RPO30 Gene based PCR for Detection and Differentiation of Lumpy Skin Disease Virus and Sheep Poxvirus Field and Vaccinal Strains
RPO30 Gene based PCR for Detection and Differentiation of Lumpy Skin Disease Virus and Sheep Poxvirus Field and Vaccinal Strains
Sherin Reda Rouby
Generalized skin nodules in LSD diseased animal
Fore limb edema in LSD diseased calf
Enlargement of prescapular lymph node of a cow naturally infected with LSDV
Gel electrophoresis of RPO30 gene based PCR assay for detection and differentiation of LSDV and SPPV.
a: Lane 1: 100bp DNA ladder, Lane 2: 50bp DNA ladder, Lane 3: SPPV (VSVRI), Lane 4: SPPV (RM 65 strain), Lane 5, 6, 7: SPPV (field strains), Lane 8, 9: LSDV (Ticks), Lane10, 11, 12, 13: LSDV (scabs), Lane 14: LSDV (buffy coat), Lane 15: LSDV (Ismailia strain), Lane 1: 50bp DNA ladder, Lane 2: 100bp DNA ladder
b: Lane 1: 100bp DNA ladder, Lane 3: LSDV (Ismailia strain), Lane 4: LSDV (skin nodules), Lane 5: LSDV (lymph aspirate), Lane 6:12: LSDV (skin nodules), Lane 13: SPPV (VSVRI), Lane 14: control negative
