Molecular Detection of Methicillin-resistant Staphylococcus aureus (MRSA) from a Clinical Case of Myiasis Wound
Pravin Mishra1, Md. Muket Mahmud2, Md. Ahosanul Haque Shahid2, Alamgir Hasan2, Vivek Kumar Yadav3 and Moinul Hasan1*
1Department of Surgery and Obstetrics, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh; 2Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh; 3Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh.
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Figure 1
Collection of swab sample from myiasis wound.
Figure 2
Fermentation of MSA by Staphylococcus aureus indicated by Smooth and convex formation of a yellowish colony.
Figure 3
Staining characteristics of bacteria: Grape like clusters were found which are the characteristics of Staphylococcus aureus.
Figure 4
Results of PCR of Staphylococcus aureus specific nuc gene (size=279 bp). Here, Lane 1: 100 bp ladder, Lane 2: negative control, Lane 3: positive control, Lane 4- amplified nuc gene of S. aureus.
Figure 5
Amplification of the mecA gene (size=533 bp). Here, Lane 1: 100 bp ladder, Lane 2: positive control, Lane 3: amplified mecA gene of Staphylococcus aureus.