Oral SS-14 DNA Vaccine is More Potent than Oral SS-28 DNA Vaccine in Promoting Rat Lactation
Oral SS-14 DNA Vaccine is More Potent than Oral SS-28 DNA Vaccine in Promoting Rat Lactation
Yan-Guo Han1,2, Jun-Hua Ye1, Qin-Tao Zhao1, Yong-Jie Huang2, Kai Li2, Yong-Fu Huang2 and Li Xu1,*
Map of construction of oral somatostatin (SS) DNA vaccines encoding different lengths somatostatin and delivered by attenuated Salmonella choleraesuis C500. A, Map of construction of oral SS-14 DNA vaccine fused tPA signal peptide and CpG adjuvant named C500 (ptCS/2SS-14-asd); B, Map of construction of oral SS-28 DNA vaccine fused tPA signal peptide and CpG adjuvant named C500 (ptCS/2SS-28-asd); C, Map of construction of oral empty vector vaccine fused CpG adjuvant named C500 (pCpG-asd).
Identification of recombinant SS DNA vaccines encoding different lengths somatostatin and empty vector vaccine by PCR. Lanes 1, 2 and 3, C500 (ptCS/2SS-28-asd), C500 (ptCS/2SS-14-asd) and C500 (pCpG-asd) recombinant DNA vaccines were amplified with T7 and BGH primers; Lane M, DL5000 DNA marker. Three bands shown in Lanes 1, 2 and 3 are tPA-CpG-HBsAg-S-2SS-28-FLAG (1086 bp), tPA-HBsAg-S-2SS-14-FLAG (1002 bp) and CpG (178 bp) fragment, respectively.
Anti-SS antibodies titers in T1, T2 and C groups immunized with C500 (ptCS/2SS-14-asd), C500 (ptCS/2SS-28-asd) and C500 (pCpG-asd), respectively at weeks 3 (first day after childbirth) and 7 (week 4 after childbirth) after primary immunization. Vaccinations were respectively orally given at weeks 0, 3 and 6 weeks after primary immunization. Data are presented as means ± SD. *p < 0.05.
Serum GH (A), PRL (B) and IGF-1 (C) concentrations (ng/mL, uIU/mL and ng/mL, respectively) in T1, T2 and C groups immunized with C500 (ptCS/2SS-14-asd), C500 (ptCS/2SS-28-asd) and C500 (pCpG-asd), respectively at weeks 0 (before mating), 5 (week 2 after childbirth) and 7 (week 4 after childbirth) after primary immunization. Data are presented as means ± SD. *p<0.05.
Body weight (g) of offspring rats in T1, T2 and C groups at weeks 0 (first day after childbirth), 2 (week 2 after childbirth) and 4 (week 4 after childbirth) after birth. Data are presented as means ± SD. *p < 0.05.
