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LY6K Promotes Proliferation and Energy Metabolism of Lung Adenocarcinoma Cells by Regulating Aerobic Glycolysis

LY6K Promotes Proliferation and Energy Metabolism of Lung Adenocarcinoma Cells by Regulating Aerobic Glycolysis

Yan Wang1,2*, Lin Liu3, Na Li4, Yijun Zong1, Wei Liu1, Wenhua Xu1, Qi Wang1, Peijuan Zhang1 and Huiling Feng2,5*

1Department of Medical Nursing, College of Nursing, Hebei University of Chinese Medicine, China
2Key Laboratory for Health Care with Chinese Medicine of Hebei Province, Hebei University of Chinese Medicine, China
3Department of Biochemistry and Molecular Biology, College of Basic Medicine, Hebei University of Chinese Medicine, China
4Department of Oncology, Hebei General Hospital, China
5Department of Surgical Nursing, College of Nursing, Hebei University of Chinese Medicine, China
 
Yan Wang and Lin Liu contributed equally to this study.
 
* Corresponding author: wangyan_wy2022@126.com

Fig. 1.

Expression of LY6K gene in lung adenocarcinoma. A-B, Expression of LY6K mRNA in GEPLA2 and TCGA databases. C-E, Survival analysis of LY6K mRNA in Kaplan Meier plotter, GEPLA2 and TCGA databases.

Fig. 2.

Protein expression of LY6K in lung cancer cells. A, Protein expression of LY6K in lung cancer cells. B, protein quantification in A. **p <0.01; ***p <0.001.

Fig. 3.

Transfection efficiency. A, Expression of LY6K mRNA in A549 cells after transfection of si-LY6K, **p <0.01; B, Expression of LY6K protein in A549 cells after transfection of si-LY6K, ** p <0.01.

Fig. 4.

Effect of LY6K on proliferation of A549 cells. A, Effect of LY6K knockdown on proliferative activity of A549 cells, * p <0.05; B, Effect of LY6K knockdown on A549 cell cloning, ** p <0.01; C, Effects of LY6K knockdown on CyclinD1 and CDK4 in A549 cells, * p <0.05.

Fig. 5.

The effect of LY6K transfection on the expression of key glycolytic enzymes. A, The mRNA expression level of glycolytic related enzyme after LY6K knockdown in A549 cells,** p <0.01; B, Expression level of glycolytic-related enzyme protein after LY6K knockdown in A549 cells,** p <0.01,* p <0.05.

Fig. 6.

The effect of LY6K knockdown in A549 cells on tumor formation was observed in subcutaneous tumor carrying experiment in nude mice. A, Tumor growth curves of si-NC group and si-LY6K group, ** p <0.01; B-C, Tumor weight in the si-NC group and the si-LY6K group, ** p <0.01; D, Expression of LY6K, GLUT1, HK2, PFKL, ALDOA, PGK-1, PKM2 and LDHA in subcutaneous tumor tissue of nude mice in si-NC group and si-LY6K group, * p <0.05.

Fig. 7.

Effect of LY6K knockdown on glycolysis of A549 cells. A, Glucose content in cell culture medium after LY6K knockdown by A549 cells, * p <0.05; B, Lactate content in cell culture medium after LY6K knockdown by A549 cells, * p <0.05; C, ATP concentration in cell culture medium after LY6K knockdown by A549 cells, * p <0.05.

Pakistan Journal of Zoology

June

Pakistan J. Zool., Vol. 56, Iss. 3, pp. 1001-1500

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