Optimization of Size Exclusion Chromatography for the Purification and Quantification of Foot and Mouth Disease Virus Serotype “O”
Optimization of Size Exclusion Chromatography for the Purification and Quantification of Foot and Mouth Disease Virus Serotype “O”
Abdul Razak1, Imran Altaf1*, Aftab Ahmad Anjum1, Ali Raza Awan2 and Farhat Nazir Awan3
Fig. 1.
Normal BHK-21 cells (A); CPEs developed due to FMDV (B).
Fig. 2.
Resolution between biomarkers at bed height 32cm (A), 40cm (B) and 48cm (C).
Fig. 3.
Chromatogram of samples at 2% CV, flow rate at 0.5ml (A) and 0.7ml (B); 4% CV at 0.5ml (C) and 0.7ml (D); 6% at 0.5ml (E) and 0.7ml (F).
Fig. 4.
Chromatograms of 2-fold dilutions of FMDV and Analysis of chromatographic elute of FMDV through SDS-PAGE.
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