Prokaryotic Expression, Purification, and Functional Characterization of the Large Yellow Croaker (Larimichthys crocea) Mannose Receptors Subunits (MRC1 and MRC2)
Xiangli Dong1,2, Shilin Mikhail Borisovich2, Jiji Li1,*, Jianyu He1, Zeqin Fu1, Yingying Ye1, Julia N. Lukina3, Olga V. Apalikova4 and Jianshe Zhang1
L.c-MRC1and L.c-MRC2 membrane protein analysis.
Epitope analysis of L.c-MRC1and L.c-MRC2.
DNA electrophoresis figures. A, PCR identification of L.c-MRC1and L.c-MRC2; B, colony PCR analysis of MRC1 and MRC2 pET32A vector clones; C, MRC1-pET32A and MRC2-pET32A plasmid electrophoresis; D, MRC1-pET32A and MRC2-pET32A vector restriction digests.
Protein electrophoresis figures. A, SDS-PAGE analysis of L.c-MRC1 and L.c-MRC2 proteins; B, SDS-PAGE analysis of precipitated L.c-MRC1 and L.c-MRC2 proteins (the black arrows indicate the target protein bands. The target protein is detected in the precipitate); C, Purified L.c-MRC1 and L.c-MRC2 proteins. The black arrows indicate the target protein bands.
3D protein structures of L.c-MRC1-C and L.c-MRC2-C. These protein structures were predicted using SWISSMODEL. The N-terminus is blue and the C-terminus is red (for interpretation of the references to color in this figure legend, the reader is referred to the web version of this article).