Prevalence of Mycotic Mastitis and Evaluation of Some Virulence Potential of Candida albicans Isolated from Mastitic Goats
Prevalence of Mycotic Mastitis and Evaluation of Some Virulence Potential of Candida albicans Isolated from Mastitic Goats
Saddam Hussein Mahmoud* and Shaimaa Nabhan Yassein
macroscopical characteristic of C. albicans cultured on Sabouraud dextrose agar for 72 hours at 37 °C. The resulting colony is a white cream pasty colony.
C. albicans pseudohyphae with chlamydospore/ after three days of growing on Corn Meal Agar containing 1% Tween 80 at 28°C. (40X).
C. albicans producing germ tubes after one hour of development in fresh human serum at 37 ºC. (40X).
Morphology of C. albicans on CHROMagar reveals leaf-green colored colonies.
Urease test: negative Urease test for C. albicans.
C. albicans’s phospholipase activity on egg yolk agar medium at 37°C for (24–48) hours. Illustrate the zone of inhibition around the colony.
C. albicans haemolysin activity on Sabouraud dextrose blood agar with 3% glucose at 37 ºC for 48 hours. Display a clear zone around the colony.
Microplate inoculated with C. albicans isolates with Negative control for detection of biofilm formation.
Gel electrophoresis for extracted DNA.
Agarose gel 1% electrophoresis picture with an electric current of 75 volts for 1 hour was performed, showing the PCR product analysis of the SC1 gene in C. albicans was a positive isolate. with a PCR product size of 658 bp, ladder (100-1500 bp).
PCR products of the PLB1 gene of C. albicans were electrophoresed on a 1% agarose gel at 75 volts for an hour, with a product size of 765 bp.
The C. albicans SAP1 gene gel electrophoresis on 1% agarose gel at 75 volts for an hour with a product size of 479 bp.
