Characterization of Catalase form Carnivorous Fish Channa Striata Exposed to Binary Insecticides Mixture (Deltamethrin + Endosulfan)
Characterization of Catalase form Carnivorous Fish Channa Striata Exposed to Binary Insecticides Mixture (Deltamethrin + Endosulfan)
Sana Rana1, Sajid Abdullah1, Huma Naz2* and Khalid Abbas1
ABSTRACT
In this experiment toxic effect of deltamethrin(DM)+endoslfan(END) mixture on catalase (CAT) activity in gills of carnivorous fish Channa striata was observed. Fish were exposed to sub-lethal concentration (1/3rd of LC50) of DM+END mixture for 14-day. CAT was partially purified by using ammonium sulphate precipitation technique and kinetic characterization was also performed against different pH and temperature. CAT activity was measured spectrophotometrically at A240 nm. Results showed that highest CAT activity was noted from the crude extract of control fish gills (140.66±0.71UmL-1) as compared to DM+END exposed fish (99.23±0.71UmL-1). After desalted the CAT activity in gills of both control and insecticides exposed fish was calculated as 73.45±0.71 and 42.77±1.36UmL-1, respectively. The highest specific activity was observed in crude CAT extract from gills of control fish (112.53±0.28 Umg-1) as compared to exposed fish (82.69±1.12Umg-1). After desalted specific activity of gills CAT was lower in exposed fish (112.20±1.41Umg-1) in relation to control (170.80±1.41 Umg-1). Highest fold purification of CAT was noted in the gills of control C. striata (1.52±0.01) as compared to exposed fish (1.48±0.01). The percentage recovery of CAT for control and DM+END exposed fish was calculated as 52.21±1.41 and 43.10±1.41, respectively. Results further indicated that after each step of partial purification total protein contents and percentage recovery decreased from crude extract to desalted sample while fold purification was increased. The maximum activity of purified CAT was recorded at 6.0 pH and 30°C temperature for gills of C. striata. As the temperature was raised further the catalase activity decreased.
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