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Mosquitocidal Activity of cyt Positive Bacillus thuringiensis Isolated from Soil Samples

Mosquitocidal Activity of cyt Positive Bacillus thuringiensis Isolated from Soil Samples

Rabia Faiz and Dilara A. Bukhari*

Department of Zoology, Government College University Lahore, Lahore 

*      Corresponding author: [email protected]

 

Fig. 1.

Gram staining of GCU Bt 4 isolate.

Fig. 2.

Endospore staining of GCU Bt 4 (a) 18 h, (b) 30 h and (c) 48 h of sporulation.

Fig. 3.

Gel electrophoresis of 469 bp fragment of cyt 2B gene of the six most toxic Bt isolates, where 1, 2, 3, 4, 5 and 6 represent 6 Bt isolates viz GCU Bt 1, 2, 3, 4, 5 and GCU Bt 6 respectively while M stands for DNA marker.

Fig. 4.

Dengrogram representing 16S rRNA gene relatedness of six most toxic B.t. isolates NBBt 1,2,3,4,5 and 6 with accession numbers KX611122, KX611120, KX611121, KY611803, KY612210 and KY611804 with already reported genes in the Genbank DNA database.

Pakistan Journal of Zoology

December

Pakistan J. Zool., Vol. 56, Iss. 6, pp. 2501-3000

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