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Screening, Characterization and Purification of Cellulase from Mango Mealybug Drosicha stebbingi Green (Homoptera: Margarodidae)

Screening, Characterization and Purification of Cellulase from Mango Mealybug Drosicha stebbingi Green (Homoptera: Margarodidae)

Mubasshir Sohail*, Qadeer Ahmed Soomro, Raza Muhammad, Muhammad Usman Asif and Imran Rauf

Plant Protection Division, Nuclear Institute of Agriculture, Tando Jam, Pakistan.

 
* Corresponding author: mubasshirsohailroy@gmail.com

Fig. 1.

Congo red plate assay of Drosicha stebbingi indication the presence of cellulase activity by formation of clear zone around the well at pH 5.8

Fig. 2.

Effect of pH (A), temperature (B) and substrate (C) on cellulase activity of D. stebbingi.

Fig. 3.

Effect of mono and divalent cations on cellulase activity of D. stebbingi.

Fig. 4.

Effect of EDTA (A) and β-mercaptoethanol (B) on cellulase activity of D. stebbingi.

Fig. 5.

Gel filtration for elution of cellulase on Sephadex G75 with flow rate 6 drops/minutes. 

Fig. 6.

Anion exchange chromatography for elution of cellulase active fractions on DEAE-Sepharose cl-6b with a flow rate of 10ml/h.

Fig. 7.

SDS-PAGE of Drosicha stebbingi cellulase. The arrows on right indicate the molecular weight of markers while left side arrow indicates the molecular weight of cellulase enzyme.

Pakistan Journal of Zoology

December

Vol. 54, Iss. 6, Pages 2501-3000

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