Sequence Amplicon Analysis of 18S rDNA Variable Regions (V3, V4 and V9) in Natural Strains of Sordaria fimicola and Response to Different Carbohydrates
Sequence Amplicon Analysis of 18S rDNA Variable Regions (V3, V4 and V9) in Natural Strains of Sordaria fimicola and Response to Different Carbohydrates
Rabia Arif*, Shahar Bano, Muhammad Ishfaq and Muhammad Saleem
V3 and V4 region analysis of 18S rRNA genes in natural strains of S. fimicola and their F1 progeny. M, DNA marker; S3, parental strain; 1, 2 and 3, F1 progeny of S3; N5, parental strain; 4, 5, 6 and 7, F1 progeny of N5; Neg., negative control without genomic DNA
CLUSTAL O (1.2.3) multiple sequence alignment of different strains of Sordaria fimicola with reference sequence of S. fimicola.
Circular Phylogram to find phylogenetic relationship among ascomycetes and experimental strains reconstructed from the conserved region spanning V3 and V4 rDNA genes (A) and V9 rDNA genes (B) yellow highlighted colour indicating strain under study.