sequence.

Figure 6:

Phylogenetic tree study based on Taenia pisiformis COX1 gene partial sequence.

Advances in Animal and Veterinary Sciences

November

Vol. 12, Iss. 11, pp. 2062-2300

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