Mutant Screening of Metarhizium lepidiotae for Increased UV-Tolerance and Virulence
Mutant Screening of Metarhizium lepidiotae for Increased UV-Tolerance and Virulence
Wenyou Huang1, Dan Yü1, Song Huang1,2, Jian Xiao2, Ping Qi2, Anhua Song2* and Zhen Huang1*
A, Virulence of wild-type strain (Ml03) and fast growing UV-induced mutants UV-20a, UV-40b, and UV-60c; B, the percent mortality is given on 3 and 6 d post-infection. (B) Adjusted accumulative mortality of wild-type strain (Ml03) and MlUV-40b mutant (UV-40b) tested against 2nd instar P. xylostella. The percent mortality is given at concentration of 1.0x106 conidia/ml. Data are presented as Mean ± SE.
A, morphological features of wild-type strain (Ml03) and MlUV-40b mutant on PDA, CZA, SDA and SDAY (12 d). B, Vegetative growth of wild-type strain (Ml03) and MlUV-40b (UV-40b) mutant on CZA, PDA, SDA and SDAY. Data are presented as mean ± SE.
Secreted protein profiles of M. lepidiotae wild type strain (WT) and MlUV-40b mutant (UV). Cell free culture supernatants were isolated and analyzed by SDS- PAGE after 3, 4, 5, 6 and 7 d of culture as detailed in the methods section. Std, molecular weight standards.
HPLC analysis of secondary metabolites produced by wild type strain and MlUV-40b mutant in liquid culture.
Effect of UV exposure on germination of M. lepidiotae Ml03 wild-type strain and MlUV-40b mutant. Conidial germination was determined over the indicated time course for untreated (Ml03-0 and UV-40b-0) and cells exposed to UV radiation for 40 min (Ml03-40 and UV-40b-40) (as described in the “Materials and Methods” section). Data are presented as mean ± SE.
Morphological features of wild-type strain (Ml03) and MlUV-40b (UV-40b) mutant on PDA medium plus NaCl, Sorbitol (SOR), Congo Red (CR) and H2O2.
Adjusted cumulative mortality of ethyl acetate extracts from wild-type strain (Ml03) and MlUV-40b (UV-40b) mutant tested against 2nd instar P. xylostella. The percent mortality is given at concentration of 50 mg/L. Data are presented as Mean± SE.
