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Mutant Screening of Metarhizium lepidiotae for Increased UV-Tolerance and Virulence

Mutant Screening of Metarhizium lepidiotae for Increased UV-Tolerance and Virulence

Wenyou Huang1, Dan Yü1, Song Huang1,2, Jian Xiao2, Ping Qi2, Anhua Song2* and Zhen Huang1*

1Key Laboratory of Bio-Pesticide Creation and Application of Guangdong Province, College of Agriculture, South China Agricultural University, Guangzhou, China, 510642 
2Guangzhou Institute for Food Inspection, Guangzhou, China, 511400
 
Wenyou Huang, Dan Yü and Song Huang contributed equally to the work.

*      Corresponding author: hzscau@scau.edu.cn; 1297044803@qq.com

Fig. 1.

A, Virulence of wild-type strain (Ml03) and fast growing UV-induced mutants UV-20a, UV-40b, and UV-60c; B, the percent mortality is given on 3 and 6 d post-infection. (B) Adjusted accumulative mortality of wild-type strain (Ml03) and MlUV-40b mutant (UV-40b) tested against 2nd instar P. xylostella. The percent mortality is given at concentration of 1.0x106 conidia/ml. Data are presented as Mean ± SE.

Fig. 2.

A, morphological features of wild-type strain (Ml03) and MlUV-40b mutant on PDA, CZA, SDA and SDAY (12 d). B, Vegetative growth of wild-type strain (Ml03) and MlUV-40b (UV-40b) mutant on CZA, PDA, SDA and SDAY. Data are presented as mean ± SE.

Fig. 3.

Secreted protein profiles of M. lepidiotae wild type strain (WT) and MlUV-40b mutant (UV). Cell free culture supernatants were isolated and analyzed by SDS- PAGE after 3, 4, 5, 6 and 7 d of culture as detailed in the methods section. Std, molecular weight standards.

Fig. 4.

HPLC analysis of secondary metabolites produced by wild type strain and MlUV-40b mutant in liquid culture.

Fig. 5.

Effect of UV exposure on germination of M. lepidiotae Ml03 wild-type strain and MlUV-40b mutant. Conidial germination was determined over the indicated time course for untreated (Ml03-0 and UV-40b-0) and cells exposed to UV radiation for 40 min (Ml03-40 and UV-40b-40) (as described in the “Materials and Methods” section). Data are presented as mean ± SE.

Fig. 6.

Morphological features of wild-type strain (Ml03) and MlUV-40b (UV-40b) mutant on PDA medium plus NaCl, Sorbitol (SOR), Congo Red (CR) and H2O2.

Fig. 7.

Adjusted cumulative mortality of ethyl acetate extracts from wild-type strain (Ml03) and MlUV-40b (UV-40b) mutant tested against 2nd instar P. xylostella. The percent mortality is given at concentration of 50 mg/L. Data are presented as Mean± SE.

Pakistan Journal of Zoology

June

Vol. 53, Iss. 3, Pages 801-1200

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